Enhancement of IgE Antibody Production by Ovalbumin Aerosol in Mice

101

Experimental asthma in guinea pigs, as defined in 1937 by Kallós and Pagel, and the pertinent literature are reviewed. The relation between guinea pig asthma and human asthma bronchiale is established. The underlying mechanism in both processes is the misdirected (aberrant) formation of anaphylactic or reaginic (IgE) antibody against innocuous antigens, leading at renewed exposure to the antigen to release of mast cell mediators (such as histamine, SRS-A, eosinotactic peptides, PAF), bronchoconstriction, vasodilation and eosinophilic inflammation at the sites of antigen-antibody reactions in the tissues of the respiratory organs.

Section: IIsupporting

confidence: 77%

Experimental Asthma in Guinea Pigs Revisited

Kallós¹,

Kallós²

1984

101

“…The transient enhancement of anti-OA IgE anti body production after intraperitoneal immunization with OA in mice pretreated with aerosolized OA [2] was confirm ed also in the present experiments. An in significant increase of IgE antibody production fol lowing irradiation (table II, groups A and B) was not observed in our previous experiments [2], This dis crepancy might be due to the different nebulizer used.…”

Section: Discussionsupporting

confidence: 87%

“…Mice were adm inistered 1 % aerosolized OA for 6 min on day -7 and challenged intraperitoneally with 10 ug OA and 2 mg Al(OH)3 on day 0 [2], As shown in figure 3, subacute exposure to ozone (1, 2, or 4 weeks) showed a considerable suppression o f anti-OA IgE antibody production on day 7 in the groups exposed to ozone.…”

Section: Effect O F Ozone Exposure On Primary Anti-oa Ige Antibody Rementioning

confidence: 96%

“…Previously we reported the transient enhancem ent o f antiovalbum in IgE an tibody production after intraperitoneal immunization with ovalbum in (OA) in mice pretreated with 1 % aero solized OA for 6 min. Furtherm ore, using a haptencarrier system it has been shown that the enhance ment of IgE antibody production was by T helper cells [2], The purpose of this paper was to investigate the ef fects of ozone on the IgE antibody production through the respiratory tract by means of IgE anti body production after aerosolized antigen adm inis tration in ozone-exposed mice.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Suppression of IgE Antibody Production after Exposure to Ozone in Mice

Ozawa¹,

Fujimaki

Imai³

et al. 1985

Self Cite

The effect of ozone exposure on IgE antibody production with aerosolized ovalbumin (OA) administration was investigated in Balb/c mice. Mice were continuously exposed to 0.8 ppm ozone for 1, 2, or 4 weeks, respectively, and subsequently aerosolized OA was administered through the respiratory tract for 6 min with a nebulizer. The mice were then immunized intraperitoneally 1 week later with OA. IgE antibody production was suppressed in ozone-exposed mice. However, no significant difference in the primary IgE antibody production by intraperitoneal immunization alone with OA was observed between ozone-exposed mice and nonexposed mice. In order to elucidate the suppressive mechanism of IgE antibody production, hapten-carrier antigenic system was used. It is shown that the induction of helper T cell function was suppressed if the aerosolized carrier protein was administered before intraperitoneal immunization in the mice exposed to ozone. These results suggest that ozone exposure has the effect on the stage of administration of inhaled antigen and the quite insignificant effect on the IgE antibody production after intraperitoneal immunization with OA.

“…We previously demonstrated that a single exposure to 1 % aerosolized OA primed helper T cells [11]. A single aerosolized OA exposure is as effective in the secondary IgE antibody produc tion as the intraperitoneal injection of OA, though it failed to induce primary IgE antibody production.…”

Section: Discussionmentioning

confidence: 99%

Induction of IgE Antibody Production to Aerosolized Ovalbumin in Mice Treated Intratracheally with Aluminum Silicate

Fujimaki

Ozawa²,

Ashikawa³

et al. 1986

Self Cite

Induction of IgE antibody production was studied in mice with aerosolized antigen exposure and intratracheal instillation of adjuvants. BALB/c mice were immunized intratracheally with 10 μg ovalbumin (OA) adsorbed with or without aluminum silicate as an adjuvant. A primary IgE antibody production was observed in mice immunized with OA plus adjuvant, but not with OA alone. In mice immunized with OA plus adjuvant, a secondary IgE antibody production was observed after a booster of OA aerosol exposure on day 28. Mice were instilled intratracheally with aluminum silicate or aluminum hydroxide (alum) and subsequently exposed to aerosolized OA for 30 min. Primary and secondary anti-OA IgE antibody productions were obtained in the groups instilled with the adjuvants. After the intratracheal instillation of alum, aluminum remained in the mice for a long period. These results suggest that the intratracheal instillation of adjuvants is effective in the induction of IgE antibody production by aerosolized antigen exposure.