Enhancement of hyphae growth and medium optimization by Pleurotus eryngii-3 under submerged fermentation

Abstract

There were a variety of amino acids and polysaccharides in Pleurotus eryngii contained that could improve human immunity and prevent cancer. In this paper, the medium was screened and carton and nitrogen sources were screened and optimized as 40 g/L corn flour and soybean meal by P. eryngii-3. Then, the effect of pH, temperature, volume, and rotate speed were investigated, in which the suitable fermentation conditions were respectively pH = 6, 27 ℃, 90 mL of fermentation broth, and 150 r/min of rotate speed. Meanwhile, the transcription levels of lcc1, lcc5, lcc8, and lcc12 were up-regulated by 10.25-, 3.32-, 2.33- and 6.29-fold respectively after optimization. In addition, the box-behnken design was utilized to improve DCW (17.23 g/L). This research was optimized the medium and condition of fermentation by P. eryngii-3, which could shorten the ripening cycle of seeds and improve economic benefits for industries.