Enhancement of human induced pluripotent stem cells adhesion through multilayer laminin coating

Nie, Yongzhan; Wang, Weiwei; Xu, Xun; Zou, Jie; Bhuvanesh, Thanga; Schulz, Burkhard; Ma, Nan; Lendlein, Andreas

doi:10.3233/ch-189318

Cited by 2 publications

(3 citation statements)

References 38 publications

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“…Therefore, the above results demonstrated that the physical coating of laminin could functionalize the DPEM surface to improve the adhesion of DPSCs. This observation is in accordance with the reported result that the laminin-coated surface could provide the functional biochemical signals for enhancing cell attachment and proliferation (Nie et al, 2018).…”

Section: Discussionsupporting

confidence: 93%

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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Native dental pulp extracellular matrix (DPEM) has proven to be an effective biomaterial for dental pulp regeneration. However, as a significant extracellular matrix glycoprotein, partial laminins were lost during the decellularization process, which were essential for odontoblast differentiation. Thereby, this study investigated the feasibility of LN supplementation to improve the surface of DPEM for odontoblast layer regeneration. The influences of laminin on cell adhesion and odontogenic differentiation were evaluated in vitro. Then, we fabricated laminin-modified DPEM based on the physical coating strategy and observed the location and persistency of laminin coating by immunofluorescent staining. Finally, laminin-modified DPEM combined with treated dentin matrix (TDM) was transplanted in orthotopic jaw bone of beagles (n = 3) to assess the effect of LNs on dental pulp tissue regeneration. The in vitro results showed that laminins could improve the adhesion of dental pulp stem cells (DPSCs) and promoted DPSCs toward odontogenic differentiation. Continuous odontoblastic layer-like structure was observed in laminin-modified DPEM group, expressing the markers for odontoblastogenesis, dentine matrix protein-1 (DMP-1) and dentin sialophosphoprotein (DSPP). Overall, these studies demonstrate that the supplementation of laminins to DPEM contributes to the odontogenic differentiation of cells and to the formation of odontoblast layer in dental pulp regeneration.

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Section: Discussionsupporting

confidence: 93%

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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“…To maintain an undifferentiated state and capability for self-renewal within their physiologic niche, stem cells require contact with an immobilized environment consisting of a thin layer of ECM, which can be mimicked by coating substrates in vitro. Traditional protein coating approaches, such as physical adsorption, require a non-physiologic, high protein concentration (>50 µg/mL) [ 31 ] to assure efficient hiPSC expansion [ 17 ]. Here, we described a cost-effective low concentration (0.5 µg/mL) polyLN521-based coating system capable of providing the homogeneous protein distribution essential for mimicking the in vivo environment of the niche [ 32 , 33 ] without the need of any additional coating components.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, the LN isoforms LN511 and LN521 have been shown to be efficacious in generating and maintaining PSCs under these chemically-defined conditions [ 15 , 16 ]. The cells cultured in LN-based systems have shown evidence of efficient attachment, long-term self-renewal, pluripotent marker expression, normal karyotypes, and differentiation capabilities in vitro and in vivo [ 17 , 18 ]. Overall, these recent advances have led to the establishment of recombinant LN as a desirable coating for the cultivation of PSCs.…”

Section: Introductionmentioning

confidence: 99%

Polymerized Laminin-521: A Feasible Substrate for Expanding Induced Pluripotent Stem Cells at a Low Protein Concentration

Mesquita¹,

Leite

Morrissey³

et al. 2022

Cells

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Laminins (LNs) play a central role in the self-assembly and maintenance of basement membranes and are involved in critical interactions between cells and other extracellular matrix (ECM) proteins. Among the defined, xeno-free ECM culture matrices, LNs—namely LN521—have emerged as promising coating systems for the large-scale expansion of induced pluripotent stem cells (iPSCs). The biologic activity of LNs is enhanced by their acidification-induced self-polymerization into a cell-associated network called polylaminin (polyLN), which can recapitulate the native-like polymeric array in a cell-free system. Here, we show for the first time to our knowledge that polyLN521 displays a native-like hexagonal-like structure and that, at basal and low concentrations, it permits the large-scale expansion of human iPSCs. Human iPSCs expanded with polyLN521 maintained the pluripotent state and showed no impairment of karyotype stability or telomere length. These results suggest that low-concentration polyLN521 is a stable and cost-effective coating for large-scale iPSC expansion.

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Enhancement of human induced pluripotent stem cells adhesion through multilayer laminin coating

Cited by 2 publications

References 38 publications

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

Polymerized Laminin-521: A Feasible Substrate for Expanding Induced Pluripotent Stem Cells at a Low Protein Concentration

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