Enhancement of growth and regeneration efficiency from embryogenic callus cultures of Oncidium ‘Gower Ramsey’ by adjusting carbohydrate sources

Jheng, F.Y.; Do, Yi‐Yin; Liauh, Yuh-Waan; Chung, Jen-Ping; Huang, Pung‐Ling

doi:10.1016/j.plantsci.2006.01.016

Cited by 48 publications

(26 citation statements)

References 29 publications

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“…Wu et al (2004) investigated the effects of two groups of auxins and cytokinins on embryo formation from calluses obtained from the root sample, the authors noted that there was no PLB on the MS medium without 2,4-D, but they still induced callus from this medium. Our results are similar to the rate of callus formation of 76.19% on MS medium supplemented with 1 mg/l 2,4-D. Jheng et al (2006) added with 2,4-D in the lower concentration to culture medium affected cell proliferation, which was similar to that obtained with low concentrations of 2,4-D in this study. Experimentally on callus formation, high concentrations of 2,4-D should be not used as it was the cause of mutant formation (Arditti, 2008) and the callus appeared to mutate in long-term culture (Jheng et al, 2006).…”

Section: Methodssupporting

confidence: 90%

“…Our results are similar to the rate of callus formation of 76.19% on MS medium supplemented with 1 mg/l 2,4-D. Jheng et al (2006) added with 2,4-D in the lower concentration to culture medium affected cell proliferation, which was similar to that obtained with low concentrations of 2,4-D in this study. Experimentally on callus formation, high concentrations of 2,4-D should be not used as it was the cause of mutant formation (Arditti, 2008) and the callus appeared to mutate in long-term culture (Jheng et al, 2006). Effect of NAA on PLB Formation from Callus Study on PLBs formation by callus culture on MS medium supplemented with NAA was performed.…”

Section: Methodssupporting

confidence: 90%

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Rapid Micropropagation of Vu Nu Orchid (Oncidium Sp.) by Using Tissue Culture Technique

Minh

Khoa

Hoa

2017

CBUP

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Abstract:The high demand of Oncidium orchids leads us to find out efficient methods of propagating them. However, the propagation rate of traditional methods are low in nature and a hybrid seed is not genetically stable. Thus, plant cell biotechnology is examined as the most effective way to resolve the barrier of elite clone production. Shoot clusters were cultured on MS medium supplemented with 2,4-D (1 mg/l) for callus induction (76.19%) before induced callus was favoured for PLBs regeneration (98 PLBs/callus cluster) on MS medium supplemented with NAA (0.75 mg/l); the combination of BA (0.5 mg/l) and NAA (0.5 mg/l) was favoured for PLBs regeneration (28.18 PLBs/shoot cluster) from shoots cultivation. The PLBs (79.21 PLBs/PLB cluster) were then proliferated on MS medium supplemented with NAA (1 mg/l) and BA (1 mg/l) for shoot regeneration (12.42 shoots/PLBs cluster). Multiple-shoots were divided to 3-4 shoots/cluster for micropropagation on the MS medium supplemented with the combination of BA (0.25 mg/l) and NAA (0.25 mg/l) to reach 11.66 shoots/cluster. Shoots were finally separated to single-shoot for rooting on the MS medium supplemented with NAA (0.75 mg/l). A scheme for Oncidium micropropagation using PLBs culture techniques was set up.UDC Classification: 57.01; DOI: http://dx

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Section: Methodssupporting

confidence: 90%

Section: Methodssupporting

confidence: 90%

Rapid Micropropagation of Vu Nu Orchid (Oncidium Sp.) by Using Tissue Culture Technique

Minh

Khoa

Hoa

2017

CBUP

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“…The ascendancy of TDZ in inducing somatic embryos (infant PLBs) from leaf explants was recounted in numerous orchid species including Oncidium (Mayer, Stancato, & Appezzato-Da-Glória, 2010), Doritaenopsis (Park, Yeung, Chakrabarty, & Paek, 2002), Dendrobium and Phalaenopsis (Kuo, Chen, & Chang, 2005). TDZ is also useful for rapid plant regeneration through organogenesis (Kishor & Devi, 2009;Jitsopakul, Thammasiri, & Ishikawa, 2013), somatic em-bryogenesis (Jheng, Do, Liauh, Chung, & Huang, 2006;Gow, Chen, & Chang, 2009), or PLB formation of orchids (Mayer, Stancato, & Appezzato-Da-Glória, 2010;Khoddamzadeh et al, 2011;Roy, Sajeev, Pattanayak, & Deka, 2012). NAA is a synthetic auxin plant growth regulator that is routinely used in in vitro propagation of orchid to promote callus induction such as in Dendrobium (Mei, Danial, Mahmood, & Subramaniam, 2012;Salam, Salam, & Mohanty, 2013), Vanilla (Tan, Chin, & Alderson, 2011), Oncidium (Mayer, Stancato, & AppezzatoDa-Glória, 2010), Phalaenopsis (Khoddamzadeh et al, 2011), and Renanthera (Wu et al, 2012).…”

Section: Effect Of Pgrs On Embryogenic Callus Induction From Leaf Tipmentioning

confidence: 99%

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

Jainol

Gansau

2017

Agrivita.J.Agr.Sci

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Dimorphorchis lowii is a rare epiphytic orchid endemic to Borneo and very popular due to its ornamental value. For the purpose of mass propagation, procedures for in vitro propagation through callus were developed. The objectives of this study were to determine the effects of plant growth regulators (PGRs) on callus induction from leaf tip explant and the effects of complex additives on PLBs formation and shoot development. The best combination of PGR was 3.0 mg L -1 Thidiazuron (TDZ) with 0.046 mg L -1 NAA supplemented in half-strength medium (½ MS) (Murashige & Skoog, 1962). The percentage of survival and callus formation obtained were 96.0 % ± 19.8 and 52.0 % ± 16.5 respectively. Maximum shoot proliferation from PLBs was observed in Knudson C (KC) medium enriched with 15 % (v/v) coconut water. In this treatment, 10.2 ± 6.2 shoots were produced from one callus explant. Shoots were rooted on KC medium containing 15 % (v/v) coconut water and transferred in a medium mixture containing sphagnum moss, charcoal, brick and coco peat with the ratio of 1:1:1:1. After 2 months being acclimatized in the glasshouse, 78 % of plantlets survived. Histology observations showed that embryogenic callus derived from leaf tip explants might originate from epidermis and mesophyll cells and was capable of developing into complete plantlets.

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“…An optimal level of auxin was found to be required for induction of regeneration and cell proliferation in TDZ-exposed geranium hypocotyls . Combination levels of exogenous 2, 4-dichlorophenoxyacetic acid (2,4-D) and thidiazuron (TDZ) changed granular and yellow callus of Oncidium into more friable or compact embryogenic callus and the most significant proliferation of this callus was observed after the third subculture (Jheng et al, 2006). Histological studies observed that cell proliferation is enhanced by TDZ with 2, 4-D rather than TDZ with IBA.…”

Section: Involvement Of Plant Growth Regulatorsmentioning

confidence: 99%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

195

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Thidiazuron (TDZ) has gained a considerable attention during past decades due to its efficient role in plant cell and tissue culture. Wide array of physiological responses were observed in response to TDZapplication in different plant species. TDZ has shown both auxin and cytokinin like effects, although, chemically, it is totally different from commonly used auxins and cytokinins. A number of biological (physiological and biochemical) events in cells are induced or enhanced by TDZ, but the mode of action of TDZ is yet unknown. However, varieties of underlying mechanisms were revealed by reports showing how morphogenic events were induced by application of TDZ. Other reports showed that TDZ may modify endogenous plant growth regulators, either directly or indirectly and produce reactions in cell/tissue, necessary for its division/regeneration. Other possibilities include modification in cell membrane, energy levels, nutrient absorption, transport and assimilation, etc. In this review, recent advancements in TDZ application in plant sciences are discussed.

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Enhancement of growth and regeneration efficiency from embryogenic callus cultures of Oncidium ‘Gower Ramsey’ by adjusting carbohydrate sources

Cited by 48 publications

References 29 publications

Rapid Micropropagation of Vu Nu Orchid (Oncidium Sp.) by Using Tissue Culture Technique

Rapid Micropropagation of Vu Nu Orchid (Oncidium Sp.) by Using Tissue Culture Technique

Embryogenic Callus Induction from Leaf Tip Explants and Protocorm-Like Body Formation and Shoot Proliferation of Dimorphorchis lowii: Borneon Endemic Orchid

Thidiazuron: A multi-dimensional plant growth regulator

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