Enhancement of growth and production of polyhydroxyalkanoates by<i>Bacillus subtilis</i>from agro-industrial waste as carbon substrates

Anjali, Mascarenhas; Sukumar, C.; Kanakalakshmi, A.; Shanthi, K.

doi:10.1080/15685543.2013.834200

Cited by 24 publications

(7 citation statements)

References 9 publications

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“…PHA is regarded as extremely competitive alternative to replace the petrochemical plastics, yet its high cost is always a major impediment. There have been numerous researches working on the exploitation of low-cost substrates and novel production hosts ( Anjali et al, 2014 ; Vibhavee and Chanprateep, 2018 ). As an industrial raw material, starch has been applied for the production of high value-added products such as gas or liquid fuels, proteins, and sugars ( Erdei et al, 2010 ; Lin et al, 2021 ; Li et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch

Tian²,

Lian³

et al. 2023

Front. Bioeng. Biotechnol.

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Vibrio alginolyticus LHF01 was engineered to efficiently produce poly-3-hydroxybutyrate (PHB) from starch in this study. Firstly, the ability of Vibrio alginolyticus LHF01 to directly accumulate PHB using soluble starch as the carbon source was explored, and the highest PHB titer of 2.06 g/L was obtained in 18 h shake flask cultivation. Then, with the analysis of genomic information of V. alginolyticus LHF01, the PHB synthesis operon and amylase genes were identified. Subsequently, the effects of overexpressing PHB synthesis operon and amylase on PHB production were studied. Especially, with the co-expression of PHB synthesis operon and amylase, the starch consumption rate was improved and the PHB titer was more than doubled. The addition of 20 g/L insoluble corn starch could be exhausted in 6-7 h cultivation, and the PHB titer was 4.32 g/L. To the best of our knowledge, V. alginolyticus was firstly engineered to produce PHB with the direct utilization of starch, and this stain can be considered as a novel host to produce PHB using starch as the raw material.

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Section: Resultsmentioning

confidence: 99%

Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch

Tian²,

Lian³

et al. 2023

Front. Bioeng. Biotechnol.

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“…For assessing PHA loss during cell fixation and storage, pure cultures of Bacillus subtilis and Pseudomonas aeruginosa were used as representatives of Gram-positive and Gram-negative, PHA producing, model organisms. B. subtilis and P. aeruginosa were grown aerobically in media known to give a high PHA yield [47, 48].…”

Section: Methodsmentioning

confidence: 99%

Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH

et al. 2019

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Enhanced biological phosphorus removal (EBPR) is a globally important biotechnological process and relies on the massive accumulation of phosphate within special microorganisms. Candidatus Accumulibacter conform to the classical physiology model for polyphosphate accumulating organisms and are widely believed to be the most important player for the process in full-scale EBPR systems. However, it was impossible till now to quantify the contribution of specific microbial clades to EBPR. In this study, we have developed a new tool to directly link the identity of microbial cells to the absolute quantification of intracellular poly-P and other polymers under in situ conditions, and applied it to eight full-scale EBPR plants. Besides Ca. Accumulibacter, members of the genus Tetrasphaera were found to be important microbes for P accumulation, and in six plants they were the most important. As these Tetrasphaera cells did not exhibit the classical phenotype of poly-P accumulating microbes, our entire understanding of the microbiology of the EBPR process has to be revised. Furthermore, our new single-cell approach can now also be applied to quantify storage polymer dynamics in individual populations in situ in other ecosystems and might become a valuable tool for many environmental microbiologists.

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“…For assessing PHA loss during cell fixation and storage, pure cultures of Bacillus subtilis and Pseudomonas aeruginosa were used as representatives of Gram-positive and Gram-negative, PHA producing, model organisms. B. subtilis and P.aeruginosa were grown aerobically in media known to give a high PHA yield 48, 49 .…”

Section: Methodsmentioning

confidence: 99%

Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman-FISH

Fernando

McIlroy

Nierychlo

et al. 2018

Preprint

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18Enhanced biological phosphorus removal (EBPR) is a globally important biotechnological 19 process and relies on the massive accumulation of phosphate within special microorganisms. 20 Candidatus Accumulibacter conform to the classical physiology model for polyphosphate 21 accumulating organisms and are widely believed to be the most important player for the 22 33 34 Keywords: Polyphosphate accumulating organisms, Raman microspectroscopy, Fluorescence 35 in-situ hybridization, enhanced biological phosphorus removal 36 37 109 in these systems. 110 111 Materials and Methods 112

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Enhancement of growth and production of polyhydroxyalkanoates byBacillus subtilisfrom agro-industrial waste as carbon substrates

Cited by 24 publications

References 9 publications

Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch

Engineering Vibrio alginolyticus as a novel chassis for PHB production from starch

Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman–FISH

Resolving the individual contribution of key microbial populations to enhanced biological phosphorus removal with Raman-FISH

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