Enhancement of glycerol production by UV-mutagenesis of the marine yeast Wickerhamomyces anomalus HH16: kinetics and optimization of the fermentation process

Hawary, Heba; Rasmey, Abdel‐Hamied M.; Aboseidah, Akram A.; El-Morsi, El-Shahat; Hafez, M. B.

doi:10.1007/s13205-019-1981-4

Cited by 18 publications

(14 citation statements)

References 62 publications

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“…The acyl‐ACP molecule continues through the cycle until the appropriate chain length is reached. The enzymes involved in these reactions might be activated with cofactors present in peptone while not being available in other nitrogen sources (Hawary et al ).…”

Section: Discussionmentioning

confidence: 99%

Direct transesterification of fatty acids produced by Fusarium solani for biodiesel production: effect of carbon and nitrogen on lipid accumulation in the fungal biomass

2019

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Aims The present work aims to explore a new oleaginous Fusarium isolate potential to accumulate lipids in its biomass from inexpensive substrates. In addition, impacts of carbon and nitrogen sources and their ratios on lipid production by the interested fungal isolate were also studied. Methods and Results Lipid was assayed by sulfo‐phospho‐vanillin colorimetric method. Among 11 Fusarium isolates obtained on potato dextrose agar from rhizosphereic soils, Fusarium RAS18 was selected as the highest producer that accumulates above 20% lipid. It was identified based on phenotypic characterization and the internal transcribed spacer sequence as Fusarium solani, that was recorded in the GenBank database under the accession number . The optimized lipid yield (34·5%) is obtained using glycerol (35 g l−1) and peptone (1·5 g l−1) as carbon and nitrogen sources respectively. The produced fatty acid methyl esters (biodiesel) is composed of linoleic acid (56·81%), palmitic acid (17·81%), oleic acid (11·81%) and stearic acid (11·12). The unsaturated fatty acids accounted for 69% and this is nearly similar to the plant oils commonly used in biodiesel production. Conclusions These findings suggest the applicability of F. solani RAS18 as a promising strain to accumulate lipids from glycerol as a feedstock for biodiesel production. Significance and Impact of the Study Fusarium solani RAS18 is a new oleaginous fungal isolate that is able to produce lipid (34·5%, g g−1) from glycerol. Glycerol is a cheap substrate and is formed as a byproduct from transesterification process and others industries. Thus, recyclation of glycerol for lipid production by micro‐organisms is an important point of economic view. Direct transesterification of the produced fatty acids indicated its similarity to the plant oil composition used in biodiesel production. So, F. solani RAS18 might be a potential lipid source as a feedstock for biodiesel production.

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Section: Discussionmentioning

confidence: 99%

Direct transesterification of fatty acids produced by Fusarium solani for biodiesel production: effect of carbon and nitrogen on lipid accumulation in the fungal biomass

2019

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“…Higher glycerol yields have been obtained from species that show a relatively strong tolerance for high concentration of sugars and salts (Loray et al, 1995;Patil, 2013). Selection of yeast strains and optimization of fermentation conditions such especially the carbon source are critical parameters for glycerol overproduction (Ehsani et al, 2009;Orlic et al,2010;Mirończuk et al, 2016;Singh et al, 2016;Hawary et al, 2019). In this study, screening of some marine yeast isolates for glycerol production was performed.…”

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confidence: 99%

Kinetic models application on glycerol production from glucose by the marine yeast Candida orthopsilosis

Rasmey¹,

Aboseidah²,

Khalaf³

et al. 2020

Egypt. J. of Aquatic Biolo. and Fish.

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“…The yeast strain Wickerhamomyces anomalus HH16-MU5 used in the present study, is UV-mutant of the wild yeast W. anomalus HH16 which have been isolated from marine sediment, UV-treated and tested for glycerol production previously [5].…”

Section: Microorganism Sourcementioning

confidence: 99%

“…According to [28], cellulases isolated from marine fungi, especially yeast, have shown their potential for bioconversion processes which can have role in bioenergy-based industries. Several marine yeast genera such as Aureobasidium, Candida, Rhodotorula and Wickerhamomyces could be a source of cellulases and other valuable hydrolytic enzymes [5,[29][30].…”

Section: Effect Of the Simultaneous Saccharification And Fermentationmentioning

confidence: 99%

“…The most predominant polyol as an osmolyte in marine yeasts is the glycerol [4]. Glycerol (1,2,3-propanetriol) is used in manufacturing of food additives, cosmetics, paints, paper and leather as well as in the production of pharmaceutical formulating agents [5]. Moreover, it is a significant precursor for production of appreciated chemicals such as biodiesel, butanol, hydrogen, acrolein, citric acid, dichloro-2-propanol (DCP), 1,3-propanediol (1,3-PD), and 1,3-dihydroxyacetone (DHA) [1].…”

Section: Introductionmentioning

confidence: 99%

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Glycerol production by the UV-mutant marine yeast Wickerhamomyces anomalus HH16-MU15 via simultaneous saccharification and fermentation of fruit peels

Rasmey

Hawary

Abu-seidah

et al. 2020

Frontiers in Scientific Research and Technology

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Agro-industrial wastes in Egypt are indefinite and sustainable substrate for production of numerous industrially significant products. Using of these wastes as raw materials can reduce both the production cost and the environmental pollution. The present research aims to produce glycerol from fruit peels by the UV-mutant marine yeast Wickerhamomyces anomalus HH16-MU15. Fruits (banana, orange, mango and pomegranate) peels were pretreated by mechanical and hydrothermal methods before their fermentation into glycerol. The maximum total reducing sugars (5.792 ± 0.16gl-1) was obtained from mango peels followed by orange peels (5.33 ± 0.21gl-1) after their hydrothermally pretreatment. After fermentation, the maximum cellulase activity (8.911 Uml-1), total reducing sugars (48.59 gl-1) and glycerol yield (35.25 gl-1) by W. anomalus HH16-MU15 were achieved in the hydrolysate of hydrothermally pretreated orange peels at 96 hrs. The current investigation suggests the using of orange peels for production of glycerol and other valuable products by W. anomalus HH16-MU15.

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Enhancement of glycerol production by UV-mutagenesis of the marine yeast Wickerhamomyces anomalus HH16: kinetics and optimization of the fermentation process

Cited by 18 publications

References 62 publications

Direct transesterification of fatty acids produced by Fusarium solani for biodiesel production: effect of carbon and nitrogen on lipid accumulation in the fungal biomass

Direct transesterification of fatty acids produced by Fusarium solani for biodiesel production: effect of carbon and nitrogen on lipid accumulation in the fungal biomass

Kinetic models application on glycerol production from glucose by the marine yeast Candida orthopsilosis

Glycerol production by the UV-mutant marine yeast Wickerhamomyces anomalus HH16-MU15 via simultaneous saccharification and fermentation of fruit peels

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