Enhancement of glucose oxidase fermentation by addition of hydrocarbons

Li, Tai-Hsing; Chen, Tehy-Liang

doi:10.1016/0922-338x(94)90361-1

Cited by 21 publications

(3 citation statements)

References 16 publications

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“…Experimental evidence showed an increase in agitator speed corresponding to a higher concentration of dissolved oxygen, resulting in a higher growth rate and an increased production of GOX [ 58 ]. In addition, it is reported that the addition of hydrocarbons (n-dodecane, n-hexadecane) had a positive effect on the formation of intracellular and extracellular GOX [ 59 ]. These examples provide enough information for culturing a GOX-producing strain.…”

Section: Strategies For Improving Glucose Oxidase Yieldsmentioning

confidence: 99%

Insights into the Structures, Inhibitors, and Improvement Strategies of Glucose Oxidase

Wang

Chen

Wang

et al. 2022

IJMS

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Glucose oxidase, which uses molecular oxygen as an electron acceptor to specifically catalyze the conversion of β-d-glucose to gluconic acid and hydrogen peroxide (H2O2), has been considered an important enzyme in increasing environmental sustainability and food security. However, achieving the high yield, low price and high activity required for commercial viability remains challenging. In this review, we first present a brief introduction, looking at the sources, characteristics, catalytic process, and applications of glucose oxidase. Then, the predictive structures of glucose oxidase from two different sources are comparatively discussed. We summarize the inhibitors of glucose oxidase. Finally, we highlight how the production of glucose oxidase can be improved by optimizing the culture conditions and microbial metabolic engineering.

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Section: Strategies For Improving Glucose Oxidase Yieldsmentioning

confidence: 99%

Insights into the Structures, Inhibitors, and Improvement Strategies of Glucose Oxidase

Wang

Chen

Wang

et al. 2022

IJMS

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“…A number of liquid hydrocarbons have been successfully used as oxygen-vectors in aerobic fermentation (Li and Chen, 1994). In the present study three different oxygen-vectors were tested on the basis of their properties and influence on fermentation.…”

Section: Screening and Selection Of Oxygen-vectorsmentioning

confidence: 99%

Improved production of l-asparaginase by Bacillus brevis cultivated in the presence of oxygen-vectors

Narta

Roy

Kanwar

et al. 2011

Bioresource Technology

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“…The occurrence of foaming is facilitated by the existence of surfactants on the airwater interface, in which the surfactants are synthesized by the cells during the fermentation. n-Hexadecane can function as an antifoam agent because it can adsorb the surfactants (12)(13)(14), and the efficiency of antifoaming is obviously related to the oil-water interfacial area. Accordingly, the ability of the medium to repress foaming is relatively poor if most n-hexadecane is adsorbed by the cloth.…”

Section: Resultsmentioning

confidence: 99%

Lipase Production by Acinetobacter radioresistens in the Presence of a Nonwoven Fabric

Shen

Chen

et al. 1999

Biotechnology Progress

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Lipase production by Acinetobacter radioresistens was performed in a 2-L tank fermentor equipped with a nonwoven fabric, which was made of nylon 6 fiber and coated with a hydrophobic acrylic resin. The fermentation medium contained 2% (v/v) n-hexadecane as the carbon source. The use of the nonwoven fabric was intended for the dispersion of hydrocarbons; thus, the contact surface for the cells to assimilate n-hexadecane can be increased without using emulsifiers. The formation of lipase was found to be growth-associated when the cells grew on n-hexadecane. The use of the nonwoven fabric increased the lipase yield by 130%. Further supplementation of 0.1% (v/v) olive oil to the medium could markedly shorten the fermentation time, and thus increase the volumetric productivity. The use of the nonwoven fabric offered two additional advantages in the lipase fermentation: ease of foam control and favorable partition of lipase in the aqueous phase.

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Enhancement of glucose oxidase fermentation by addition of hydrocarbons

Cited by 21 publications

References 16 publications

Insights into the Structures, Inhibitors, and Improvement Strategies of Glucose Oxidase

Insights into the Structures, Inhibitors, and Improvement Strategies of Glucose Oxidase

Improved production of l-asparaginase by Bacillus brevis cultivated in the presence of oxygen-vectors

Lipase Production by Acinetobacter radioresistens in the Presence of a Nonwoven Fabric

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