Enhancement of ganoderic acid production by constitutively expressing Vitreoscilla hemoglobin gene in Ganoderma lucidum

Li, Huanjun; He, Yilong; Zhang, De-Huai; Yue, Tong-Hui; Lei, Jiang; Li, Na; Xu, Junwei

doi:10.1016/j.jbiotec.2016.04.017

Cited by 34 publications

(28 citation statements)

References 38 publications

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“…No significant difference was observed between WT and VHb in the liquid static culture mode. This may be due to the sufficient supply of oxygen and a relatively low cell density in the WT and VHb‐expressing strain under the normal culture condition (Li et al ., ).…”

Section: Resultsmentioning

confidence: 99%

“…Oxidative modification of the lanostane skeleton plays an important role in the biosynthesis of GA. The increased ratio of type I to type II GAs may be due to the stimulated activities of oxygenase involved in the GA biosynthesis pathway (Stark et al ., ; Li et al ., ). The transcription levels of cyp512a2 , cyp512v2 and cyp512a13 were upregulated in the VHb strain, suggesting that the increased ratio may be related to higher transcription levels of those cytochrome P450 genes.…”

Section: Resultsmentioning

confidence: 99%

“…Due to their important pharmacological activities, many strategies have been used to increase the production of GAs during mycelia fermentation. Progress has been achieved in the last several decades in enhancing GA production in G. lucidum by the manipulation of fermentation strategies (Xu et al ., ; Upadhyay et al ., ; Wang et al ., ), addition of metal ions and elicitors (Ren et al ., ; Xu and Zhong, ; You et al ., , ; Zhang et al ., ; Gu et al ., ) and genetic engineering (Xu et al ., ; Xu and Zhong, ; Li et al ., ,,; Zhang et al ., ,). However, further improvement of GA production is necessary to meet the demand of increasing preclinical studies and commercial applications.…”

Section: Introductionmentioning

confidence: 99%

“…reported that calcium ion addition improved GA production in a liquid static culture of G. lucidum (Xu and Zhong, ). Our recent work found that the heterologous expression of the Vitreoscilla haemoglobin (VHb) gene increased GA biosynthesis in submerged cultivation of G. lucidum (Li et al ., ). Therefore, in this study, we observed the effects of VHb expression and calcium ion induction on GA biosynthesis in a liquid static culture of G. lingzhi .…”

Section: Introductionmentioning

confidence: 99%

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Enhanced production of individual ganoderic acids by integrating Vitreoscilla haemoglobin expression and calcium ion induction in liquid static cultures of Ganoderma lingzhi

Yue

et al. 2019

Microbial Biotechnology

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Summary Ganoderic acids produced by Ganoderma exhibit anticancer and antimetastatic activities. A novel approach by combining Vitreoscilla haemoglobin (VHb) expression and calcium ion induction was developed to enhance ganoderic acid (GA) production in liquid static cultures of G. lingzhi. The maximum contents of GA‐O, GA‐S and GA‐Me were 1451.33 ± 67.50, 1431.23 ± 79.74 and 1283.81 ± 85.13 μg per 100 mg cell weight, respectively under the integrated approach, which are the highest contents as ever reported in Ganoderma. The contents of squalene and lanosterol were increased by 2.0‐ and 3.0‐fold in this case compared with those in the control. The transcription levels of 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase, farnesyl‐diphosphate synthase, squalene synthase and cytochrome P450 CYP5150L8 were upregulated by 2.56‐, 3.31‐, 2.59‐ and 6.12‐fold respectively. Additionally, the expression of VHb improved the ratio of type I to type II GA in liquid static cultivation of G. lingzhi. The transcription levels of cyp512a2, cyp512v2 and cyp512a13, candidate cytochrome P450 genes involved in oxidative modification of the lanostane skeleton in GA biosynthesis, were also increased by 2.28‐, 2.65‐ and 3.54‐fold in the VHb‐expressing strain respectively. Our results illustrated that the approach described here efficiently improved GA production in G. lingzhi fermentation.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enhanced production of individual ganoderic acids by integrating Vitreoscilla haemoglobin expression and calcium ion induction in liquid static cultures of Ganoderma lingzhi

Yue

et al. 2019

Microbial Biotechnology

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“…The static fermentation seemed to promote GAs yield higher, such as the GAs level increased 270% by Ca 2+ activation26, while Mn 2+ increased the GAs content by 220%27. In order to enhance the oxygen utilization of G.lucidum , mushroom transformed with Vitreoscilla hemoglobin gene showed up to two times higher GAs production over the non-transgenic control28. Nevertheless, the diverse treatments that brought to higher GAs production help to shape a better route towards the understanding of GAs synthesis and regulation.…”

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confidence: 99%

Improved ganoderic acids production in Ganoderma lucidum by wood decaying components

Ahmed

et al. 2017

Sci Rep

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Ganoderma lucidum is a legendary Traditional Chinese Medicine (TCM) over a few thousands of years and one kind of its major active components are Ganoderic acids (GAs). GAs are largely produced in the mushroom primordium and fruiting body but much less in mycelium stage. However, little is known on the underlying regulatory mechanism. As a saprophytic fungus, G. lucidum solely obtains nutrients by wood decaying. Wood in general contains sophisticated chemical components with diverse structural units. To explore a strategy that extensively leads to GAs induction in the submerged liquid fermentation, all chemical components that might be possibly from the wood decaying were tested individually as GAs inducers. It was found that GAs production increased 85.96% by 1.5% microcrystalline cellulose (MCC) and 63.90% by 0.5% D-galactose. The transcription level of a few rate-limiting or chemically diverting enzymes responsible for GAs biosynthesis was greatly induced by MCC and D-galactose. The concentration and time-course titration study indicated that these two chemicals might not be utilized as carbon sources but they played a comprehensive role in the secondary metabolites synthesis. Our data indicated that MCC and D-galactose might be further industrialized for higher GAs production in G. lucidum in submerged fermentation.

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Downstream Processing of Medicinal Mushroom Products

Luo

2022

Advances in Biochemical Engineering/Biotechnology

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Enhancement of ganoderic acid production by constitutively expressing Vitreoscilla hemoglobin gene in Ganoderma lucidum

Cited by 34 publications

References 38 publications

Enhanced production of individual ganoderic acids by integrating Vitreoscilla haemoglobin expression and calcium ion induction in liquid static cultures of Ganoderma lingzhi

Enhanced production of individual ganoderic acids by integrating Vitreoscilla haemoglobin expression and calcium ion induction in liquid static cultures of Ganoderma lingzhi

Improved ganoderic acids production in Ganoderma lucidum by wood decaying components

Downstream Processing of Medicinal Mushroom Products

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