Enhancement of Galactose Uptake from Kappaphycus alvarezii Hydrolysate Using Saccharomyces cerevisiae Through Overexpression of Leloir Pathway Genes

Sunwoo, In Yung; Sukwong, Pailin; Park, Yu Rim; Jeong, Deok Yeol; Kim, Soo Rin; Jeong, Gwi-Teak

doi:10.1007/s12010-020-03422-7

Cited by 9 publications

(2 citation statements)

References 30 publications

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“…The galE - galT - galK gene may have a positive effect on the UDP-Gal flux via the Leloir pathway. The intracellular availability of UDP-Gal promotes LNT biosynthesis. , …”

Section: Resultsmentioning

confidence: 99%

Engineering Escherichia coli for the High-Titer Biosynthesis of Lacto-N-tetraose

Miao

et al. 2022

J. Agric. Food Chem.

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Lacto-N-tetraose (LNT), a member of the human milk oligosaccharides family, has received widespread attention because of its importance in infant health. We constructed a whole-cell biotransformation method in Escherichia coli BL21(DE3) for high-titer LNT synthesis. The approach was performed by using a systematic design and metabolic engineering based on the metabolic pathway of LNT. The lgtA (encoding β-1,3-N-acetylglucosaminyltransferase) and wbgO (encoding β-1,3galactosyltransferase) genes were introduced into the engineered E. coli BL21(DE3) to construct an LNT-producing starting strain B1 (0.22 g/L). Then, the genes related to the LNT metabolic pathway were screened in two vectors to evaluate LNT synthesis. The lgtA-wbgO and galE-galT-galK genes were overexpressed through the two-plasmid system in E. coli BL21(DE3). The titer of LNT (3.42 g/L) had a gain of 14.55 times compared with that of B1. Furthermore, the ugd gene, which was associated with the UDP-Gal bypass pathway, was inactivated to further improve LNT production in shake-flask cultivation (4.14 g/L). The final fed-batch cultivation of the engineered strain produced 31.56 g/L of LNT. This study provided a strategy for the effective production of LNT in E. coli.

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“…The galE - galT - galK gene may have a positive effect on the UDP-Gal flux via the Leloir pathway. The intracellular availability of UDP-Gal promotes LNT biosynthesis. , …”

Section: Resultsmentioning

confidence: 99%

Engineering Escherichia coli for the High-Titer Biosynthesis of Lacto-N-tetraose

Miao

et al. 2022

J. Agric. Food Chem.

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“…Inhibitory compounds are released during the pretreatment processes [3], [5], [7]. Sunwoo et al indicated that reducing sugar compounds with extended treatment periods have been converted to inhibitory compounds [9] [6]. Factors that may be inhibiting the growth of S. cerevisiae are salts on the substrate.…”

Section: Introductionmentioning

confidence: 99%

Desalination Technique on Seaweeds Hydrolysate Eucheuma Cottonii for Bioethanol Production

Fakhrudin¹,

Setyaningsih²,

Rahayuningsih³

2021

Int. J. Eng. Scie. and Inform. Technology.

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The dissolved salts on Eucheuma cottonii hydrolysate interfere the growth of S. cerevisiae in the fermentation as it is considered as inhibitors. These salts are derived from biomass and formed from the chemicals used for hydrolysis processes such as H2SO4 and CaOH2. Ions and cations of the salts are potential as inhibitors such as Na+, Cl-, NH4+, SO42-. Osmotic pressure is raised due to the presence of salt. The efforts had been made to reduce salinity level through electrodialysis. The objective of this study was to eliminate dissolved salts in the hydrolysate in order to optimize the process of fermentation and increase ethanol yield. The results showed that the process of desalination by electrodialysis was able to reduce the salinity of 20% on the voltage of 5 V for 30 minutes; the voltage 9 V for 15 minutes; the voltage 12 V for 30 minutes. The lowest decline of SO42- occurred at treatment of 12 V for 45 minutes. The content of sulfate in the treatment decreased to 2.97 g/l or 46.22%. The more sulfate is reduced through the electrodialysis process, the better the fermentation process is carried out. Desalination treatment at a voltage of 5 V for 30 minutes had been the best treatment because it produced the highest ethanol yield of 2.06%. All the voltage treatments and the length of time on the electrodialysis process had a significant influence on the levels of ethanol yield. The process of desalination by electrodialysis was able to reduce the levels of dissolved salts, then it had a significant effect on ethanol yield.

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An overview of D-galactose utilization through microbial fermentation and enzyme-catalyzed conversion

Chen

et al. 2021

Appl Microbiol Biotechnol

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Enhancement of Galactose Uptake from Kappaphycus alvarezii Hydrolysate Using Saccharomyces cerevisiae Through Overexpression of Leloir Pathway Genes

Cited by 9 publications

References 30 publications

Engineering Escherichia coli for the High-Titer Biosynthesis of Lacto-N-tetraose

Engineering Escherichia coli for the High-Titer Biosynthesis of Lacto-N-tetraose

Desalination Technique on Seaweeds Hydrolysate Eucheuma Cottonii for Bioethanol Production

An overview of D-galactose utilization through microbial fermentation and enzyme-catalyzed conversion

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