Enhancement of fungichromin production of Streptomyces sp. WP-1 by genetic engineering

Wan, Miyang; Gan, Lu; Li, Zhenxin; Wang, Mengran; Chen, Jingtao; Chen, Shaoxin; Hu, Jin‐Feng; Li, Jiyang

doi:10.1007/s00253-023-12672-4

Cited by 2 publications

(1 citation statement)

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“…As the genetic background of strains changed after genetic engineering, it is necessary to optimize the medium or culture conditions to explore the production potential of engineered strains. This phenomenon has also been found in other strains(Li et al 2022 ; Wan et al 2023 ; Zhang et al 2023b ). RSM was widely used to design experiments and determine the optimal medium with fewer experimental trials and obtain more responses at the same time.…”

Section: Discussionsupporting

confidence: 79%

Enhancement of doxorubicin production in Streptomyces peucetius by genetic engineering and process optimization

Yang,

Gui,

Zhang

et al. 2024

AMB Expr

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Doxorubicin is an important class of anthracycline antitumor antibiotics produced by Streptomyces peucetius. The doxorubicin fermentation yield of the wild-type strain was very low, so it could not be produced directly by fermentation at an industrial scale due to the high cost. In the present study, S. peucetius SIPI-7-14 was obtained from SIPI-14 through several rounds of doxorubicin resistance screening. Then, the ketoreductase gene dnrU was knocked out to reduce (13S)-13-dihydrodaunorubicin production, and the resistance gene drrC was overexpressed to further enhance resistance to doxorubicin. The resulting engineered strain S. peucetius △U1/drrC produced 1128 mg/L doxorubicin, a 102.1% increase compared to that of SIPI-14. Then, fermentation medium was optimized using the response surface method. In the optimized fermentation medium, the yield of doxorubicin was increased to 1406 mg/L in shake flask on the 7th day. Furthermore, batch culture was carried out in a 10 L fermenter, and the concentration of doxorubicin reached 1461 mg/L after 7 days of culture, which was the highest yield reported to date, indicating the potential for industrial production of doxorubicin by fermentation.

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Section: Discussionsupporting

confidence: 79%