Enhancement of FITC Fluorescence by Silver Colloids and Silver Island Films

Kruszewski, Stefan; Wybranowski, Tomasz; Cyrankiewicz, Michał; Ziomkowska, Blanka; Pawlaczyk, Angelika

doi:10.12693/aphyspola.113.1599

Cited by 12 publications

(5 citation statements)

References 27 publications

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“…51 A critical energy transfer distance of 4.2 nm has been reported. 52 The same was observed for the core-cross-linked micelles, except that the fraction with the lower lifetime is higher for the cross-linked micelles. This result is coherent with the LS results showing a higher density of the core aer crosslinking.…”

Section: Fluorescence Lifetime: Flim Experimentssupporting

confidence: 58%

Complex coacervate core micelles as diffusional nanoprobes

2014

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Because of their ease of preparation and versatile modification opportunities, complex coacervate core micelles (C3Ms) may be a good alternative for expensive diffusional probes, such as dendrimers. However, C3Ms are unstable at high salt concentrations and may fall apart in contact with other polymers or (solid) materials. Therefore, we designed and characterized small (15 nm radius), stable fluorescent C3Ms. These were formed by electrostatic interactions between poly(ethylene oxide-methacrylic acid) (PEO-PMAA) and fluorescently labelled poly(allylamine hydrochloride) (PAH) and irreversible cross-linking of the core through amide bonds. We compared the properties of the cross-linked and non-cross-linked micelles. The radii of the two types of micelles were quite similar and independent of the ionic strength. Surprisingly, both were found to be stable at salt concentrations as high as 1.5 M. However, unlike the non-cross-linked C3Ms, the stability of the cross-linked C3Ms is independent of the pH. As a first example of their application as diffusional nanoprobes, we present results on the diffusion of the fluorescent micelles measured in xanthan solutions using fluorescence recovery after photobleaching (FRAP).

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Section: Fluorescence Lifetime: Flim Experimentssupporting

confidence: 58%

Complex coacervate core micelles as diffusional nanoprobes

2014

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“…Therefore to reduce the quenching effect and improve the luminescence efficiency of chromophores, an appropriate separation is required. Particular attention has been devoted recently to protein or DNA layers used as a spacer for the applications of MEF to biology [5,6]. In this work, a silver island film (SIF) is chosen to study plasmon enhanced luminescence.…”

Section: Introductionmentioning

confidence: 99%

Metal plasmon enhanced europium complex luminescence

Liu

Aldea

Nunzi

2010

Journal of Luminescence

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“…A variety of nanostructures have been proposed for the purpose such as silver nanoparticle (SNP), nanoshell, gold nanorod, silver nanotriangle, and silver island film (SIF) [ 25 – 40 ]. For example, SIF with discrete silver islands (SIs) on a substrate has been widely used for sensing single molecular fluorescence and protein in nano-biotechnology [ 2 , 5 , 8 , 10 ]. Recently, a hybrid nanostructure using SIF associated with SNPs has been developed to enhance the fluorescence of molecules located within the gap zone between the SI and SNP [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

Metal-Enhanced Fluorescence of Silver Island Associated with Silver Nanoparticle

Liaw

Huang

et al. 2016

Nanoscale Res Lett

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The coupling plasmon of a hybrid nanostructure, silver island (SI) associated with silver nanoparticle (SNP), on metal-enhanced fluorescence (MEF) was studied theoretically. We used the multiple multipole method to analyze the plasmon-mediated enhancement factor on the fluorescence of a molecule immobilized on SNP and located in the gap zone between SI and SNP; herein, the SI was modeled as an oblate spheroid. Numerical results show that the enhancement factor of the hybrid nanostructure is higher than that of a SNP or a SI alone due to the coupled gap mode. This finding is in agreement with the previous experimental results. In addition, the plasmon band of the structure is broadband and tunable, which can be red-shifted and broadened by flattening or enlarging SI. Based on this property, the hybrid nanostructure can be tailored to obtain the optimal enhancement factor on a specific molecule according to its excitation spectrum. Moreover, we found that there is an induced optical force allowing SNP be attracted by SI. Consequently, the gap is reduced gradually to perform a stronger MEF effect.

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Enhancement of FITC Fluorescence by Silver Colloids and Silver Island Films

Cited by 12 publications

References 27 publications

Complex coacervate core micelles as diffusional nanoprobes

Complex coacervate core micelles as diffusional nanoprobes

Metal plasmon enhanced europium complex luminescence

Metal-Enhanced Fluorescence of Silver Island Associated with Silver Nanoparticle

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