Enhancement of fingermarks and visualizing DNA

Kanokwongnuwut, Piyamas; Kirkbride, K. Paul; Kobus, Hilton; Linacre, Adrian

doi:10.1016/j.forsciint.2019.04.035

Cited by 28 publications

(13 citation statements)

References 21 publications

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“…For primary transfer, each participant was asked to rub their hands during around five seconds [13] with a view to redistribute surface DNA evenly on both of them [16], then took a knife (Stainless Steel, X50 Cr Mo V15) handle with their usual hand and, immediately after, stab three times a ballistic soap (from Mettler SA). 30 stabbing experiments were performed for each participant, leading in total to 180 experiments.…”

Section: Transfer Experimentsmentioning

confidence: 99%

Estimating the quantity of transferred DNA in primary and secondary transfers

2020

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Section: Transfer Experimentsmentioning

confidence: 99%

Estimating the quantity of transferred DNA in primary and secondary transfers

2020

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“…A number of previous studies have used different dye solutions using DD diluted with either 75% ethanol (EtOH) in sterile water for non-absorbent substrates such as glass slide, ziplock bag, and cartridge case [9,[13][14][15], or the dye diluted with water for staining DNA transferred to swabs, electric tape or PVC [16,17]. The comparison of the two different DD solutions on pre-enhanced fingermarks on non-absorbent substrates has been reported [18]. The application of DD in 75% EtOH in water was recommend, as it was shown to be less destructive to fingermark patterns than DD diluted with water [18].…”

Section: Introductionmentioning

confidence: 99%

“…The comparison of the two different DD solutions on pre-enhanced fingermarks on non-absorbent substrates has been reported [18]. The application of DD in 75% EtOH in water was recommend, as it was shown to be less destructive to fingermark patterns than DD diluted with water [18].…”

Section: Introductionmentioning

confidence: 99%

“…The comparison of the two different DD solutions on pre‐enhanced fingermarks on non‐absorbent substrates has been reported [18]. The application of DD in 75% EtOH in water was recommend, as it was shown to be less destructive to fingermark patterns than DD diluted with water [18]. A recent study recommended using DD diluted with 0.01% Triton‐X in water to stain latent DNA transferred onto tapes, as it was easier to spread the dye solution on the tape's sticky surface [19].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of a fluorescent dye to visualize touch DNA on various substrates

Champion

Kanokwongnuwut

Oorschot

et al. 2021

Journal of Forensic Sciences

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The ability to visualize the presence of cellular material deposited by touch onto items of forensic importance has the potential to greatly aid in criminal investigations. It is now routine in forensic casework to examine items for DNA assumed to have been deposited by touch [1-3]. Such DNA is often referred to as 'touch DNA' even though samples collected from such deposits can also include DNA from undetermined biological sources other than that derived from skin and/or background DNA from actions other than touching [4]. Visualizing the location of cellular material, and therefore the source of DNA, will allow examiners to target sampling to areas where DNA is present, and not sample areas where DNA is absent.Without knowledge of cellular material location, collection of DNA,

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“…Previous studies have investigated various fingerprint development techniques for porous [4][5][6][7][8] and non-porous [9][10][11][12][13][14][15][16] substrates and their subsequent effect on DNA analysis. Adverse effects that can occur include DNA contamination, introduction of inhibitors that prevent DNA analysis or degradation of DNA.…”

Section: Introductionmentioning

confidence: 99%

The compatibility of immunolabeling with STR profiling

Achetib

Weert

Birkl

et al. 2021

Forensic Science International: Genetics

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Immunolabeling is a technique, which has recently been introduced to enhance the quality of developed fingermarks and subsequently strengthen the evidential value. The effect of this method on subsequent DNA analysis, however, has not been explored yet. Therefore, the current pilot study aimed to determine whether STR profiling is possible after immunolabeling. Since immunolabeling involves washing steps which could reduce DNA quantities, the use of different fixatives including methanol, formaldehyde and universal molecular fixative (UMFIX) were investigated. STR profiles from the (immunolabeled) fingermarks were generated after four days and four weeks by a direct PCR method to enable comparison of relatively fresh and old fingermarks. The fingermarks were deposited on diverse forensically relevant substrates, including glass, metal and tile. STR profiles could be recovered for all tested fixatives with no significant difference in performance. However, the mean number of detected alleles was the highest when methanol was used for fixation. Furthermore, immunolabeling on aged fingermarks (4 weeks) was also possible, but the number of detected alleles showed a non-significant decrease. DNA could be recovered from deposits on all substrates, of which glass showed the highest mean number of detected alleles followed by metal and tile.

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Enhancement of fingermarks and visualizing DNA

Cited by 28 publications

References 21 publications

Estimating the quantity of transferred DNA in primary and secondary transfers

Estimating the quantity of transferred DNA in primary and secondary transfers

Evaluation of a fluorescent dye to visualize touch DNA on various substrates

The compatibility of immunolabeling with STR profiling

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