Enhancement of fibronectin expression in rat lung by ozone and an inflammatory stimulus

Abstract

This study investigated the relationship of fibronectin expression and induction of pulmonary inflammation by ozone (O3). Rats were exposed to 0.8 parts/million O3 to induce lung inflammation. A second inflammatory stimulus, rabbit serum, was applied intratracheally to augment O3-induced inflammation. Bronchoalveolar lavage fluid (BALF) and lung tissues were analyzed for fibronectin protein and mRNA expression. Blood plasma was analyzed to investigate the potential of a minimally invasive procedure in predicting lung inflammation and fibronectin levels. Significant increases in the levels of fibronectin protein in the BALF and lung tissue after O3 exposure were further enhanced by pretreatment with normal serum. An increase in fibronectin mRNA following O3 exposure was also enhanced by serum pretreatment, which by itself had no effect on lung fibronectin mRNA expression. Plasma fibronectin levels were comparable in air-PBS and O3-PBS groups but increased in the O3-serum group. The results suggest leakage of fibronectin from blood plasma into the lung following intratracheal application of rabbit serum and upregulation of local synthesis following O3 exposure.

Evaluation of ozone for preventing fungal influenced corrosion of reinforced concrete bridges over the River Nile, Egypt

“…Non significant increase in nucleic acids contents was observed at the lowest ozone exposure times (0-40 min) ( Table 3). The obtained result was in accordance with Gupta et al (2010) who stated that there are a significant increases in the levels of protein, RNA following ozone exposure. Also Poli et al (2007) found that with longer duration of ozone exposure (up to 30 min) cell viability decreased and membrane permeability was compromised as indicated by more significant increase in protein and nucleic acid leakage and lipid oxidation.…”

Fibronectin FacilitatesMycobacterium tuberculosisAttachment to Murine Alveolar Macrophages

“…Therefore, Fn may act as a bridge between M. tuberculosis and the AM. As Fn is a constitutively expressed protein in the lung (7) and is increased during inflammation (16), this interaction may represent a normal host response in the initial week of new infection in the alveolar spaces.…”