Enhancement of expression of survivin promoter-driven CD/TK double suicide genes by the nuclear matrix attachment region in transgenic gastric cancer cells

Niu, Yingjie; Li, Jiansheng; Luo, Xian-Run

doi:10.1016/j.gene.2013.10.064

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…The expression levels of the genes were determined by a threshold cycle number (Ct), which was normalized against the internal reference gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) by using the ΔΔCt method. The primer pairs are listed as follows [ 14 , 18 ]:…”

Section: Methodsmentioning

confidence: 99%

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

Chang

Ling

et al. 2021

Bioengineered

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Differentiated thyroid cancer (DTC), such as papillary thyroid cancer, has a good prognosis after routine treatment. However, in the course of treatment, 5% to 20% of cases may dedifferentiate and can be transformed into dedifferentiated DTC (deDTC) or anaplastic thyroid cancer, leading to treatment failure. To date, several drugs have been used effectively for dedifferentiated thyroid cancer, whereas gene therapy may be a potential method. Literature reported that double suicide genes driven by human telomerase reverse transcriptase promoter (hTERTp) can specifically express in cancer cells and kill them. However, the weak activity of hTERTp limits its further research. To overcome this weakness, we constructed a novel chitosan nanocarrier containing double suicide genes driven by a 'gene switch' (a cascade of radiation enhancer E9 and a hTERTp). The vector was labeled with iodine-131 ( 131 I). On one hand, E9 can significantly enhance the activity of hTERTp under the weak radiation of 131 I, thereby increasing the expression of double suicide genes in deDTC cells. On the other hand, 131 I also plays a certain killing role when it enters host cells. The proposed nanocarrier has good specificity for deDTC cells and thus deserves further study.

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Section: Methodsmentioning

confidence: 99%

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

Chang

Ling

et al. 2021

Bioengineered

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“…[21] Several studies use both systems, HSV-TK/GCV and CD/5-FC, and have been shown to support a synergistic interaction. [22][23][24] Huang et al [25] , designed a double suicide gene system with CD/TK to study their bystander effects. Colon cancer cells were transfected with this double system and, after transfected, GCV or 5-FU prodrugs or both of them were administered.…”

Section: Introductionmentioning

confidence: 99%

On Advances in Cancer Suicide-genes Therapy

Rama¹

2014

IJGS

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“…The 'by-stander effect' increases the cytotoxicity against target cells 11 . The anticancer potential of CD-producing MSCs has been demonstrated in a broad spectrum of solid cancers 11,12 , including gastric cancer 13,14 , breast cancer 15,16 , and glioblastoma 17,18 . Preclinical studies have demonstrated that cytosine deaminase/5-fluocytosine (CD/5FC) is highly robust, where as low as 4% of CD positive cells in the tumour mass is sufficient to completely eradicate the tumour 19 .…”

mentioning

confidence: 99%

A highly efficient non-viral process for programming mesenchymal stem cells for gene directed enzyme prodrug cancer therapy

Woo

et al. 2020

Sci Rep

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Mesenchymal stem cells (MSCs) driven gene-directed enzyme prodrug therapy has emerged as a potential strategy for cancer treatment. The tumour-nesting properties of MSCs enable these vehicles to target tumours and metastases with effective therapies. A crucial step in engineering MSCs is the delivery of genetic material with low toxicity and high efficiency. Due to the low efficiency of current transfection methods, viral vectors are used widely to modify MSCs in preclinical and clinical studies. We show, for the first time, the high transfection efficiency (> 80%) of human adipose tissue derived-MSCs (AT-MSCs) using a cost-effective and off-the-shelf Polyethylenimine, in the presence of histone deacetylase 6 inhibitor and fusogenic lipids. Notably, the phenotypes of MSCs remained unchanged post-modification. AT-MSCs engineered with a fused transgene, yeast cytosine deaminase::uracil phosphoribosyltransferase (CDy::UPRT) displayed potent cytotoxic effects against breast, glioma, gastric cancer cells in vitro. The efficiency of eliminating gastric cell lines were effective even when using 7-day post-transfected AT-MSCs, indicative of the sustained expression and function of the therapeutic gene. In addition, significant inhibition of temozolomide resistant glioma tumour growth in vivo was observed with a single dose of therapeutic MSC. This study demonstrated an efficient nonviral modification process for MSC-based prodrug therapy.

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Enhancement of expression of survivin promoter-driven CD/TK double suicide genes by the nuclear matrix attachment region in transgenic gastric cancer cells

Cited by 6 publications

References 22 publications

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

Enhancement of nanoparticle-mediated double suicide gene expression driven by ‘E9-hTERT promoter’ switch in dedifferentiated thyroid cancer cells

On Advances in Cancer Suicide-genes Therapy

A highly efficient non-viral process for programming mesenchymal stem cells for gene directed enzyme prodrug cancer therapy

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