Enhancement of exopolysaccharide production from Ganoderma lucidum using a novel submerged volatile co-culture system

Asadi, Fatemeh; Barshan-Tashnizi, Mohammad; Hatamian-Zarmi, Ashrafalsadat; Davoodi-Dehaghani, Fatemeh; Ebrahimi-Hosseinzadeh, Bahman

doi:10.1016/j.funbio.2020.09.010

Cited by 15 publications

(10 citation statements)

References 46 publications

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“…Polysaccharides from edible and medicinal fungi have a variety of biological functions, such as anti-tumor, antiviral, antioxidant, and immunomodulatory properties [4][5][6]. In recent years, the method for obtaining polysaccharides produced by such fungi through submerged fermentation has become more sophisticated [28,29]. Optimization of culture condition and medium composition has shown to be essential for promoting adequate mycelial growth and production of polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

Anthocyanin extract from Lycium ruthenicum enhanced production of biomass and polysaccharides during submerged fermentation of Agaricus bitorquis (Quél.) Sacc. Chaidam

Chen

et al. 2021

Bioprocess Biosyst Eng

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Agaricus bitorquis (Quél.) Sacc. Chaidam (ABSC) is a wild edible fungus uniquely found in the Tibet Plateau. ABSC is rich in polysaccharides that are considered biologically active. This study aimed to determine the feasibility of enhancing exopolysaccharide (EPS) production by ABSC in shake flask culture by supplementing the fermentation medium with anthocyanin extract. Different concentrations of Lycium ruthenicum Murr. (LRM) anthocyanin crude extract were tested on ABSC fermentation. The activity of phosphoglucose isomerase (PGI), phosphoglucose mutase (PGM), and phosphomannose isomerase (PMI), enzymes presumably involved in EPS synthesis by ABSC, was determined. ABSC transcriptomic profile in response to the presence of anthocyanins during fermentation was also investigated. LRM anthocyanin crude extract (0.06 mg/mL) was most effective in increasing EPS content and mycelial biomass (by 208.10% and 105.30%, respectively, P < 0.01). The activity of PGI, PGM, and PMI was increased in a medium where LRM anthocyanin extract and its main components (proanthocyanidins and petunia anthocyanin) were added. RNA-Seq analysis showed that 349 genes of ABSC were differentially expressed during fermentation in the medium containing anthocyanin extract of LRM; 93 genes were up-regulated and 256 genes down-regulated. From gene ontology enrichment analysis, differentially expressed genes were mostly assigned to carbohydrate metabolism and signal transduction categories. Collectively, LRM anthocyanins extract positively affected EPS production and mycelial biomass during ABSC fermentation. Our study provides a novel strategy for improving EPS production and mycelial growth during ABSC liquid submerged fermentation.

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Section: Discussionmentioning

confidence: 99%

Anthocyanin extract from Lycium ruthenicum enhanced production of biomass and polysaccharides during submerged fermentation of Agaricus bitorquis (Quél.) Sacc. Chaidam

Chen

et al. 2021

Bioprocess Biosyst Eng

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“…After culture for 3 days, 1.0 g biofilms formed by test strains were picked for the determination of EPS content. The biofilms were mixed with 200 μl sterile water and shaken thoroughly to disperse all the aggregates, and then the EPS content in the supernatant of heavy suspension was determined according to previous report ( Asadi et al, 2020 ) with some modifications. For detail, equal volume of 1 M NaOH was added to the supernatant and incubated at 60°C for 1 h. The mixture was centrifuged (10 min and12,000 rpm), and the polysaccharides content in the supernatant was determined by the phenol-sulfuric acid method.…”

Section: Methodsmentioning

confidence: 99%

GapB Is Involved in Biofilm Formation Dependent on LrgAB but Not the SinI/R System in Bacillus cereus 0-9

Zhang

et al. 2020

Front. Microbiol.

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Bacillus cereus 0-9, a Gram-positive endospore-forming bacterium isolated from healthy wheat roots, has biological control capacity against several soil-borne plant diseases of wheat such as sharp eyespot and take-all. The bacterium can produce various biofilms that differ in their architecture and formation mechanisms, possibly for adapting to different environments. The gapB gene, encoding a glyceraldehyde-3-phosphate dehydrogenase (GAPDH), plays a key role in B. cereus 0-9 biofilm formation. We studied the function of GapB and the mechanism of its involvement in regulating B. cereus 0-9 biofilm formation. GapB has GAPDH activities for both NAD+- and NADP+-dependent dehydrogenases and is a key enzyme in gluconeogenesis. Biofilm yield of the ΔgapB strain decreased by 78.5% compared with that of wild-type B. cereus 0-9 in lysogeny broth supplemented with some mineral salts (LBS), and the ΔgapB::gapB mutants were recovered with gapB gene supplementation. Interestingly, supplementing the LBS medium with 0.1–0.5% glycerol restored the biofilm formation capacity of the ΔgapB mutants. Therefore, GapB regulates biofilm formation relative to its function in gluconeogenesis. To illustrate how GapB is involved in regulating biofilm formation through gluconeogenesis, we carried out further research. The results indicate that the GapB regulated the B. cereus 0-9 biofilm formation independently of the exopolysaccharides and regulatory proteins in the typical SinI/R system, likely owing to the release of extracellular DNA in the matrix. Transcriptome analysis showed that the gapB deletion caused changes in the expression levels of only 18 genes, among which, lrgAB was the most significantly increased by 6.17-fold. We confirmed this hypothesis by counting the dead and living cells in the biofilms and found the number of living cells in the biofilm formed by the ΔgapB strain was nearly 7.5 times than that of wild-type B. cereus 0-9. Therefore, we concluded that the GapB is involved in the extracellular DNA release and biofilm formation by regulating the expression or activities of LrgAB. These results provide a new insight into the regulatory mechanism of bacterial biofilm formation and a new foundation for further studying the stress resistance of B. cereus.

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“…Fungal EPSs comprise monomeric units such as D-xylose, fucose, etc. The fungal EPSs are diverse in the monosaccharide linkage patterns, monosaccharide units, molecular mass, branching degree, glycosidic bond, and conformation (Asadi et al, 2021;Gong et al, 2020). In addition, EPSs comprised of similar monosaccharide entities produced by various fungi had distinct molecular weights (MWs) (Elsehemy et al, 2020).…”

Section: General Properties Of Fungal Epssmentioning

confidence: 99%

“…fungal EPSs production and their potential for biomedical applications such as anti-inflammatory effects (Barbieri et al, 2017;Rajasekar, Selvakumar, Periasamy, & Raaman, 2008), improvement of the immune system (Rajasekar et al, 2008;Zhao, Chen, & He, 2018), anticancer actions (Barbieri et al, 2017;S. Mahapatra & D. Banerjee, 2013;Rajasekar et al, 2008;Zhao et al, 2018) influence on the cardiovascular system (S. Mahapatra & D. Banerjee, 2013;Rajasekar et al, 2008), and treatment of hypercholesterolemia and diabetes (Asadi, Barshan-Tashnizi, Hatamian-Zarmi, Davoodi-Dehaghani, & Ebrahimi-Hosseinzadeh, 2021;S. Mahapatra & D. Banerjee, 2013;Rajasekar et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Fungal exopolysaccharides: Properties, sources, modifications, and biomedical applications

Hamidi

Okoro

Milan

et al. 2022

Carbohydrate Polymers

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Enhancement of exopolysaccharide production from Ganoderma lucidum using a novel submerged volatile co-culture system

Cited by 15 publications

References 46 publications

Anthocyanin extract from Lycium ruthenicum enhanced production of biomass and polysaccharides during submerged fermentation of Agaricus bitorquis (Quél.) Sacc. Chaidam

Anthocyanin extract from Lycium ruthenicum enhanced production of biomass and polysaccharides during submerged fermentation of Agaricus bitorquis (Quél.) Sacc. Chaidam

GapB Is Involved in Biofilm Formation Dependent on LrgAB but Not the SinI/R System in Bacillus cereus 0-9

Fungal exopolysaccharides: Properties, sources, modifications, and biomedical applications

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