Enhancement of Embryonic Stem Cell Differentiation Promoted by Avian Chorioallantoic Membranes

Talavera-Adame, Dodanim; Dafoe, Donald C.; Ng, Tina T.; Wachsmann‐Hogiu, Sebastian; Castillo-Henkel, Carlos; Farkas, Daniel L.

doi:10.1089/ten.tea.2009.0024

Cited by 10 publications

(12 citation statements)

References 25 publications

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“…Our results support this hypothesis since scarce blood circulation was observed in the blood vessels yet the differentiation enhancement took place. In addition, we reported enhancement of differentiation using mouse EBs implanted in surrogate vasculature from quail embryos [7]. These findings also support the idea of EC-derived factors as mediators of the differentiation process.…”

supporting

confidence: 84%

“…With the emergence of embryonic stem cells (ESCs), studies to investigate the role of ECs in organogenesis can now be performed in vitro. We previously described an approach to investigate the inductive effects of ECs in cell differentiation by implanting embryoid bodies (EBs) into surrogate vascular beds, such as quail chorioallantoic membranes [7]. Recently, we also reported the enhancement of pancreatic progenitors and insulin-producing cells in EBs cocultured with human microvascular endothelial cells (HMECs) [8].…”

Section: Introductionmentioning

confidence: 99%

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Bone Morphogenetic Protein-2/-4 Upregulation Promoted by Endothelial Cells in Coculture Enhances Mouse Embryoid Body Differentiation

Talavera-Adame

Gupta²,

Kurtovic³

et al. 2013

Stem Cells and Development

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Endothelial cells (ECs) provide inductive signals for cell differentiation in vivo. However, it is unknown if these cells promote such differentiation in vitro and the signals involved. We investigated whether ECs are able to enhance the differentiation of the three germ layers and the underlying mechanisms. We established a coculture system of mouse embryoid bodies (EBs) and ECs. Then, we analyzed the expression of markers representative of the three germ layers, such as PDX-1, proinsulin, insulin1 (endoderm), nestin, neurofilament light (ectoderm), CD31, cardiotin, and cardiac troponin I (mesoderm) in EBs cultured alone (controls) or with ECs. A significant increase of these markers was observed in EBs cocultured with ECs compared to controls. The cocultured EBs also exhibited more robust vascular networks similar to those EBs treated with bone morphogenetic protein-2 or -4 (BMP-2 or -4). Therefore, the role of these peptides in the differentiation was investigated. We found a significant upregulation of BMP-2/-4 and BMP receptor 1A in EBs treated with EC conditioned medium (EC-CM) at early or middle stages of EB development. Recombinant human BMP-2 and BMP-4 exerted similar effects than EC-CM in the expression of BMPs or in the upregulation of the three germ layer specific markers. BMP-2/-4 antagonists, such as noggin and chordinlike-1, respectively inhibited the EC-CM inductive effects. These results demonstrate that ECs enhance the differentiation in vitro of cells that derived from the three germ layers and that BMP-2/-4 play a central role in this process.

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supporting

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Bone Morphogenetic Protein-2/-4 Upregulation Promoted by Endothelial Cells in Coculture Enhances Mouse Embryoid Body Differentiation

Talavera-Adame

Gupta²,

Kurtovic³

et al. 2013

Stem Cells and Development

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“…The blood capillaries were found to grow radially towards the hDPSC-containing matrigel droplets. These findings suggest that the secretion of angiogenic molecules is responsible for the hDPSC-induced angiogenesis in the CAM, as the applied incubation period of 72 hours is considered to be too short to induce EC transdifferentiation of the transplanted cells [45], [55], [56]. Undifferentiated and endothelial-differentiated placental MSC also have angiogenic activities in this in vivo -like animal model [55].…”

Section: Discussionmentioning

confidence: 90%

Angiogenic Properties of Human Dental Pulp Stem Cells

et al. 2013

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Angiogenesis, the formation of capillaries from pre-existing blood vessels, is a key process in tissue engineering. If blood supply cannot be established rapidly, there is insufficient oxygen and nutrient transport and necrosis of the implanted tissue will occur. Recent studies indicate that the human dental pulp contains precursor cells, named dental pulp stem cells (hDPSC) that show self-renewal and multilineage differentiation capacity. Since these cells can be easily isolated, cultured and cryopreserved, they represent an attractive stem cell source for tissue engineering. Until now, only little is known about the angiogenic abilities and mechanisms of the hDPSC. In this study, the angiogenic profile of both cell lysates and conditioned medium of hDPSC was determined by means of an antibody array. Numerous pro-and anti-angiogenic factors such as vascular endothelial growth factor (VEGF), monocyte chemotactic protein-1 (MCP-1), plasminogen activator inhibitor-1 (PAI-1) and endostatin were found both at the mRNA and protein level. hDPSC had no influence on the proliferation of the human microvascular endothelial cells (HMEC-1), but were able to significantly induce HMEC-1 migration in vitro. Addition of the PI3K-inhibitor LY294002 and the MEK-inhibitor U0126 to the HMEC-1 inhibited this effect, suggesting that both Akt and ERK pathways are involved in hDPSC-mediated HMEC-1 migration. Antibodies against VEGF also abolished the chemotactic actions of hDPSC. Furthermore, in the chicken chorioallantoic membrane (CAM) assay, hDPSC were able to significantly induce blood vessel formation. In conclusion, hDPSC have the ability to induce angiogenesis, meaning that this stem cell population has a great clinical potential, not only for tissue engineering but also for the treatment of chronic wounds, stroke and myocardial infarctions.

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“…We found that some cells expressed cardiotin, myosin heavy chain, collagen Ⅳ, CD34, CD31, and neurofilament. Although some epithelial cells appeared, no cells derived from endoderm were identified [90] . Then, studies using co-cultures between human microvascular ECs (HMECs) and mouse EBs were performed [52,53,91] .…”

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

“…As a first approach to investigate the role of endothelium in b-cell differentiation, we studied the role of in vivo surrogate vasculature in mouse embryoid body (EB) differentiation using quail chorioallantoic membranes (CAM) [90] . We found that some cells expressed cardiotin, myosin heavy chain, collagen Ⅳ, CD34, CD31, and neurofilament.…”

Section: Endothelium and Beta-cell Regeneration In Vivo And In Vitromentioning

confidence: 99%

Endothelium-derived essential signals involved in pancreas organogenesis

Talavera-Adame

Dafoe

2015

WJEM

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Endothelial cells (ECs) are essential for pancreas differentiation, endocrine specification, and endocrine function. They are also involved in the physiopathology of type 1 and type 2 diabetes. During embryogenesis, aortic ECs provide specific factors that maintain the expression of key genes for pancreas development such as pancreatic and duodenal homeobox-1. Other unknown factors are also important for pancreatic endocrine specification and formation of insulin-producing beta cells. Endocrine precursors proliferate interspersed with ductal cells and exocrine precursors and, at some point of development, these endocrine precursors migrate to pancreatic mesenchyme and start forming the islets of Langerhans. By the end of the gestation and close to birth, these islets contain immature beta cells with the capacity to express vascular endothelial growth factor and therefore to recruit ECs from the surrounding microenvironment. ECs in turn produce factors that are essential to maintain insulin secretion in pancreatic beta cells. Once assembled, a cross talk between endocrine cells and ECs maintain the integrity of islets toward an adequate function during the whole life of the adult individual. This review will focus in the EC role in the differentiation and maturation of pancreatic beta cells during embryogenesis as well as the current knowledge about the involvement of endothelium to derive pancreatic beta cells in vitro from mouse or human pluripotent stem cells.

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Enhancement of Embryonic Stem Cell Differentiation Promoted by Avian Chorioallantoic Membranes

Cited by 10 publications

References 25 publications

Bone Morphogenetic Protein-2/-4 Upregulation Promoted by Endothelial Cells in Coculture Enhances Mouse Embryoid Body Differentiation

Bone Morphogenetic Protein-2/-4 Upregulation Promoted by Endothelial Cells in Coculture Enhances Mouse Embryoid Body Differentiation

Angiogenic Properties of Human Dental Pulp Stem Cells

Endothelium-derived essential signals involved in pancreas organogenesis

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