Enhancement of DNA micro-array analysis using a shear-driven micro-channel flow system

Peer Reviewed: Shear-Driven Flow Approaches to LC and Macromolecular Separations

“…Another application is DNA microarray screening (Figure 4b), which is usually performed in a purely diffusion-driven mode overnight to obtain a detectable signal. By putting the DNA samples in SDF channels and forcing them to pass each spot of the array under a high degree of spatial confinement, we achieved a drastic increase (up to 30ϫ faster) in hybridization rates (33). Currently, we are investigating the practical utility of several different flowchamber layouts.…”

Exploiting the benefits of miniaturization for the enhancement of DNA microarrays

“…The methods proposed include surface acoustic wave (SAW)-based microagitation [4], cavitation microstreaming [5], electronical assistance [6], pressure-and electrically-driven flow generation [7][8][9], the use of pump-around systems [10], chaotic mixing [11], sample oscillation [12], shear-driven flow systems [13], etc. Central to all these approaches is that they use some form of mechanical or electrical forces to increase the transport rate of the sample DNA strands beyond their normal (extremely slow) diffusion transport rate.…”

A novel microstep device for the size separation of cells

“…This so-called shear-driven flow principle can be used to enhance the speed and resolution of a variety of microfluidics applications such as on-chip liquid chromatography (LC) [22][23][24], DNA microarray screening [25], and target-receptor binding kinetics measurements. The major advantage of shear-driven flows is that the applicable fluid velocity is independent of the fluid viscosity and the channel depth and length, hence completely avoiding the limitations on the applicable flow velocities existing in pressure-and electrokinetically driven nanochannels.…”