Enhancement of Diosgenin Production in Plantlet and Cell Cultures of &lt;i&gt;Dioscorea zingiberensis&lt;/i&gt; by Palmarumycin C&lt;sub&gt;13&lt;/sub&gt; from the Endophytic fungus, &lt;i&gt;Berkleasmium &lt;/i&gt;sp. Dzf12

Mou, Yan; Zhou, Kai; Xu, Dan; Rong, Yu; Li, J; Yin, None Chunhui; Zhou, Ligang

doi:10.4314/tjpr.v14i2.8

Cited by 4 publications

(2 citation statements)

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“…In present study, it was found that the expressions of DzDXR did not match the amounts of diosgenin in the leaves and tubers of D. zingiberensis at all. But this does not mean that the MEP pathway is not involved in diosgenin biosynthesis in Dioscorea because secondary metabolites (intermediates and end products) can be transported from the synthesis sites to the other sites (Nagata et al 2002, Burlat et al 2004, Li and Hu 2009. Bennett et al (1963) found that the shoot of D. spiculiflora was the major site of synthesis of phytosterols and sapogenins and believed that they were rapidly translocated to the tuber.…”

Section: Discussionmentioning

confidence: 99%

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Cloning, characterization, and expression of 1-deoxy-D-xylulose-5-phosphate reductoisomerase gene from Dioscorea zingiberensis

Cheng¹,

Wang²,

Shen³

et al. 2019

Biol. plant.

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Diosgenin, mainly extracted from some Dioscorea species, is the most important starting material for the production of steroidal drugs. It is believed that diosgenin in Dioscorea is synthesized from isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP, the isomer of IPP) produced by the cytosolic mevalonate pathway. So far, the possibility of the plastidic 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for biosynthesis of diosgenin remains unclear. The key enzyme in the MEP pathway is 1-deoxy-D-xylulose 5-phosphate-reductoisomerase (DXR, EC: 1.1.1.267). In this study, a DXR gene, named DzDXR (GenBank accession number KY131955), was isolated from Dioscorea zingiberensis. The DzDXR has an open reading frame of 1 413 bp encoding a protein of 470 amino acid residues. The function of DzDXR was verified by a colour enhancement assay in the Escherichia coli cells harbouring the plasmid pAC-BETA. Bioinformatic analyses revealed that DzDXR had a putative plastid transit peptide at the N-terminal region and was highly homologous to other plant DXRs, especially to those in monocotyledons. During the growth period of D. zingiberensis, the expression of DzDXR was found significantly high in leaves and very low in tubers, and the highest expression was observed in mature leaves in summer. In contrast to the DzDXR transcription, diosgenin was present predominantly in tubers and in minute quantities in leaves. Because diosgenin is very likely formed mainly in the cytosol of mature leaf cells of Dioscorea and the plastidic IPP and DMAPP produced by the MEP pathway can be transported into the cytosol, the consistently high expression of DzDXR detected in mature leaf of D. zingiberensis implies that the MEP pathway might play a significant role in diosgenin biosynthesis.

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Section: Discussionmentioning

confidence: 99%

“…All the samples were dried at 60 °C and then grounded to powder. Diosgenin extraction was done as described by Mou et al (2015) with some modifications. Briefly, about 100 mg of the powder was added into a 50 cm 3 flask containing 20 cm 3 of 1 M sulfuric acid and kept at 125 °C for 3 h for hydrolysis.…”

Section: Diosgenin Determinationmentioning

confidence: 99%