Enhancement of chromatin availability and RNA polymerase activity in Cicer arietinum shoots by cAMP

Grover, Anil; Kapoor, H. C.

doi:10.1016/0031-9422(89)80096-2

Cited by 2 publications

(1 citation statement)

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“…Notably, a previous study showed that increased RNA polymerase activity of the influenza virus enhances the activation of the HA-induced Raf/MEK/ERK signaling cascade [ 47 ]. Other studies have reported a link between cAMP and RNA polymerase in chickpeas [ 48 , 49 ]. Therefore, we suggest that the link between cAMP levels and viral RNA polymerase activity in mammalian cells may explain the effects of FSK on influenza virus propagation.…”

Section: Discussionmentioning

confidence: 99%

The Adenylyl Cyclase Activator Forskolin Increases Influenza Virus Propagation in MDCK Cells by Regulating ERK1/2 Activity

Lee,

Park

et al. 2023

J. Microbiol. Biotechnol.

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Vaccination is the most effective method for preventing the spread of the influenza virus. Cell-based influenza vaccines have been developed to overcome the disadvantages of egg-based vaccines and their production efficiency has been previously discussed. In this study, we investigated whether treatment with forskolin (FSK), an adenylyl cyclase activator, affected the output of a cell-based influenza vaccine. We found that FSK increased the propagation of three influenza virus subtypes (A/H1N1/California/4/09, A/H3N2/Mississippi/1/85, and B/Shandong/7/97) in Madin–Darby canine kidney (MDCK) cells. Interestingly, FSK suppressed the growth of MDCK cells. This effect could be a result of protein kinase A (PKA)–Src axis activation, which downregulates extracellular signal-regulated kinase (ERK)1/2 activity and delays cell cycle progression from G 1 to S. This delay in cell growth might benefit the binding and entry of the influenza virus in the early stages of viral replication. In contrast, FSK dramatically upregulated ERK1/2 activity via the cAMP–PKA–Raf-1 axis at a late stage of viral replication. Thus, increased ERK1/2 activity might contribute to increased viral ribonucleoprotein export and influenza virus propagation. The increase in viral titer induced by FSK could be explained by the action of cAMP in assisting the entry and binding of the influenza virus. Therefore, FSK addition to cell culture systems could help increase the production efficiency of cell-based vaccines against the influenza virus.

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Section: Discussionmentioning

confidence: 99%