Enhancement of Chondrogenesis in Hypoxic Precondition Culture: A Systematic Review

Abstract

BACKGROUND: Cartilage tear has begun to be treated with stem cells. However, stem cell oxygen level culture has not been evaluated for the best environment to enhance chondrogenesis. AIM: The purpose of this review is to focus on the hypoxic oxygen level of stem cells culture as a treatment for cartilage tear. METHODS: A literature search was systemically conducted on PubMed (MEDLINE), OVID, EMBASE, the Cochrane Library, Scopus, Web of Science, Science Direct, Wiley Online Library, Google Scholar, and bibliography of selected articles with the terms (“culture”) AND (“stem cell” OR “mesenchymal stem cell” OR “MSC”) AND (“hypoxic” OR “hypoxia”) AND (“cartilage” OR “chondro*”) as the main keywords. A total of 438 articles were reviewed. Thirty-six articles were considered relevant for this systematic review. RESULTS: The result of this review supports stimulation effects of hypoxic oxygen level stem cell culture in chondrogenesis process. Most studies used 5% oxygen concentration for culture, both of in vivo and in vitro studies. Due to the heterogeneity nature of the included studies, meta-analysis was unable to be conducted. CONCLUSION: Hypoxia state seems to play an important role in chondrocytes proliferation, differentiation, and matrix production.

Chondroregenerative Potential of Platelet-Rich Fibrin (PRF)-Impregnated Decelulrized Bovine Cartilage Scaffold Implanted Subcutaneously

“…Research have not yet reported significant differences in the use of animal-derived or synthetic biomaterials. Several lines of evidence suggest that synthetic materials have a lower risk of disease transmission (Barakat et al 2008, Rhatomy et al 2021. Deproteinization is an essential process to eliminate the antigenicity of xenograft bone.…”

“…In the future, the BCPRF can be developed as an alternative implant capable of replacing the use of autologous cartilage. Rhatomy et al (2021) stated that decellularized biomaterial scaffold limits the use of growth factor, resulting in better cost and resource efficiency. Organic materials are preferred because they have better biocompatibility and biodegradability than synthetic materials (Park & Cho 2010).…”

Critical appraisal tools used in systematic reviews of in vitro cell culture studies: A methodological study

“…T A B L E 3 Tools and instruments used in the included studies (n = 82). ,59,[65][66][67]69,71,73,74,76,77,[79][80][81]83,84,87,89,[91][92][93][94]96,98,100,102,107,[109][110][111][112]114,115,[118][119][120][121][122][123][124][125][127][128][129][130][131][132]136 : CONSORT, Consolidated Standards of Reporting Trials; GRADE, Grading of Recommendations Assessment, Development and Evaluation; OHAT, Oral Health Assessment Tool; SYRCLE, Systematic Review Centre for Laboratory Animal Experimentation; ToxRTool, Toxicological data Reliability Assessment Tool.…”