Enhancement of cellulase catalysis of wood pulp fiber by cationic polyelectrolytes

Reye, John T.; J, Lu; Maxwell, Kendra E.; Banerjee, Sujit

doi:10.1016/j.biombioe.2011.10.011

Cited by 13 publications

(5 citation statements)

References 14 publications

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“…Some scientists have reported that the addition of nonionic surfactants could modify or block lignin surface properties and reduce the nonproductive adsorption of proteins. , Additionally, it was reported that the addition of a positively charged additive, polyvinylpyrrolidone (PVP), cationic polyelectrolytes, − improved the enzymatic hydrolysis efficiency of lignocellulose compared with that of a nonionic surfactant, which is consistent with our results in Figure . However, the mechanism should be further explored.…”

Section: Discussionsupporting

confidence: 90%

Alleviating Nonproductive Adsorption of Lignin on CBM through the Addition of Cationic Additives for Lignocellulosic Hydrolysis

Jiang

Wang

et al. 2022

ACS Appl. Bio Mater.

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The nonproductive adsorption of cellulase onto lignin significantly inhibited the enzymatic hydrolysis of lignocellulosic biomass. In this study, we constructed a rapid fluorescence detection (RFD) system, and using this system, we demonstrated that the addition of cationic additives DTAB or polyDADMAC greatly increased the partition coefficients of cellulose/lignin, reduced nonproductive adsorption, and enhanced the hydrolysis efficiency of lignocellulose compared to those of Tweens or PEGs. Moreover, the addition of polyDADMAC and DTAB increased the glucose yield released from the mixture of Avicel and AICS-lignin (MCL) by 16.9 and 20.6%, respectively, and reduced the inhibition rate of lignin by 16.9 and 20.7%, respectively. Interestingly, polyDADMAC or DTAB treatment performed more effectively for the enzymatic hydrolysis of pretreated lignocellulosic biomass, compared with MCL. We confirmed that the reduced hydrophobicity and increased zeta potential of lignin cocontribute to the dampening nonproductive adsorption of lignin. In particular, the zeta potential values of lignin and the partition coefficients of Avicel/lignin with the addition of additives showed a good correlation, suggesting that electrostatic force also plays a crucial role in the adsorbing of cellulase on lignin. This work will be conducive to decreasing the nonproductive binding of cellulase onto lignin and enhancing cellulose conversion.

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Section: Discussionsupporting

confidence: 90%

Alleviating Nonproductive Adsorption of Lignin on CBM through the Addition of Cationic Additives for Lignocellulosic Hydrolysis

Jiang

Wang

et al. 2022

ACS Appl. Bio Mater.

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“…The enzyme activity was previously determined as 137.04 FPU/mL according to the recommended NREL protocol . This value is similar to others reported by different authors in the literature. − The enzymatic hydrolysis was performed using a slightly modified method proposed by Rodrigues et al and Tan et al…”

Section: Methodssupporting

confidence: 70%

Scaled-Up Microwave-Assisted Pretreatment and Continuous Fermentation to Produce Yeast Lipids from Brewery Wastes

Longanesi

Bouxin

Fan

et al. 2020

Ind. Eng. Chem. Res.

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The cultivation of oleaginous yeast on second-generation feedstocks is an attractive alternative for edible lipid production. Despite many studies in this area addressing single aspects of this bioprocess, one of the major bottlenecks is the integration and optimization of the multiple unit operations while demonstrating this on a larger scale. In this investigation, a microwave-assisted hydrothermal process was developed to solubilize distillers’ dried grains with solubles (DDGS). The optimal MW process was run semicontinuously and produced a fermentable oligosaccharide-rich stream with negligible furan-based content. An overliming step and reverse-osmosis stage were demonstrated, increasing the solubilized carbohydrate content to over 60 g/L, suitably concentrated for further bioprocessing. The oleaginous yeast, Metschnikowia pulcherrima, was used to ferment the material and was demonstrated to metabolize up to 75% of the oligosaccharide pool and produced 41% lipid content at a concentration of 14 g/L in a 27 day semicontinuous process.

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“…Unfortunately, CE is relatively expensive and therefore inappropriate for industrial use at large scale. CTec 2, a new generation cellulase product, contains higher cellulase activity reported to vary from 119 to 132 FPU mL −1 ( Reye et al, 2011; McIntosh et al, 2012 ). To replace CE by using CTec 2 in the fermentation study, the appropriate CTec 2 dosage, in the form of a cocktail of “CTec 2 + BG”, was chosen which gave a comparative saccharification when compared with “CE + BG”.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre

Zhao

Moates

Elliston

et al. 2015

Bioresource Technology

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Enhancement of cellulase catalysis of wood pulp fiber by cationic polyelectrolytes

Cited by 13 publications

References 14 publications

Alleviating Nonproductive Adsorption of Lignin on CBM through the Addition of Cationic Additives for Lignocellulosic Hydrolysis

Alleviating Nonproductive Adsorption of Lignin on CBM through the Addition of Cationic Additives for Lignocellulosic Hydrolysis

Scaled-Up Microwave-Assisted Pretreatment and Continuous Fermentation to Produce Yeast Lipids from Brewery Wastes

Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre

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