Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation

Wang, Zhihao; Wang, Yun; Zhang, Dongxu; Li, Jianghua; Hua, Zhaozhe; Du, Guocheng; Chen, Jian

doi:10.1016/j.biortech.2009.09.025

Cited by 103 publications

(59 citation statements)

References 29 publications

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“…There are several studies on protein expression under methanol-induced AOX promoter, but the safe and robust constitutive GAP promoter has not been exploited fully at industrial level. The pGAPZαA vector was chosen because it allows the ORF fusion to α-factor mating signal sequence which has been used successfully in secretion of foreign proteins [10,11,37].…”

Section: Resultsmentioning

confidence: 99%

Bioprocess for efficient production of recombinant Pichia anomala phytase and its applicability in dephytinizing chick feed and whole wheat flat Indian breads

Joshi

Satyanarayana

2015

Journal of Industrial Microbiology and Biotechnology

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The phytase of the yeast Pichia anomala (PPHY) is a suitable biocatalyst as a food and feed additive because of its adequate thermostability, acid stability, protease insensitivity and broad substrate spectrum. The cell-bound nature and low phytase titres are the main bottlenecks for its utility in food and feed industries. In this investigation, we have overcome the problems by constitutive secretory expression of PPHY under glyceraldehyde phosphate dehydrogenase (GAP) promoter. A ~44-fold increase in rPPHY titre has been achieved after optimization of cultural variables by one-variable-at-a-time approach and two factorial statistical design. The use of GAP promoter makes the cultivation of the recombinant P. pastoris straight forward and eliminates the requirement of methanol for induction and hazards associated with its storage. Among metal-phytate complexes, Ca(2+) phytate is hydrolyzed more efficiently by rPPHY than Co(2+), Mn(2+), Mg(2+), Fe(3+) and Zn(2+) phytates. The enzyme is effective in dephytinizing whole wheat unleavened flat Indian breads (naan and tandoori) and different broiler feeds, thus mitigating anti-nutritional effects of phytates.

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Section: Resultsmentioning

confidence: 99%

Bioprocess for efficient production of recombinant Pichia anomala phytase and its applicability in dephytinizing chick feed and whole wheat flat Indian breads

Joshi

Satyanarayana

2015

Journal of Industrial Microbiology and Biotechnology

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“…The strategy of feeding mixed substrates has been applied in other fermentations. This was an effective method to decrease cell mortality, maintain the enzyme activity, and promote targeted metabolite production [9][10][11]. Similar studies with regard to glutamate fermentation are also important, but few have been reported.…”

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confidence: 85%

“…The concentrations of sorbitol and glycerol were analyzed by HPLC (Hitachi Chromaster Organizer, Hitachi, Ltd, Chiyoda-ku, Japan) equipped with an ion exclusion column (Aminex HPX-87H, 300 mm × 7.8 mm, Bio-Rad, Hercules, CA, USA) and a differential refractive index detector at 30°C. The mobile phase was 0.005 mol/L H 2 SO 4 at a flow rate of 0.6 mL/min [10]. Two electronic balances (JA1102, Haikang Co., Shanghai, China) were connected to the computer and used to monitor the feeding amount of glucose and sorbitol or glycerol solution.…”

Section: Analytical and Measurement Methodsmentioning

confidence: 99%

“…These auxiliary substrates are usually non-repressive carbon sources. They can protect cells against stress in their living environment, enhance cell viability, and reduce the metabolic burden [9][10][11][12]. It was reported that the production of alkaline polygalacturonate lyase and lipase increased by 1.85-fold and 8.7-fold, respectively, when the strategy of methanol/sorbitol co-feeding was adopted [10,13].…”

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confidence: 99%

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Improving the stability of glutamate fermentation by Corynebacterium glutamicum via supplementing sorbitol or glycerol

Cao

Shi

et al. 2015

Bioresour. Bioprocess.

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Background: Corynebacterium glutamicum is widely used in glutamate fermentation. The fermentation feature of the strain varies sometimes. These variations may lead to the reduction in the ability of the strain to resist environmental changes and to synthesize glutamate, resulting in abnormal glutamate fermentations. Results: In the abnormal glutamate fermentations, glutamate accumulation stopped after glucose feeding and the final glutamate concentration was at a lower level (50 to 60 g/L). The r NAD

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“…Most studies have reported the mixture of multicarbon substrates for P. pastoris growth and for foreign protein expression [Celik et al, 2009;Wang et al, 2010]. Few reports can be found on alternative convenient organic nitrogen sources for P. pastoris to express heterologous proteins.…”

Section: Discussionmentioning

confidence: 99%

High Expression of Human Cathepsin S by Recombinant <b><i>Pichia pastoris</i></b> with Cod Skin as an Organic Co-Nitrogen Source

Qin

et al. 2017

Microb Physiol

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Human cathepsin S production by recombinant Pichia pastoris using cod skin as the co-nitrogen source was investigated in this study. The addition of carbon sources of glycerol in the fed-batch phase and of methanol in the induction stage was also investigated. A new approach to the highly expression of human cathepsin S was developed using 90 g/L of cod skin (wet weight). After 24 h of the initial fermentation, 4% glycerol (v/v, glycerol/culture) was added once to enhance the cell density (OD₆₀₀) in the cultivation. Then, adding and maintaining methanol at 0.5% (v/v, methanol/cultivation) after about 48 h of fermentation achieved a high expression of human cathepsin S in a 5-L bioreactor. The results demonstrate that the maximum activity of human cathepsin S in the fermentation supernatant reached 7,152 U/L after 96 h of methanol induction. The methylotrophic yeast P. pastoris grown in the medium containing cod skin (90 g/L) as the co-nitrogen source provided a 21% higher cell density (OD₆₀₀) and 18.3% higher human cathepsin S yield than P. pastoris grown in BMGY medium. For the first time, human cathepsin S was successfully expressed by P. pastoris with cod skin as the co-nitrogen source. The glycerol fed-batch controlling strategy and method of maintaining methanol at a constant concentration of 0.5% (v/v, methanol/cultivation) in the induction stage was efficient for P. pastoris growth and the expression of human cathepsin S.

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Enhancement of cell viability and alkaline polygalacturonate lyase production by sorbitol co-feeding with methanol in Pichia pastoris fermentation

Cited by 103 publications

References 29 publications

Bioprocess for efficient production of recombinant Pichia anomala phytase and its applicability in dephytinizing chick feed and whole wheat flat Indian breads

Bioprocess for efficient production of recombinant Pichia anomala phytase and its applicability in dephytinizing chick feed and whole wheat flat Indian breads

Improving the stability of glutamate fermentation by Corynebacterium glutamicum via supplementing sorbitol or glycerol

High Expression of Human Cathepsin S by Recombinant <b><i>Pichia pastoris</i></b> with Cod Skin as an Organic Co-Nitrogen Source

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