Enhancement of catalytic performance of porcine pancreatic lipase immobilized on functional ionic liquid modified Fe3O4-Chitosan nanocomposites

Suo, Hongbo; Xu, Lili; Xu, Chao; Chen, Hongyue; Yu, Dinghua; Gao, Zhen; Huang, He; Hu, Yi

doi:10.1016/j.ijbiomac.2018.07.187

Cited by 68 publications

(36 citation statements)

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“…Different nanostructure materials have been used as support for the immobilization of enzymes. A variety of nanostructures such as nanoparticles, polymer nanofibers and nanocomposites, metal organic frameworks, nano-and mesoporous silica, carbon nanotubes, graphene, and graphene oxide have been used for enzyme immobilization [20][21][22][23][24]. The magnetic nanoparticles have been known as very useful material in different applications.…”

mentioning

confidence: 99%

Lipase immobilized on functionalized superparamagnetic few-layer graphene oxide as an efficient nanobiocatalyst for biodiesel production from Chlorella vulgaris bio-oil

Nematian

Shakeri

Salehi

et al. 2020

Biotechnol Biofuels

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Background: Microalgae, due to its well-recognized advantages have gained renewed interest as potentially good feedstock for biodiesel. Production of fatty acid methyl esters (FAMEs) as a type of biodiesel was carried out from Chlorella vulgaris bio-oil. Biodiesel was produced in the presence of nano-biocatalysts composed of immobilized lipase on functionalized superparamagnetic few-layer graphene oxide via a transesterification reaction. A hybrid of few-layer graphene oxide and Fe 3 O 4 (MGO) was prepared and characterized. The MGO was functionalized with 3-aminopropyl triethoxysilane (MGO-AP) as well as with a couple of AP and glutaraldehyde (MGO-AP-GA). The Rhizopus oryzae lipase (ROL) was immobilized on MGO and MGO-AP using electrostatic interactions as well as on MGO-AP-GA using covalent bonding. The supports, MGO, MGO-AP, and MGO-AP-GA, as well as nano-biocatalyst, ROL/MGO, ROL/ MGO-AP, and ROL/MGO-AP-GA, were characterized using FESEM, VSM, FTIR, and XRD. The few-layer graphene oxide was characterized using AFM and the surface charge of supports was evaluated with the zeta potential technique. The nano-biocatalysts assay was performed with an evaluation of kinetic parameters, loading capacity, relative activity, time-course thermal stability, and storage stability. Biodiesel production was carried out in the presence of nanobiocatalysts and their reusability was evaluated in 5 cycles of transesterification reaction. Results: The AFM analysis confirmed the few-layer structure of graphene oxide and VSM also confirmed that all supports were superparamagnetic. The maximum loading of ROL (70.2%) was related to MGO-AP-GA. The highest biodiesel conversion of 71.19% achieved in the presence of ROL/MGO-AP-GA. Furthermore, this nano-biocatalyst could maintain 58.77% of its catalytic performance after 5 cycles of the transesterification reaction and was the best catalyst in the case of reusability. Conclusions: In this study, the synthesized nano-biocatalyst based on bare and functionalized magnetic graphene oxide was applied and optimized in the process of converting microalgae bio-oil to biodiesel for the first time and compared with bare lipase immobilized on magnetic nanoparticles. Results showed that the loading capacity, kinetic parameters, thermal stability, and storage stability improved by the functionalization of MGO. The biocatalysts, which were prepared via covalent bonding immobilization of enzyme generally, showed better characteristics.

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mentioning

confidence: 99%

Lipase immobilized on functionalized superparamagnetic few-layer graphene oxide as an efficient nanobiocatalyst for biodiesel production from Chlorella vulgaris bio-oil

Nematian

Shakeri

Salehi

et al. 2020

Biotechnol Biofuels

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“…The new enzymatic derivatives obtained were named ionogels of CaLB. In our assay, the hydrophobic ionic liquid, [Omim][PF 6 ], was used because it creates a microenvironment that is suited to CaLB, thus increasing the enzyme activity over levels that can be obtained with hexane (used here as reference organic solvent) and other imidazolium-based ionic liquids with lower alkyl chain lengths that are commonly used as reaction media in transesterification reactions [ 7 , 14 , 26 , 27 , 28 ]. The entrapment method involving ionic liquids allows the enzyme to be immobilized by occluding CaLB within a suitable microenvironment that allows the enzyme to be retained while allowing the substrates and products of the reaction to cross the ionic liquid layer around the enzyme.…”

Section: Resultsmentioning

confidence: 99%

“…The activity of almost every PPL derivative was higher than that of the free enzyme when working above 50 °C. The thermal stability of the PPL derivatives at 50 °C ranged from 45% to 80% at 6 h [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

“…For example, in separation applications, they have been used as liquid–liquid biphasic systems [ 1 , 2 ] and as liquid phase in supported liquid membranes and polymer inclusion membranes [ 3 , 4 , 5 ]. In the field of biocatalysis, ionic liquids have been used as free solvent [ 6 , 7 , 8 ], adsorbed [ 9 , 10 , 11 , 12 ], covalently linked to particle enzymes [ 13 , 14 , 15 ], or as polymeric ionic liquids [ 16 ] in enzyme particles, in all cases to create an adequate microenvironment for the enzyme or to improve catalytic efficiency. The advantages of using ionic liquids instead of organic solvents as reaction media for biocatalysis include their ability to enhance enzyme activity as well as their selectivity and stability [ 6 , 7 , 8 , 9 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B

et al. 2020

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New Candida antarctica lipase B derivatives with higher activity than the free enzyme were obtained by occlusion in an organogel of an ionic liquid (ionogel) based on the ionic liquid [Omim][PF6] and polyvinyl chloride. The inclusion of glutaraldehyde as a crosslinker improved the properties of the ionogel, allowing the enzymatic derivative to reach 5-fold higher activity than the free enzyme and also allowing it to be reused at 70 °C. The new methodology allows enzymatic derivatives to be designed by changing the ionic liquid, thus providing a suitable microenvironment for the enzyme. The ionic liquid may act on substrates to increase their local concentration, while reducing water activity in the enzyme’s microenvironment. All this allows the activity and selectivity of the enzyme to be improved and greener processes to be developed. The chemical composition and morphology of the ionogel were also studied by scanning electron microscopy–energy dispersive X-ray spectroscopy, finding that porosity, which was related with the chemical composition, was a key factor for the enzyme activity.

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“…FTIR spectra of MWCNTs-COOH and mMWCNTs showed several peaks at 3,434 cm -1 , 1,626 cm -1 , 1,384 cm -1 , and 1,045 cm -1 ( Figure 1C). These peaks were ascribed to O-H bonds, 25 C=O groups, 26 and C-OH and C-O stretching vibrations, 27 respectively. The peak at 582 cm -1 of mMWCNTs ( Figure 1Cii) was assigned to metal oxygen.…”

Section: Characterization Of Mmwcntsmentioning

confidence: 99%

<p>Magnetic multiwalled carbon nanotubes with controlled release of epirubicin: an intravesical instillation system for bladder cancer</p>

Suo¹,

Wang²,

Jin³

et al. 2019

IJN

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Background: Traditional intravesical instillation treatment in bladder cancer has limited efficacy, which results in a high frequency of recurrence. Purpose: The aim of this study was to report on an epirubicin (EPI)-loaded magnetic multiwalled carbon nanotube (mMWCNTs-EPI) system for intravesical instillation in place of the current formulation. Methods: The mMWCNTs-EPI system was formulated with carboxylated MWCNTs, Fe 3 O 4 magnetic nanoparticles, and EPI. Features and antitumor activity of the system were investigated. Results: Under the effect of external magnets, the mMWCNTs-EPI system showed sustained release and prolonged retention behavior and better antitumor activity than free EPI. The mMWCNTs-EPI system had higher efficiency in enhancing cytotoxicity and inhibiting proliferation in vitro and in vivo than free EPI. Our studies also revealed the atoxic nature of mMWCNTs. Conclusion: These findings suggested that mMWCNTs are effective intravesical instillation agents with great potential for clinical application.

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Enhancement of catalytic performance of porcine pancreatic lipase immobilized on functional ionic liquid modified Fe3O4-Chitosan nanocomposites

Cited by 68 publications

References 50 publications

Lipase immobilized on functionalized superparamagnetic few-layer graphene oxide as an efficient nanobiocatalyst for biodiesel production from Chlorella vulgaris bio-oil

Lipase immobilized on functionalized superparamagnetic few-layer graphene oxide as an efficient nanobiocatalyst for biodiesel production from Chlorella vulgaris bio-oil

Immobilization in Ionogel: A New Way to Improve the Activity and Stability of Candida antarctica Lipase B

<p>Magnetic multiwalled carbon nanotubes with controlled release of epirubicin: an intravesical instillation system for bladder cancer</p>

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