Enhancement of carcinostatic activity of ω-hydroxy fatty acids by their esterification through increased uptake into tumor cells

Kusumoto, Kumiko; Kageyama, Katsuhiro; Tanaka, Hiroshi; Kogawa, Hiroshi; Miwa, Nobuhiko

doi:10.3892/or.11.4.857

Cited by 7 publications

(9 citation statements)

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“…It can be avoided by (i) blocking the carboxylic group as iron carboxylate before decomposition and (ii) scavenging the ω-hydroxyacid freed by the decomposition of the carboxylate precursor by using an oligoethylene glycol as solvent. Reactants and solvent are cheap and have low toxicity [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

One-step synthesis and functionalization of hydroxyl-decorated magnetite nanoparticles

Mondini

Cenedese

Marinoni

et al. 2008

Journal of Colloid and Interface Science

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Section: Introductionmentioning

confidence: 99%

One-step synthesis and functionalization of hydroxyl-decorated magnetite nanoparticles

Mondini

Cenedese

Marinoni

et al. 2008

Journal of Colloid and Interface Science

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“…The viability of cells was measured using two different methods: i) the redox indicator dye WST-1, which becomes more chromic according to mitochondrial dehydrogenase activity, and ii) the trypan blue dye-exclusion assay. For WST-1 (Cell Counting Kit, Dojin Chemicals, Kumamoto, Japan) (18,19), the cultured cell suspension was transferred to a sampling tube and centrifuged. The resultant supernatant was completely removed from the tube and 110 μl of WST-1 (8%) per well was added to the cell precipitate, suspended and transferred to each well of a 96-well microplate.…”

Section: Methodsmentioning

confidence: 99%

“…Synergistic enhancement of the effects of Tonalide and Pearlide was observed by applying them in combination with hyperthermia, which is known to inhibit the growth (12)(13)(14) and DNA synthesis (15)(16)(17) of tumor cells. Carcinostatic effect was evaluated as a decrease in mitochondrial dehydrogenase activity reflecting the survival rate of cells as measured by the WST-1 assay (18,19), and also as a decrease in viable cells according to the degree of staining determined by the trypan blue dyeexclusion assay (20,21). Lifespan-prolonging effects on EAT cell-transplanted mice were investigated to evaluate whether the substances could be safely administered to cancer patients as anticancer drugs (4).…”

Section: Introductionmentioning

confidence: 99%

The antitumor activities of the structurally-similar two-species aromatics Tonalide and Pearlide and the enhancement of their effects by hyperthermia

Asada

Kageyama²,

Tanaka³

et al. 2008

Mol Med Rep

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Abstract. Antitumor activities have been reported for the aromatics Tonalide (6-acetyl-1,1,2,4,4,7-hexamethyl-tetrahydronaphthalene, AHTN) and Pearlide (1,3,4,6,7,6,6,7,8, HHCB), which are contained in detergents. In this study, their carcinostatic activities in Ehrlich ascites tumor (EAT) cells were evaluated by mitochondrial dehydroganase-based WST-1 assay and dye-exclusion assay. The viability of EAT cells treated at 37 or 42˚C for 30 min and sequentially cultured at 37˚C was assayed at graded times. Immediately after treatment at 37˚C, neither Tonalide nor Pearlide had an effect on EAT cells, even at a concentration as high as 200 μM. However, cell viability was reduced to 40% versus the control after 20 h of culture with Tonalide at 50 μM, and to below 20% at 25 μM after 72 h. In contrast, Pearlide was nearly inactive, even at a dose of 100 μM after 20 h of culture, and only reduced cell viability to 41.2% after 72 h. After treatment at 42˚C without culture, neither of the aromatics was effective, even at a dose of 200 μM. The viability of cells cultured with Tonalide for 20 h after treatment at 42˚C was reduced to nearly half of that at 37˚C, and to 10% of the control after culture for 72 h. These values for the reduction of cell viability were also acheived by the Trypan blue dye-exclusion assay. The lifespan-prolonging effects of Tonalide on mice implanted with EAT cells were also examined. The lifespan of mice administrated 1.8 mg/day of Tonalide was prolonged by up to 28 days, while mice that did not receive Tonalide died within a mean of 15 days. Thus, Tonalide exhibited marked carcinostatic effects in vitro and in vivo and may prove to be a potent multipurpose antitumor agent in combination with hyperthermia.

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“…Carcinostatic effect was evaluated with a decrease in mitochondrial dehydrogenase activity reflecting survival rate of cells as measured by WST-8 assay (13,14) and with a decrease in viable cells observed morphologically by Crystal Violet stain assay (15). Morphological cellular degeneration was observed by scanning electron microscopy (SEM) (16,17).…”

Section: Introductionmentioning

confidence: 99%

Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death

Kageyama¹

2010

Oncol Rep

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Abstract. In order to erase reactive oxygen species (ROS) related with the proliferation of tumor cells by reducing activity of hydrogen, we developed functional water containing nano-bubbles (diameters: <900 nm for 71%/ population) hydrogen of 1.1-1.5 ppm (the theoretical maximum: 1.6 ppm) with a reducing ability (an oxidationreduction potential -650 mV, normal water: +100-200 mV) using a microporous-filter hydrogen-jetting device. We showed that hydrogen water erased ROS indispensable for tumor cell growth by ESR/spin trap, the redox indicator CDCFH-DA assay, and was cytotoxic to Ehrlich ascites tumor cells as assessed by WST-8 assay, crystal violet dye stain and scanning electron microscopy, after 24-h or 48-h incubation sequent to warming at 37˚C or 42˚C. Hydrogen water supplemented with platinum colloid (0.3 ppm Pt in 4% polyvinylpyrrolidone) had more antitumor activity than hydrogen water alone, mineral water alone (15.6%), hydrogen water plus mineral water, or platinum colloid alone as observed by decreased cell numbers, cell shrinkage and pycnosis (nuclear condensation)/karyorrhexis (nuclear fragmentation) indicative of apoptosis, together with cell deformation and disappearance of microvilli on the membrane surface. These antitumor effects were promoted by combination with hyperthermia at 42˚C. Thus, the nano-bubble hydrogen water with platinum colloid is potent as an antitumor agent. IntroductionTumor cells produce reactive oxygen species (ROS) more abundantly than normal cells (1). Behavior of ROS is suggested to be related to the promotion of cell proliferation, DNA synthesis (2), survival, cellular migration (3), invasion (4), tumor metastasis (5), and angiogenesis (6). Accordingly, anti-oxidants counteract with ROS, and can inhibit tumor cell proliferation (7). The water containing abundant hydrogen, an antioxidant, can erase ROS and is expected to have antitumor activity (8).An apparatus which can produce the functional water containing nano-bubble hydrogen has been developed (8). The water contains hydrogen nano-bubbles <900-nm diameter, for 71% of total bubbles, and hydrogen of 1.1-1.5 ppm, and has an oxidation-reduction potential (ORP) of -650 mV versus +100-200 mV in normal water. The nano-babble hydrogen-enriched water reduces hydroxyl radicals in normal water to a quarter of the initial amount (8). However, the cell culture medium which was made based on this hydrogen water lost hydrogen during the subsequent adjustment, and heightened the ORP remarkably, and there was little antitumor activity of this hydrogen culture medium alone. Therefore, the effect has been acquired by adding the platinum particle (9).We improved the device and made a compact type of hydrogen water manufacturing device with a microporousfilter hydrogen-jetting nozzle that was able to jet hydrogen gas into the culture medium directly. As a result, the hydrogen dissolved culture medium showed the oxidation-reduction potential of -650 mV even before the experiment. This value was maintained for a period as long as three m...

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Enhancement of carcinostatic activity of ω-hydroxy fatty acids by their esterification through increased uptake into tumor cells

Cited by 7 publications

References 0 publications

One-step synthesis and functionalization of hydroxyl-decorated magnetite nanoparticles

One-step synthesis and functionalization of hydroxyl-decorated magnetite nanoparticles

The antitumor activities of the structurally-similar two-species aromatics Tonalide and Pearlide and the enhancement of their effects by hyperthermia

Antitumor effects of nano-bubble hydrogen-dissolved water are enhanced by coexistent platinum colloid and the combined hyperthermia with apoptosis-like cell death

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