Enhancement of Bone-Marrow-Derived Mesenchymal Stem Cell Angiogenic Capacity by NPWT for a Combinatorial Therapy to Promote Wound Healing with Large Defect

Abstract

Poor viability of engrafted bone marrow mesenchymal stem cells (BMSCs) often hinders their application for wound healing, and the strategy of how to take full advantage of their angiogenic capacity within wounds still remains unclear. Negative pressure wound therapy (NPWT) has been demonstrated to be effective for enhancing wound healing, especially for the promotion of angiogenesis within wounds. Here we utilized combinatory strategy using the transplantation of BMSCs and NPWT to investigate whether this combinatory therapy could accelerate angiogenesis in wounds. In vitro, after 9-day culture, BMSCs proliferation significantly increased in NPWT group. Furthermore, NPWT induced their differentiation into the angiogenic related cells, which are indispensable for wound angiogenesis. In vivo, rat full-thickness cutaneous wounds treated with BMSCs combined with NPWT exhibited better viability of the cells and enhanced angiogenesis and maturation of functional blood vessels than did local BMSC injection or NPWT alone. Expression of angiogenesis markers (NG2, VEGF, CD31, and α-SMA) was upregulated in wounds treated with combined BMSCs with NPWT. Our data suggest that NPWT may act as an inductive role to enhance BMSCs angiogenic capacity and this combinatorial therapy may serve as a simple but efficient clinical solution for complex wounds with large defects.

Enhancing bone regeneration by combining mesenchymal stem cell sheets with β‐TCP/COL‐I scaffolds

“…Tissue regeneration using stem cells is one of the most promising techniques used to reestablish the integrity of injured tissues . The regeneration of bone defects resulting from trauma, infection, tumors, or congenital malformation still remains a clinical challenge . Autogenous bone grafting is still considered the golden standard for bone regeneration and remains the most commonly used surgical technique at present .…”

Intrinsic Angiogenic Potential and Migration Capacity of Human Mesenchymal Stromal Cells Derived from Menstrual Blood and Bone Marrow

“…In order to avoid residual contamination of CD31+ cells due to the heterogeneity of MSC cultures in initial passages, we used cells from passage 5 in this experiment. Like other mesenchymal stromal cells, mbMSC and bmMSC ordinarily did not express CD31 [ 32 , 35 , 37 , 62 , 63 , 64 ]. However, in this work both MSC appear to be going through endothelial differentiation, since there were positive cells for CD31.…”

Influence of negative pressure wound therapy on peri‐prosthetic tissue vascularization and inflammation around porous titanium percutaneous devices

“…One limitation of this study was the small sample size. The sample size used in the present study ( n = 6) was based on the epithelial downgrowth data around percutaneous devices from Mitchell et al and blood vessel density data from a NPWT study in open wounds, since blood vessel densities around percutaneous devices have not been previously quantified. Lastly, to the authors' knowledge, there are no previously published NPWT based animal studies which quantify CD68 positive cell densities; therefore, a similar effect size as blood vessel densities was assumed.…”