Enhancement of biomass, lipids, and polyunsaturated fatty acid (PUFA) production in Nannochloropsis oceanica with a combination of single wavelength light emitting diodes (LEDs) and low temperature in a three-phase culture system

Sirisuk, Phunlap; Sunwoo, InYung; Kim, Sohee; Awah, Clovis; Hun, Chae; Kim, Jongmyung; Jeong, Gwi‐Taek; Kim, Sung-Koo

doi:10.1016/j.biortech.2018.09.025

Cited by 38 publications

(11 citation statements)

References 32 publications

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“…These features enable a high rate of carbon fixation with the potential for highly efficient biodiesel production. Furthermore, microalgae can be easily grown on non-arable land, which avoids competition with food production [6][7][8].…”

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confidence: 99%

Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether

Wang

Oshita

Takaoka

2021

Biotechnol Biofuels

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Background Recent studies of lipid extraction from microalgae have focused primarily on dewatered or dried samples, and the processes are simple with high lipid yield. Yet, the dewatering with drying step is energy intensive, which makes the energy input during the lipid production more than energy output from obtained lipid. Thus, exploring an extraction technique for just a thickened sample without the dewatering, drying and auxiliary operation (such as cell disruption) is very significant. Whereas lipid extraction from the thickened microalgae is complicated by the high water content involved, and traditional solvent, hence, cannot work well. Dimethyl ether (DME), a green solvent, featuring a high affinity for both water and organic compounds with an ability to penetrate the cell walls has the potential to achieve this goal. Results This study investigated an energy-saving method for lipid extraction using DME as the solvent with an entrainer solution (ethanol and acetone) for flocculation-thickened microalgae. Extraction efficiency was evaluated in terms of extraction time, DME dosage, entrainer dosage, and ethanol:acetone ratio. Optimal extraction occurred after 30 min using 4.2 mL DME per 1 mL microalgae, with an entrainer dosage of 8% at 1:2 ethanol:acetone. Raw lipid yields and its lipid component (represented by fatty acid methyl ester) contents were compared against those of common extraction methods (Bligh and Dryer, and Soxhlet). Thermal gravimetry/differential thermal analysis, Fourier-transform infrared spectroscopy, and C/H/N elemental analyses were used to examine differences in lipids extracted using each of the evaluated methods. Considering influence of trace metals on biodiesel utilization, inductively coupled plasma mass spectrometry and inductively coupled plasma atomic emission spectroscopy analyses were used to quantify trace metals in the extracted raw lipids, which revealed relatively high concentrations of Mg, Na, K, and Fe. Conclusions Our DME-based method recovered 26.4% of total raw lipids and 54.4% of total fatty acid methyl esters at first extraction with remnants being recovered by a 2nd extraction. In additional, the DME-based approach was more economical than other methods, because it enabled simultaneous dewatering with lipid extraction and no cell disruption was required. The trace metals of raw lipids indicated a purification demand in subsequent refining process.

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confidence: 99%

Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether

Wang

Oshita

Takaoka

2021

Biotechnol Biofuels

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“…This level of EPA was similar to that reported in [30] (23.4%). Other studies [33,34] indicated a lower range of EPA occurring in N. oceanica, from 1.16 to 10.12% of the TFA, depending on the culture medium. Consequently, the total PUFA and LC-PUFA levels of N. oceanica were high at 40.4%, and 30.0% of TFA in this study, respectively.…”

Section: Discussionmentioning

confidence: 90%

Fatty Acid Profiles of Selected Microalgae Used as Live Feeds for Shrimp Postlarvae in Vietnam

Duc

Lee-Chang

Jameson

et al. 2021

Aquaculture Journal

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The importance of microalgal lipids for the survival and growth of shrimp postlarvae has been recognized in a range of studies. Microalgae with fast growth rates and high levels of polyunsaturated fatty acids (PUFA) are considered vital to maximise production and minimise cost in shrimp larviculture. The lipid content and fatty acid composition of microalgae used in shrimp production varies substantially between the algal classes and species being used in Vietnam. This study aims to characterise microalgal lipid and fatty acid (FA) profiles and evaluate the most promising species under growth conditions that are most suitable for shrimp aquaculture. Here, we report that the highest lipid contents were obtained in the Haptophyta microalgae, Tisochrysis lutea and Isochrysis galbana, at 90.3 and 61.1 mg/g, respectively. In contrast, two of the most popular diatom species being used for shrimp larval cultivation in Vietnam, Thalassiosira pseudonana and T. weissflogii, displayed the lowest lipid contents at 16.1 mg/g. Other microalgal species examined showed lipid contents ranging from 28.6 to 55 mg/g. Eicosapentaenoic acid (EPA, 20:5ω3) ranged from 0.6 to 29.9% across the species, with docosahexaenoic acid (DHA, 22:6ω3) present at 0.01 to 11.1%; the two omega (ω)–3 long-chain (LC, ≥C20) LC-PUFA varied between the microalgae groups. Polar lipids were the main lipid class, ranging from 87.2 to 97.3% of total lipids, and triacylglycerol was detected in the range of 0.01 to 2.5%. Saturated fatty acids (SFA) and monounsaturated fatty acids (MUFA) increased and PUFA decreased with increasing growth temperatures. This study demonstrated the differences in the lipid contents and FA profiles across 10 microalgal species and the effect of the higher temperature growing conditions encountered in Vietnam.

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“…However, excessive aeration rates can cause shear stress to the cells, causing cell damage [23]. Sirisuk et al [15] reported that increasing the aeration rate from 0.25 vvm to 0.50 vvm increases the cellular biomass from 0.33 g dcw/l to 0.38 g dcw/l in Nannochloropsis oceanica cultures. To obtain large microalgal biomasses, a high aeration rate and a high level of CO 2 are required; however, the aeration rate must be limited due to the eventual cell damage [24].…”

Section: Effect Of Aeration Rate Under Green Led Wavelengthmentioning

confidence: 99%

Improvement of Unsaturated Fatty Acid Production from Porphyridium cruentum Using a Two-Phase Culture System in a Photobioreactor with Light-Emitting Diodes (LEDs)

Kim

Lee

et al. 2021

J. Microbiol. Biotechnol.

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In this study, the culture conditions for Porphyridium cruentum were optimized to obtain the maximum biomass and lipid productions. The eicosapentaenoic acid content was increased by pH optimization. P. cruentum was cultured with modified F/2 medium in 14-L photobioreactors using a two-phase culture system, in which the green (520 nm) and red (625 nm) light-emitting diodes (LEDs) were used during the first and second phases for biomass production and lipid production, respectively. Various parameters, including aeration rate, light intensity, photoperiod, and pH were optimized. The maximum biomass concentration of 0.91 g dcw/l was obtained with an aeration rate of 0.75 vvm, a light intensity of 300 μmol m -2 s -1 , and a photoperiod of 24:0 h. The maximum lipid production of 51.8% (w/w) was obtained with a light intensity of 400 μmol m -2 s -1 and a photoperiod of 18:6 h. Additionally, the eicosapentaenoic acid and unsaturated fatty acid contents reached 30.6% to 56.2% at pH 6.0.

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Enhancement of biomass, lipids, and polyunsaturated fatty acid (PUFA) production in Nannochloropsis oceanica with a combination of single wavelength light emitting diodes (LEDs) and low temperature in a three-phase culture system

Cited by 38 publications

References 32 publications

Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether

Effective lipid extraction from undewatered microalgae liquid using subcritical dimethyl ether

Fatty Acid Profiles of Selected Microalgae Used as Live Feeds for Shrimp Postlarvae in Vietnam

Improvement of Unsaturated Fatty Acid Production from Porphyridium cruentum Using a Two-Phase Culture System in a Photobioreactor with Light-Emitting Diodes (LEDs)

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