Enhancement of beta-catenin in cardiomyocytes suppresses survival protein expression but promotes apoptosis and fibrosis

Lin, James Chi-Jen; Kuo, Wei Wen; Baskaran, Rathinasamy; Chen, Ming Cheng; Ho, Tsung Jung; Chen, Ray Jade; Chen, Ya-Fang; Padma, Viswanadha Vijaya; Lay, Ing Shiow; Huang, Chih‐Yang

doi:10.5603/cj.a2016.0087

Cited by 21 publications

(11 citation statements)

References 41 publications

(58 reference statements)

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“…β-catenin has been used as a marker of EMT in a number of studies of embryonic development, cancer, and fibrosis ( 30 – 33 ). According to previous studies, β-catenin is associated with EMT during renal fibrosis ( 34 ) and fibrosis in other organs ( 35 , 36 ). In addition, β-catenin participates in the development of PF by transforming A549 cells into fibroblasts ( 23 , 37 ).…”

Section: Discussionmentioning

confidence: 86%

Hypoxia-inducible factor-1α regulates epithelial-to-mesenchymal transition in paraquat-induced pulmonary fibrosis by activating lysyl oxidase

et al. 2017

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Pulmonary fibrosis (PF) is one of the most prevalent causes of death following paraquat (PQ) poisoning. As demonstrated in previous studies by the present authors, epithelial-to-mesenchymal transition (EMT) is associated with PQ-induced PF. In addition, hypoxia-inducible factor-1α (HIF-1α) and lysyl oxidase (LOX) promote EMT following PQ poisoning. However, the association between HIF-1α- and LOX-mediated regulation of EMT remains unclear. The present study investigated the association between HIF-1α and LOX with regard to PQ-induced EMT. A549 and RLE-6TN cells were treated with PQ, and HIF-1α and LOX expression was silenced with short interfering RNAs. Changes in the expression of HIF-1α, LOX, β-catenin and EMT-related makers were detected using real-time quantitative polymerase chain reaction, immunofluorescence, and western blotting. HIF-1α and LOX were associated with PQ-induced EMT, and their expression levels were significantly increased (P<0.05). LOX expression was significantly decreased following PQ poisoning when HIF-1α expression was inhibited (P<0.05). However, the level of HIF-1α did not change significantly when LOX was silenced. The expression level of β-catenin and the degree of EMT were significantly decreased following HIF-1α and LOX silencing in both cell lines (P<0.05). The association between HIF-1α and LOX in regulating EMT during PQ-induced PF may be unidirectional. HIF-1α may regulate PQ-induced EMT through the LOX/β-catenin pathway.

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Section: Discussionmentioning

confidence: 86%

Hypoxia-inducible factor-1α regulates epithelial-to-mesenchymal transition in paraquat-induced pulmonary fibrosis by activating lysyl oxidase

et al. 2017

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“…Our earlier studies have shown that abnormal activation of IGF-IIR signaling pathway during cardiac stresses such as elevated Ang II levels [3], or high glucose [5], or drug-induced (doxorubicin) cardiotoxicity [12,13] could lead to increased hypertrophy, fibrosis, and cardiac apoptosis paving the way for heart failure. Besides, we showed that enhancement of β-catenin in cardiomyocytes promotes apoptosis and fibrosis during cardiac stresses such as myocardial infarction (MI) or diabetes [14]. This study showed that Ang-II treatment could upregulate β-catenin expression in parallel with IGF-IIR expression in a dose-dependent assessment.…”

Section: Discussionmentioning

confidence: 98%

β-catenin/LEF1/IGF-IIR Signaling Axis Galvanizes the Angiotensin-II- induced Cardiac Hypertrophy

Lai

Pandey

Day

et al. 2019

IJMS

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Cardiovascular diseases have a high prevalence worldwide and constitute the leading causes of mortality. Recently, malfunctioning of β-catenin signaling has been addressed in hypertensive heart condition. Ang-II is an important mediator of cardiovascular remodeling processes which not only regulates blood pressure but also leads to pathological cardiac changes. However, the contribution of Ang-II/β-catenin axis in hypertrophied hearts is ill-defined. Employing in vitro H9c2 cells and in vivo spontaneously hypertensive rats (SHR) cardiac tissue samples, western blot analysis, luciferase assays, nuclear-cytosolic protein extracts, and immunoprecipitation assays, we found that under hypertensive condition β-catenin gets abnormally induced that co-activated LEF1 and lead to cardiac hypertrophy changes by up-regulating the IGF-IIR signaling pathway. We identified putative LEF1 consensus binding site on IGF-IIR promoter that could be regulated by β-catenin/LEF1 which in turn modulate the expression of cardiac hypertrophy agents. This study suggested that suppression of β-catenin expression under hypertensive condition could be exploited as a clinical strategy for cardiac pathological remodeling processes.

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“…In skeletal muscle tissue, nuclear β‐catenin has been shown to be upregulated in response to mechanical overload, which was mediated via the canonical Wnt/β‐catenin pathway 44 . Overexpression of cardiac β‐catenin was associated with cardiac hypertrophy, with increased fibrosis and with apoptosis in vitro 45,46 47 .…”

Section: Discussionmentioning

confidence: 99%

Reaming of femoral fractures with different reaming irrigator aspirator systems shows distinct effects on cardiac function after experimental polytrauma

Lackner

Weber

Miclau

et al. 2020

Journal Orthopaedic Research

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Cardiac injuries are recorded after multiple trauma and are associated with a poor patient outcome. Reaming prior to locked intramedullary nailing is a frequently used technique to stabilize femoral diaphysis fractures. However, in polytraumatized patients, complications such as fat emboli and acute respiratory distress syndrome have been associated with reaming. The reaming irrigator aspirator (RIA) system provides concomitant irrigation and suction of the intramedullary contents, and should, therefore, reduce reaming‐associated complications. The aim of the study was to investigate cardiac function after multiple trauma with regard to two different RIA devices (RIAI vs RIAII). 15 male pigs were included in the study. Pigs received either sham treatment or multiple trauma (chest trauma, femur fracture, liver laceration, and hemorrhagic shock), followed by intramedullary nailing after reaming with either the RIAI or RIAII system (RIAII: reduced diameter of the reamer, improved control of irrigation and suction). Cardiac function was assessed by transesophageal echocardiography and systemic inflammation as well as local cardiac damage examined. Pigs of both treatment groups showed impaired cardiac function, valvular insufficiency, and cardiac damage. Systemic inflammation and local cardiac alterations were observed which might contribute to early myocardial damage in vivo. Multiple trauma including long‐bone fracture and subsequent intramedullary reaming induces cardiac dysfunction and valvular insufficiency, which might be linked to both mechanical cardiac injury and increased systemic inflammation. 6 hours after trauma there are less differences between RIAI and RIAII treatment with regard to post‐traumatic cardiac consequences in multiple injured pigs, indicating no beneficial effect of RIAII over RIAI.

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Enhancement of beta-catenin in cardiomyocytes suppresses survival protein expression but promotes apoptosis and fibrosis

Abstract: (Cardiol J 2017; 24, 2: 195-205)

Cited by 21 publications

References 41 publications

Hypoxia-inducible factor-1α regulates epithelial-to-mesenchymal transition in paraquat-induced pulmonary fibrosis by activating lysyl oxidase

Hypoxia-inducible factor-1α regulates epithelial-to-mesenchymal transition in paraquat-induced pulmonary fibrosis by activating lysyl oxidase

β-catenin/LEF1/IGF-IIR Signaling Axis Galvanizes the Angiotensin-II- induced Cardiac Hypertrophy

Reaming of femoral fractures with different reaming irrigator aspirator systems shows distinct effects on cardiac function after experimental polytrauma

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