Enhancement of bacterial growth with the help of immiscible oxygenated oils

Abstract

Bacterial growth in an aqueous medium in the vicinity of the interface with an immiscible oxygenated fluid is a subject of this study. We tested six oxygenated liquids, including hexadecane, silicone oil, FC-40, FC-70, HFE-7200 and HFE-7500.

New tools for recombinant protein production in Escherichia coli: A 5‐year update

“…For example, controlled carbon source supply in shake flasks has been achieved by controlled release of glucose by its diffusion from silicone beads or in situ degradation of polysaccharides . Oxygen availability can be modulated by proper flask design or by adding immiscible oxygenated oils . Lastly, devices that monitor these parameters and others have been adapted for cultures in microplates and shake flasks so that the best conditions for recombinant protein production can be found .…”

Gravity-driven microfluidic assay for digital enumeration of bacteria and for antibiotic susceptibility testing

“…In our case, roughly 1/3 of the volume of the chamber is filled by 2 nl droplets. Using the solubility coefficients of oxygen in complex medium 28 and in fluorocarbon oil, 29 we can calculate the amount of oxygen per droplet in the chamber. To estimate the oxygen consumption by bacteria inside a droplet, we assume that initially there is one bacterium inside the droplet which consumes oxygen with a rate 30 determined by the exponential growth of bacteria.…”

Continuous Recirculation of Microdroplets in a Closed Loop Tailored for Screening of Bacteria Cultures

“…This effect was beneficial because the continuous phase was used as a carrier of oxygen or other substances needed to run the reactions inside droplets [ 43 ]. It was shown that the continuous phase of oxygenated oil amplified bacterial growth in the water phase [ 44 ]. In the presented loop-circulation, droplets had contact with fresh oil, which was continuously changing between containers and tubing.…”