Enhancement of arsenic uptake and accumulation in green microalga Chlamydomonas reinhardtii through heterologous expression of the phosphate transporter DsPht1

Overexpression of a MYB1 Transcription Factor Enhances Triacylglycerol and Starch Accumulation and Biomass Production in the Green Microalga Chlamydomonas reinhardtii

“…The sequences of the construct were confirmed by sequencing (Macrogen), and the overexpression construct of MYB1 is shown schematically in Figure S1. Transformation was performed by electroporation as previously described. , Briefly, algal cells at exponential phase (1 × 10 8 cells/mL) were mixed with 1 μg of linearized plasmid DNA. Electroporation was performed by Bio-Rad Gene Pulser Xcell (Voltage 500 V, Capacity 50 μF and Resistance 800 Ω).…”

“…The chlorophyll fluorescence measurements were performed as we previously described. , Briefly, the MYB1 -OE transformants and PL cells were incubated in dark for 10 min. Thereafter, the chlorophyll fluorescence (the maximum photochemical efficiency of PSII, F v /F m ), and nonphotochemical quenching (NPQ) were measured with a pulse amplitude-modulated (PAM) fluorometry (Water-PAM WALZ, Germany).…”

“…Transformation was performed by electroporation as previously described. 26,27 Briefly, algal cells at exponential phase (1 × 10 8 cells/ mL) were mixed with 1 μg of linearized plasmid DNA. Electroporation was performed by Bio-Rad Gene Pulser Xcell (Voltage 500 V, Capacity 50 μF and Resistance 800 Ω).…”

Novel Phosphate Transporter-B PvPTB1;1/1;2 Contribute to Efficient Phosphate Uptake and Arsenic Accumulation in As-Hyperaccumulator Pteris vittata

“…20 After Shin et al identified the first P transporter with AsV transport capacity in Arabidopsis thaliana, several Pht1 proteins have been identified to participate in both AsV and/or P uptake and transport in different plants. They include A. thaliana, 21−25 rice, 26−29 P. vittata, 5−10 wheat, 30 Chlamydomonas reinhardtii, 31 and Panax notoginseng. 32,33 In plants, the Pht1 family localizes at the plasma membrane, mediating P and AsV uptake from the rhizosphere into plant roots in an H + -coupled manner.…”

“…thaliana, − rice, − P. vittata, − wheat, Chlamydomonas reinhardtii, and Panax notoginseng. , In plants, the Pht1 family localizes at the plasma membrane, mediating P and AsV uptake from the rhizosphere into plant roots in an H + -coupled manner . Since Pht1’s affinity for P is often greater than AsV, most plants have limited capacity for AsV uptake.…”