Enhancement of antiviral capacity of transgenic silkworms against cytoplasmic polyhedrosis virus via knockdown of multiple viral genes

Jiang, Liang; Peng, Zhengwen; Guo, Huizhen; Sun, Jingchen; Sun, Qiang; Xia, Fei; Huang, Ching-Hua; Xu, Guowen; Xia, Qingyou

doi:10.1016/j.dci.2017.07.020

Cited by 18 publications

(6 citation statements)

References 10 publications

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“…Transgenic silkworms with high antiviral capacity have been constructed ( 102 , 103 , 108 , 109 ). Nevertheless, their commercial application still faces great challenges.…”

Section: Major Issues In Silkworm Antiviral Studiesmentioning

confidence: 99%

Advances in the Arms Race Between Silkworm and Baculovirus

2021

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Insects are the largest group of animals. Nearly all organisms, including insects, have viral pathogens. An important domesticated economic insect is the silkworm moth Bombyx mori. B. mori nucleopolyhedrovirus (BmNPV) is a typical baculovirus and a primary silkworm pathogen. It causes major economic losses in sericulture. Baculoviruses are used in biological pest control and as a bioreactor. Silkworm and baculovirus comprise a well-established model of insect–virus interactions. Several recent studies have focused on this model and provided novel insights into viral infections and host defense. Here, we focus on baculovirus invasion, silkworm immune response, baculovirus evasion of host immunity, and enhancement of antiviral efficacy. We also discuss major issues remaining and future directions of research on silkworm antiviral immunity. Elucidation of the interaction between silkworm and baculovirus furnishes a theoretical basis for targeted pest control, enhanced pathogen resistance in economically important insects, and bioreactor improvement.

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“…Transgenic silkworms with high antiviral capacity have been constructed ( 102 , 103 , 108 , 109 ). Nevertheless, their commercial application still faces great challenges.…”

Section: Major Issues In Silkworm Antiviral Studiesmentioning

confidence: 99%

Advances in the Arms Race Between Silkworm and Baculovirus

2021

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“…In fact, this approach has been demonstrated to be effective in a commercially valuable silkworm strain (Isobe et al, 2004; Kanginakudru et al, 2007; Subbaiah et al, 2013). In addition, a similar strategy has been successfully tested to obtain protection of the silkworm to the B. mori cytoplasmic polyhedrosis virus (BmCPV) (Jiang et al, 2017). At last, injection of virus-specific dsRNA has been demonstrated to protect the mealworm beetle, Tenebrio molitor , against viral infection as well (Valdes et al, 2003).…”

Section: Insect Viral Infections and Rnai-based Antiviral Immunitymentioning

confidence: 99%

RNA Interference in Insects: Protecting Beneficials and Controlling Pests

et al. 2019

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Insects constitute the largest and most diverse group of animals on Earth with an equally diverse virome. The main antiviral immune system of these animals is the post-transcriptional gene-silencing mechanism known as RNA(i) interference. Furthermore, this process can be artificially triggered via delivery of gene-specific double-stranded RNA molecules, leading to specific endogenous gene silencing. This is called RNAi technology and has important applications in several fields. In this paper, we review RNAi mechanisms in insects as well as the potential of RNAi technology to contribute to species-specific insecticidal strategies. Regarding this aspect, we cover the range of strategies considered and investigated so far, as well as their limitations and the most promising approaches to overcome them. Additionally, we discuss patterns of viral infection, specifically persistent and acute insect viral infections. In the latter case, we focus on infections affecting economically relevant species. Within this scope, we review the use of insect-specific viruses as bio-insecticides. Last, we discuss RNAi-based strategies to protect beneficial insects from harmful viral infections and their potential practical application. As a whole, this manuscript stresses the impact of insect viruses and RNAi technology in human life, highlighting clear lines of investigation within an exciting and promising field of research.

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“…Overexpression of Ago2 and Dicer2 can improve the susceptibility of silkworm to dsRNA (31). Expression of dsRNA targeting the viral genes of BmNPV (13), BmCPV (18), and BmBDV (20) in transgenic silkworms substantially decreased the viral mRNA content and silkworm mortality after viral infection. The siRNA pathway is the predominant mechanism responsible for antiviral activity in insects (27,28).…”

Section: Rnai Pathwaysmentioning

confidence: 99%

“…The BmN and BmE are two cell lines commonly used in silkworm research, which are derived from the ovary and embryonic cells of silkworm, respectively. The BmNPV can infect the two cell lines, unlike the BmCPV and BmBDV; therefore, most silkworm antiviral research is focused on the BmNPV (11)(12)(13)(14)(15)(16)(17), a few on the BmCPV (18,19), and very few on the BmBDV (20).…”

Section: Characteristics Of Silkworm Virusesmentioning

confidence: 99%

Insights Into the Antiviral Pathways of the Silkworm Bombyx mori

Jiang

2021

Front. Immunol.

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The lepidopteran model silkworm, Bombyx mori, is an important economic insect. Viruses cause serious economic losses in sericulture; thus, the economic importance of these viruses heightens the need to understand the antiviral pathways of silkworm to develop antiviral strategies. Insect innate immunity pathways play a critical role in the outcome of infection. The RNA interference (RNAi), NF-kB-mediated, immune deficiency (Imd), and stimulator of interferon gene (STING) pathways, and Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway are the major antiviral defense mechanisms, and these have been shown to play important roles in the antiviral immunity of silkworms. In contrast, viruses can modulate the prophenol oxidase (PPO), phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt), and the extracellular signal-regulated kinase (ERK) signaling pathways of the host to elevate their proliferation in silkworms. In this review, we present an overview of the current understanding of the main immune pathways in response to viruses and the signaling pathways modulated by viruses in silkworms. Elucidation of these pathways involved in the antiviral mechanism of silkworms furnishes a theoretical basis for the enhancement of virus resistance in economic insects, such as upregulating antiviral immune pathways through transgenic overexpression, RNAi of virus genes, and targeting these virus-modulated pathways by gene editing or inhibitors.

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Enhancement of antiviral capacity of transgenic silkworms against cytoplasmic polyhedrosis virus via knockdown of multiple viral genes

Cited by 18 publications

References 10 publications

Advances in the Arms Race Between Silkworm and Baculovirus

Advances in the Arms Race Between Silkworm and Baculovirus

RNA Interference in Insects: Protecting Beneficials and Controlling Pests

Insights Into the Antiviral Pathways of the Silkworm Bombyx mori

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