“…The sequences of the primers used were as follows: ICP0 forward, 5 0 -GACAGCAAAAATCCCCTGAG-3 0 ; ICP 0 reverse, 5 0 -ACGAGGGAAAACAATAAGGG-3 0 ; ICP6 forward, 5 0 -GACAGCCATATCCTGAGC-3 0 ; ICP6 reverse, 5 0 -ACTCACAGATCGTTGACGACCG-3 0 ; TK forward, 5 0 -ATACCGACGATATGCGACCT-3 0 ; TK reverse, 5 0 -TTATTGCCGTCATAGCGCGG-3 0 ; gD forward, 5 0 -ATGGGAGGCAACTGTGCTAT-3 0 ; gD reverse, 5 0 -CTCGGTGCTCCAGGATAAAC-3 0 ; LacZ forward, 5 0 -GCGTTACCCAACTTAATCG-3 0 ; and LacZ reverse, 5 0 -TGTGAGCGAGTAACAACC-3 0 ; b-actin forward, 5 0 -GTGGGCCGCTCTAGGCACCAA-3 0 ; and bactin reverse, 5 0 -CTCTTTGATGTCACGCACGATTTC-3 0 . 9 To confirm the integrity of each RNA sample, a PCR analysis of the b-actin gene was performed. The PCR amplification of cDNAs was carried out in volumes of 50 ml for 30 cycles at a denaturing temperature of 94 1C for 30 s, an annealing temperature of 60 1C for 30 s and an extension temperature of 72 1C for 2 min using a Gene Amp PCR system 9700 (Applied Biosystems, Foster City, CA).…”