Enhancement of antiproliferative activity by molecular simplification of catalpol

García, Celina; León, Leticia G.; Pungitore, Carlos Rodolfo; Rios‐Luci, Carla; Daranas, Antonio Hernández; Montero, Juan Carlos; Pandiella, Atanasio; Tonn, Carlos E.; Martı́n, Victor S.; Padrón, José M.

doi:10.1016/j.bmc.2010.02.044

Cited by 19 publications

(10 citation statements)

References 20 publications

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“…Catalpol is one of the key active ingredients of Rehmannia, which has effects on the nervous and cardiovascular systems, in addition to exhibiting antihyperglycemic, antitumor and antiproliferative activities (17)(18)(19). However, previous studies have only demonstrated that catalpol is able to reproduce the diuretic, laxative and hypoglycemic effects of Rehmannia (20). The present study aimed to investigate whether catalpol inhibited cancer growth by upregulating microRNA-146a and downregulating MMP-16 expression.…”

Section: Introductionmentioning

confidence: 92%

Catalpol suppresses proliferation and facilitates apoptosis of MCF-7 breast cancer cells through upregulating microRNA-146a and downregulating matrix metalloproteinase-16 expression

Liu

et al. 2015

Molecular Medicine Reports

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Breast cancer is the most common malignancy in women, and was the second highest cause of mortality in women in 2013. Matrix metalloproteinase-16 (MMP-16) is an enhancer of the invasion of breast cancer cells. The expression of microRNA‑146a (miR‑146a) has been reported to be significantly greater in patients with breast cancer compared with healthy controls. Catalpol is one of the main active ingredients of Rehmannia, of which the key pharmacological effects are antitumoral and antiproliferative. The present study was performed to investigate the effect of catalpol on breast cancer and to explore the potential therapeutic mechanisms. Cell proliferation was investigated using an MTT assay, and caspase‑3 activity assays and flow cytometry were used to assess apoptosis in MCF‑7 breast cancer cells. The expression levels of MMP‑16 and miR‑146a were investigated using gelatin zymography assays and reverse transcription-quantitative polymerase chain reaction, respectively. In addition, MCF‑7 cells were transfected with miR‑146a and anti‑miR‑146a to further investigate the effects of catalpol. The results demonstrated that catalpol reduced proliferation and promoted apoptosis in MCF‑7 cells, reducing MMP‑16 activity and increasing the expression of miR‑146a in MCF‑7 cells. This suggests that miR‑146a may regulate and control the expression levels of MMP‑16 in MCF‑7 cells. In conclusion, catalpol suppresses proliferation and facilitates apoptosis of MCF‑7 breast cancer cells through upregulating miR‑146a and downregulating MMP-16 expression.

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Section: Introductionmentioning

confidence: 92%

Catalpol suppresses proliferation and facilitates apoptosis of MCF-7 breast cancer cells through upregulating microRNA-146a and downregulating matrix metalloproteinase-16 expression

Liu

et al. 2015

Molecular Medicine Reports

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“…As shown in Table , the content of each compound varied markedly dependent on the production places, for example, the content of the new compound S4 varied from 0.128 to 0.799 mg/g in the six samples. Among them, catalpol and aucubin ( S3 and S6 ) are generally considered the highly active ingredients, especially for anti‐cancer activity (Garcia, Leon, & Pungitore, ; Lv et al, ; Pungitore, Ayub, & Borkowski, ; Pungitore, Leon, & Garcia, ; Wang et al, ). Catapol was the richest among six compounds and its average content in the samples from six regions was 1.245 mg/g.…”

Section: Resultsmentioning

confidence: 99%

HPLC analysis of six iridoid glycosides from Cymbaria dahurica L. and their structural elucidation

et al. 2017

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Studies on ethanol extract from Cymbaria dahurica L. (C. dahurica) resulted in isolation and identification of a new iridoid glycoside together with five known compounds. Moreover, a highperformance liquid chromatographic method has been developed for the simultaneous determination of six iridoid glycosides in C. dahurica. The separation by gradient elution was performed on Inertsil ODS-SP column (250 mm 3 4.6 mm, 5 mm) at 308C with acetonitrile and water as the mobile phase, and monitored by absorbance at 210 nm. The recovery of the method is from 95.0 to 98.0%, and linearity (r > .9994) was obtained for six compounds. A high degree of specificity as well as repeatability and reproducibility (relative standard deviation values less than 2.6%) were also achieved. The validated method was successfully applied to the determination of six compounds in six samples. Practical applicationsThe aerial parts of Cymbaria dahurica has been used as foods and herbal remedies in Inner Mongolia of China, which has a wide range of health benefits, significantly antioxidant properties. In previous phytochemical studies, non-glycosidic iridoids and flavonoids were isolated. Iridoids are a class of secondary metabolites with the significant pharmacological activities including antitumor, antioxidant, and anti-inflammatory. Therefore, a detailed analysis and recognition of structural compounds diversity in C. dahurica is of great importance to evaluate their biological activities. K E Y W O R D SCymbaria dahurica L, cymdahoside A, HPLC, iridoid glycoside

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“…[7,8] Furthermore, we have previously synthesized the bicyclic aglycone derivatives by means of a cyclization reaction catalyzed by L-proline. [9] These compounds represent a simplified scaffold of the aglycone framework of naturally occurring iridoids, and their silylated derivatives showed remarkable biological activity towards human cancer cell lines, including cell cycle arrest and apoptosis induction. Therefore, they could be used as therapeutic compounds for treatment of cancer, either alone or in combination.…”

Section: Mass Spectrometry Studies Of Iridoid Aglycone Derivativesmentioning

confidence: 99%

“…The compound studied in this paper was synthesized from β-citronellol and the methodology was inspired by the total synthesis of brasoside and littoralisone realized by Mangion and MacMillan. [9,14] Compound 2 was a clear and colorless oil, which exhibited a molecular ion at m/z 450 corresponding to the molecular formula C 27 H 34 O 4 Si. The EI-HRMS measurements were performed on a Micromass VG AutoSpec (Manchester UK), at the Instituto Universitario de Bioorgánica (Universidad de La Laguna), at a resolution of 5000 (5% valley definition), by 70 eV electron ionization, at an accelerating voltage of 8 kV.…”

Section: Mass Spectrometry Studies Of Iridoid Aglycone Derivativesmentioning

confidence: 99%

Mass spectrometry studies of iridoid aglycone derivatives

Pungitore

García

Martı́n

et al. 2011

Rapid Comm Mass Spectrometry

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Enhancement of antiproliferative activity by molecular simplification of catalpol

Cited by 19 publications

References 20 publications

Catalpol suppresses proliferation and facilitates apoptosis of MCF-7 breast cancer cells through upregulating microRNA-146a and downregulating matrix metalloproteinase-16 expression

Catalpol suppresses proliferation and facilitates apoptosis of MCF-7 breast cancer cells through upregulating microRNA-146a and downregulating matrix metalloproteinase-16 expression

HPLC analysis of six iridoid glycosides from Cymbaria dahurica L. and their structural elucidation

Mass spectrometry studies of iridoid aglycone derivatives

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