Enhancement of Antibody Binding on SiO₂ Love Wave Sensor Surface using (3-glycidoxypropyl)trimethoxysilane

Abstract

An acoustic Love wave immunosensor dedicated to detect biological species such as bacteria, viruses or toxins is described in this paper. We present and analyze results of antibody grafting on the Love wave device using GPTS as coupling agent between SiO 2 sensor surface and the sensitive biomembrane layer composed of antibodies. Goat anti-mouse (GAM) antibodies at concentrations from 2µg/ml to 50 µg/ml were introduced on the sensor brass cell. The saturation of the sensor surface by antibodies appears at a concentration of 20 µg/ml. The detection threshold of this primary antibody is around 2 µg/ml, and the lowest concentration allowing antigen detection is around 5 µg/ml. Mater. Res. Soc. Symp. Proc. Vol. 915