Enhancement of Angiogenesis and Epithelialization Processes in Mice with Burn Wounds through ROS/RNS Signals Generated by Non‐Thermal N<sub>2</sub>/Ar Micro‐Plasma

Thi, Minh Hien Ngo; Shao, Pei‐Lin; Liao, Jiunn Der; Lin, Chou Ching K.; Yip, Hon Kan

doi:10.1002/ppap.201400072

Cited by 46 publications

(30 citation statements)

References 28 publications

(38 reference statements)

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“…Enhanced wound healing was also shown in numerous animal studies [Garcia-Alcantara et al, 2013;Shao et al, 2016;Schmidt et al, 2017b]. Cold plasmaderived ROS/RNS are hypothesized to play a major role in these processes [Arndt et al, 2013a;Ngo Thi et al, 2014;Xu et al, 2015]. Plasma research underlines the importance of redox control of wound healing, a concept long known in the field of redox biology [Sen, 2003;Sen and Roy, 2008;Sen, 2009].…”

Section: Introductionmentioning

confidence: 90%

“…All mentioned components can be tailored by the operating parameters such as voltage, current, gas, and geometry. Cold plasmaderived ROS/RNS are hypothesized to play a major role in these processes [Arndt et al, 2013a;Ngo Thi et al, 2014;Xu et al, 2015]. Three plasma devices have received accreditation as medical device based on clinical observations with chronic wounds.…”

Section: Introductionmentioning

confidence: 99%

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High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

Bekeschus

Schmidt

Krämer

et al. 2018

Environ and Mol Mutagen

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Promising cold physical plasma sources have been developed in the field of plasma medicine. An important prerequisite to their clinical use is lack of genotoxic effects in cells. During optimization of one or even different plasma sources for a specific application, large numbers of samples need to be analyzed. There are soft and easy-to-assess markers for genotoxic stress such as phosphorylation of histone H2AX (γH2AX) but only few tests are accredited by the OECD with regard to mutagenicity detection. The micronucleus (MN) assay is among them but often requires manual counting of many thousands of cells per sample under the microscope. A high-throughput MN assay is presented using image flow cytometry and image analysis software. A human lymphocyte cell line was treated with plasma generated with ten different feed gas conditions corresponding to distinct reactive species patterns that were investigated for their genotoxic potential. Several millions of cells were automatically analyzed by a MN quantification strategy outlined in detail in this work. Our data demonstrates the absence of newly formed MN in any feed gas condition using the atmospheric pressure plasma jet kINPen. As positive control, ionizing radiation gave a significant 5-fold increase in micronucleus frequency. Thus, this assay is suitable to assess the genotoxic potential in large sample sets of cells exposed chemical or physical agents including plasmas in an efficient, reliable, and semiautomated manner. Environ. Mol. Mutagen. 59:268-277, 2018. © 2018 Wiley Periodicals, Inc.

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Section: Introductionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

Bekeschus

Schmidt

Krämer

et al. 2018

Environ and Mol Mutagen

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show abstract

Section: Introductionmentioning

confidence: 97%

“…Currently, there are some methods used for the skin examination. Optical coherence tomography (OCT) and histology with the haematoxylin and eosin (H&E) staining are the common techniques for the assessment of sectioned tissue in tissue fields (Mayrovitz & Soontupe, 2009;Ngo Thi et al, 2014). Shao et al (2016) selected these two techniques to evaluate of the effect of microplasma on the wounded skin and demonstrated the acceleration of wound recovery.…”

Section: Introductionmentioning

confidence: 99%

Structure‐dependent behaviours of skin layers studied by atomic force microscopy

Chang

Liu

Shao

et al. 2017

Journal of Microscopy

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The multilayer skin provides the physical resistance and strength against the environmental attacks, and consequently plays a significant role in maintaining the mammalian health. Currently, optical microscopy (OM) is the most common method for the research related to skin tissues while with the drawbacks including the possibility of changing the native morphology of the sample with the addition of the chemical or immunological staining and the restricted resolution of images for the direct observation of the tissue structures. To investigate if the function of each tissue is structure-dependent and the how the injured skin returns to the intact condition, we applied atomic force microscopy (AFM) on the sectioned mice-skin to reveal the tissue structures with a nanoscale resolution. From the outermost stratum to the inner layer of the skin tissue, the respectively laminated, fibrous, and brick-like structures were observed and corresponded to various functions. Due to the mechanical differences between the tissue constituents and their boundaries, the sizes and arrangements of the components were characterised and quantified by the mechanical mapping of AFM, which enabled the analytical comparisons between tissue layers. For the wound model, the skin tissues were examined with the initial formation of blood vessels and type-I collagen, which agreed with the stage of healing process estimated by OM but showed more detail information about the evolution of proteins among the skin. In conclusion, the characterisation of the components that consist of skin tissue by AFM enables the connection of the tissue function to the corresponded ultrastructure.

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“…4,7,13 For instance Thi et al reported that the high NO content in a cold N 2 /Ar microplasma stimulated angiogenesis and epithelialization and enhanced the healing of burn wounds in mice. 18 Different treatment effects on ACD in mice were observed here using an argon discharge with different gas impurities. Enhanced skin restoration and regeneration was only achieved by humid plasma-exposure.…”

Section: Discussionmentioning

confidence: 79%

Surface treatment with non-thermal humid argon plasma as a treatment for allergic contact dermatitis in a mouse model

Xiong

Wang

Yin

et al. 2018

Clinical Plasma Medicine

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Cold plasma generated at atmospheric pressure has attracted intense interest in biomedical applications, particularly as an antimicrobial treatment. Here we report the therapeutic effect of humidified cold argon plasma on allergic contact dermatitis (ACD) in a mouse model. Treatment was carried out with different gas compositions: argon gas containing small amounts of either N2, O2, or H2O. The best effect was obtained using humid plasma (H2O addition), where the ACD symptoms decreased after one or two 1-min plasma treatments. Even for severe ACD with ulcers and crust formation, the humid plasma-treated mice recovered faster than the control group. Histopathological analysis by H&E-staining showed enhanced epithelialization with formation of collagen and hair follicles in the affected skin after humid plasma exposure. The therapeutic ability of the humid argon plasma discharge was proposed to be induced by reactive oxygen species (HxOy) transported from the discharge zone, which are adhesive and accumulate on the skin surface, penetrating the subcutis to eliminate inflammation. However, in treatments using plasma with addition of oxygen or nitrogen (without water) the active gaseous species are blocked due to poor adhesion to and penetration into the dry ACD skin, with correspondingly poor treatment effects. The enhanced in vivo healing in ACD mice indicate the non-thermal humid plasma could be a potential alternative approach for therapy of ACD and other inflammatory skin diseases.

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Enhancement of Angiogenesis and Epithelialization Processes in Mice with Burn Wounds through ROS/RNS Signals Generated by Non‐Thermal N₂/Ar Micro‐Plasma

Cited by 46 publications

References 28 publications

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

Structure‐dependent behaviours of skin layers studied by atomic force microscopy

Surface treatment with non-thermal humid argon plasma as a treatment for allergic contact dermatitis in a mouse model

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Enhancement of Angiogenesis and Epithelialization Processes in Mice with Burn Wounds through ROS/RNS Signals Generated by Non‐Thermal N2/Ar Micro‐Plasma

Cited by 46 publications

References 28 publications

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

High throughput image cytometry micronucleus assay to investigate the presence or absence of mutagenic effects of cold physical plasma

Structure‐dependent behaviours of skin layers studied by atomic force microscopy

Surface treatment with non-thermal humid argon plasma as a treatment for allergic contact dermatitis in a mouse model

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Enhancement of Angiogenesis and Epithelialization Processes in Mice with Burn Wounds through ROS/RNS Signals Generated by Non‐Thermal N₂/Ar Micro‐Plasma