Enhancement of analyte detectability in isotachophoresis exploiting spectrophotometric diode‐array detection with chemometric data processing

Abstract

The potentialities of capillary ITP combined with diode-array detection (DAD) with subsequent chemometric data processing have been investigated in this work. A series of different migration configurations were created using model analytes, interferents and appropriate spacers. Special attention has been paid not only to constituents migrating in fully developed ITP zones but also to the spike mode of ITP migration. The purity assessment and identity confirmation of model analytes migrating in both modes were performed by means of multivariate curve resolution and target transformation factor analysis (TTFA). Their successful applications have revealed a smart way to increase in the analytical information obtained by ITP separation even in the instance of trace analysis.

Liquid chromatography with diode array detection combined with spectral deconvolution for the analysis of some diterpene esters in Arabica coffee brew

“…DAD, due to its low price and good precision, is a commonly hyphenated detector but its low spectral selectivity does not often allow the resolution of coelution problems as easily as with a MS detector. The application of chemometric approaches in conjunction with the DAD dataset allows the mathematical separation of coeluted species , where mathematical separation refers to the deconvolution of the dataset to a series of chromatograms by mathematical means.…”

Recent progress in analytical capillary isotachophoresis

“…Another article 27 investigated the effect of ionic strength on analyte' preconcentration in peak mode ITP and confirmed its particular impact on separations with multivalent ionic analytes and spacers. The possibilities of a chemometric approach to DAD‐ITP data were investigated for various migration configurations of analytes, interferents, and spacers both for regular and for spike mode ITP zones 28. It was shown that purity assessment and identity confirmation by means of multivariate curve resolution and target transformation factor analysis has the potential to increase the analytical output even in the case of trace analysis.…”