14In this manuscript, the separation of four kahweol esters and four cafestol esters that are present in 15Arabica coffee brews was investigated using HPLC/DAD. Those compounds could not be baseline 16 separated in a single chromatogram using RP-LC but the kahweol esters could be selectively detected by 17 setting the wavelength at 290 nm. In this case the four kahweol esters were baseline separated allowing 18 for their quantification. Such approach was not possible for the cafestol esters and spectral deconvolution 19 was used to obtain deconvolved chromatograms that were specific to cafestol and kahweol esters 20 respectively. In each of those chromatograms, the 4 esters were baseline separated allowing for the 21 quantification of the eight targeted compounds. Because kahweol esters could be quantified either using 22 the chromatogram obtained by setting the wavelength at 290 nm or using the deconvolved chromatogram, 23 those compounds were used to compare the analytical performances. Slightly better LOD were obtained 24 using the deconvolved chromatogram (average LOD of 5.7 mg/L against 6.7 mg/L). Identical 25 concentrations were found in a real sample with both approaches. The peak areas of the different diterpene 26 esters in the deconvolved chromatograms were proven to be repeatable with an average intra-day 27 repeatability of 0.8 % (6 replicates) and an inter-day repeatability of 1.0% (3 successive days, two 28 replicates each day). This work demonstrates the accuracy of spectral deconvolution in conjunction with 29 HPLC-DAD (HPLC-DAD/SD) to mathematically separate co-eluting compounds using the full spectra 30 recorded by the DAD. 31 32