Enhancement effect of hematite and nickel nanoparticles on biohydrogen production from dairy wastewater

Influence of nickel and hematite nanoparticle powder on the production of biohydrogen from complex distillery wastewater in batch fermentation

“…The XRD pattern of the samples indicated the cubic phase of NiO nanoparticles (JCPDS card file number: 4-0835) (Fig. 1A) and rhombohedral phase of Fe 2 O 3 nanoparticles (JCPDS card No: 01-084-0307), respectively [9,15,18,19] (Fig. 1B).…”

“…The average size of NiO and Fe 2 O 3 NPs was calculated using the Scherrer formula as described elsewhere [9,16,19]. The average size for nickel oxide and hematite nanoparticles was: 23 nm and 33 nm, respectively.…”

“…5 Pathway 2). The [FeFe] hydrogenase enzyme is widely distributed among the various hydrogen producing microbes compared to [NiFe] hydrogenase [9]. Furthermore, the enzyme activity of [FeFe] hydrogenase has also been reported substantially higher compared to [NiFe] hydrogenase [33].…”

“…Recently, an application of nanotechnology for an enhancement of process efficiency by enhancing bioactivity of hydrogen producing microbes has gained enormous attention. The Fe and Ni nanoparticles (NP's) have been reported to enhance an activity of ferredoxin oxidoreductase by increasing electron transfer rate, which in turn enhances an activity of NADH dependent [FeFe] hydrogenase /or [NiFe] hydrogenase or indirectly by ferredoxin dependent hydrogenase, and therefore, results in an enhanced HY [9]. An extensive literature survey shows that an application of Fe and Ni NP's to enhance biohydrogen production from various pure carbon sources viz.…”

Biohydrogen Production from Wastewaters

“…The addition of iron supplement was shown to improve the hydrogen production. Gadhe et al [24] demonstrated the efects of nano-sized iron and nickel oxide nanoparticles by using dairy wastewater as a substrate, and it showed that an enhancement in hydrogen yield of 17.2 mmol/g COD is due to the enhanced activity of the ferredoxin oxidoreductase, ferredoxin and hydrogenase enzymes. Moreover, the optimal value for the Fe 2+ concentration is varied with the type of substrates used.…”