Enhanced β-carotene production in<i>Yarrowia lipolytica</i>through the metabolic and fermentation engineering

Jing, Yiwen; Wang, Jingnan; Gao, Haiyan; Jiang, Yujia; Zhang, Wenming; Jiang, Min; Xin, Fengxue; Zhang, Wenming

doi:10.1093/jimb/kuad009

Cited by 6 publications

(3 citation statements)

References 44 publications

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“…Another contributing factor is the inappropriate concentration of residual glucose in the fermenter because one UDPG derived from glucose is required to produce one gastrodin (Figure ). In the future, improving the fermentation efficiency will involve optimizing the fermentation process, including medium and fed-batch optimization, through expert operation and artificial intelligence strategies. , …”

Section: Resultsmentioning

confidence: 99%

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Wu,

Li,

Sun

et al. 2024

ACS Synth. Biol.

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Gastrodin, 4-hydroxybenzyl alcohol-4-O-β-D-glucopyranoside, has been widely used in the treatment of neurogenic and cardiovascular diseases. Currently, gastrodin biosynthesis is being achieved in model microorganisms. However, the production levels are insufficient for industrial applications. In this study, we successfully engineered a Yarrowia lipolytica strain to overproduce gastrodin through metabolic engineering. Initially, the engineered strain expressing the heterologous gastrodin biosynthetic pathway, which comprises chorismate lyase, carboxylic acid reductase, phosphopantetheinyl transferase, endogenous alcohol dehydrogenases, and a UDP-glucosyltransferase, produced 1.05 g/L gastrodin from glucose in a shaking flask. Then, the production was further enhanced to 6.68 g/L with a productivity of 2.23 g/L/day by overexpressing the key node DAHP synthases of the shikimate pathway and alleviating the native tryptophan and phenylalanine biosynthetic pathways. Finally, the best strain, Gd07, produced 13.22 g/L gastrodin in a 5 L fermenter. This represents the highest reported production of gastrodin in an engineered microorganism to date, marking the first successful de novo production of gastrodin using Y. lipolytica.

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Section: Resultsmentioning

confidence: 99%

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Wu,

Li,

Sun

et al. 2024

ACS Synth. Biol.

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“…β-carotene is a kind of high-value tetraterpene compound, which shows various applications in medical, agricultural and industrial areas owing to its antioxidant, antitumor and anti-inflammatory activities. The strain obtained (Y. lipolytica-C (Yli-C)) reached 34.5 mg/L β-carotene [283].…”

Section: Increase Of Gene Copy Numbermentioning

confidence: 99%

Strategies for the Development of Industrial Fungal Producing Strains

Salazar-Cerezo

Vries

Garrigues

2023

JoF

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The use of microorganisms in industry has enabled the (over)production of various compounds (e.g., primary and secondary metabolites, proteins and enzymes) that are relevant for the production of antibiotics, food, beverages, cosmetics, chemicals and biofuels, among others. Industrial strains are commonly obtained by conventional (non-GMO) strain improvement strategies and random screening and selection. However, recombinant DNA technology has made it possible to improve microbial strains by adding, deleting or modifying specific genes. Techniques such as genetic engineering and genome editing are contributing to the development of industrial production strains. Nevertheless, there is still significant room for further strain improvement. In this review, we will focus on classical and recent methods, tools and technologies used for the development of fungal production strains with the potential to be applied at an industrial scale. Additionally, the use of functional genomics, transcriptomics, proteomics and metabolomics together with the implementation of genetic manipulation techniques and expression tools will be discussed.

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“…By further increasing the copies of genes related to β-carotene synthesis, production was boosted in the engineered strain up to 117.5 mg/L. Finally, they were able to produce 2695.5 mg/L of β-carotene in fed-batch fermentation by using a 5 L bioreactor [ 134 ].…”

Section: Recent Trends In Biotechnological Production Of Fungal Carot...mentioning

confidence: 99%

Industrially Important Fungal Carotenoids: Advancements in Biotechnological Production and Extraction

Naz

Ullah

Nazir

et al. 2023

JoF

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Carotenoids are lipid-soluble compounds that are present in nature, including plants and microorganisms such as fungi, certain bacteria, and algae. In fungi, they are widely present in almost all taxonomic classifications. Fungal carotenoids have gained special attention due to their biochemistry and the genetics of their synthetic pathway. The antioxidant potential of carotenoids may help fungi survive longer in their natural environment. Carotenoids may be produced in greater quantities using biotechnological methods than by chemical synthesis or plant extraction. The initial focus of this review is on industrially important carotenoids in the most advanced fungal and yeast strains, with a brief description of their taxonomic classification. Biotechnology has long been regarded as the most suitable alternative way of producing natural pigment from microbes due to their immense capacity to accumulate these pigments. So, this review mainly presents the recent progress in the genetic modification of native and non-native producers to modify the carotenoid biosynthetic pathway for enhanced carotenoid production, as well as factors affecting carotenoid biosynthesis in fungal strains and yeast, and proposes various extraction methods to obtain high yields of carotenoids in an attempt to find suitable greener extraction methods. Finally, a brief description of the challenges regarding the commercialization of these fungal carotenoids and the solution is also given.

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Enhanced β-carotene production inYarrowia lipolyticathrough the metabolic and fermentation engineering

Cited by 6 publications

References 44 publications

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Strategies for the Development of Industrial Fungal Producing Strains

Industrially Important Fungal Carotenoids: Advancements in Biotechnological Production and Extraction

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