Enhanced β-carotene and Biomass Production by Induced Mixotrophy in Dunaliella salina across a Combined Strategy of Glycerol, Salinity, and Light

Capa-Robles, Willian; García‐Mendoza, Ernesto; Paniagua‐Michel, J.

doi:10.3390/metabo11120866

Cited by 9 publications

(4 citation statements)

References 70 publications

(96 reference statements)

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“…Thus, in order to increase the economic efficiency of the heterotrophic bio-oil production technology using microalgae, other, cheaper sources of carbon should be used. One of the proposed solutions is waste technical glycerin, which allows a high final concentration of microalgae biomass and desired bioproducts to be obtained [ 32 , 33 ]. Rattanapoltee et al (2021) confirmed the marginal influence of impurities in raw technical glycerin, e.g., soap, methanol, on the final concentration of biomass and the final content of valuable fermentation products such as DHA [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Optimization of Lipid Production by Schizochytrium limacinum Biomass Modified with Ethyl Methane Sulfonate and Grown on Waste Glycerol

Talbierz¹,

Dębowski

Kujawska³

et al. 2022

IJERPH

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One of the most promising avenues of biofuel research relates to using waste as a starting feedstock to produce liquid or gaseous energy carriers. The global production of waste glycerol by the refinery industry is rising year after year. The aim of the present study was to examine the effect of ethyl methane sulfonate (EMS) on the growth rates and intracellular lipid accumulation in heterotrophically-cultured Schizochytrium limacinum microalgae, grown on waste glycerol as the carbon source. The strain S. limacinum E20, produced by incubating a reference strain in EMS for 20 min, was found to perform the best in terms of producing biomass (0.054 gDW/dm3·h) and accumulating intracellular bio-oil (0.021 g/dm3·h). The selected parameters proved to be optimal for S. limacinum E20 biomass growth at the following values: temperature 27.3 °C, glycerol level 249.0 g/dm3, oxygen in the culture 26%, and yeast extract concentration 45.0 g/dm3. In turn, the optimal values for lipid production in an S. limacinum E20 culture were: temperature 24.2 °C, glycerol level 223.0 g/dm3, oxygen in the culture 10%, and yeast extract concentration 10.0 g/dm3. As the process conditions are different for biomass growth and for intracellular lipid accumulation, it is recommended to use a two-step culture process, which resulted in a lipid synthesis rate of 0.41 g/dm3·h.

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Section: Resultsmentioning

confidence: 99%

Optimization of Lipid Production by Schizochytrium limacinum Biomass Modified with Ethyl Methane Sulfonate and Grown on Waste Glycerol

Talbierz¹,

Dębowski

Kujawska³

et al. 2022

IJERPH

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“…The transcriptome analysis conducted by Li et al (2020) also showed that red light is most suiTablele for algal growth, with stronger expression of growth-control genes compared to blue light. In contrast, cellular carotenoid content as well as the expression of reactive oxygen species (ROS) synthase genes were enhanced under blue-spectrum illumination [13].…”

Section: Discussionmentioning

confidence: 99%

“…With regards to culture medium condition, many studies have been conducted in an effort to find out the optimal condition of culture that stimulates the highest production of biomass and β-carotene, that mostly focused on nutritional factors (i.e., composition and content) and major environmental factors (i.e., salinity, temperature, pH of the culture medium and light intensity) [10][11][12]. Capa-Robles et al (2021) reported that a combination of 12.5 mM glycerol, 3.0 M salinity, and 50 μmol photons m −2 s −1 light intensity resulted in the best growth of 2.1 × 10 6 cell mL ) of the Mexican strain, D. salina BC02 [13]. In the study of Mohammadi et al (2023), the optimal condition for the growth of D. salina was at 3 molars salinity, pH higher than 7, nitrate concentration of 0.25 g/L, light intensity of 5000 lux, and temperature of 25 degrees Celsius, whereas the optimum β-carotene production occurred at a similar condition of these parameters but in a temperature of 30 degrees Celsius [14].…”

Section: Introductionmentioning

confidence: 99%

Effects of Light on the Growth and β-carotene Accumulation in the Green Algae Dunaliella salina

Tran-Nguyen

Tran

Trinh-Dang

2023

AJOB

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The marine microalgae Dunaliella salina holds significant economic value due to its rich content of β-carotene, a natural pigment with high antioxidant capacity, immune-stimulating properties, and a crucial role in antioxidant reactions with vitamins C and E. This study evaluated the effects of light factors on the growth and biosynthesis of β-carotene in the Dunaliella salina strain isolated from coastal waters in Central Vietnam. The microalgae strain was cultured in the static f/2 medium at 25°C, under different light spectra (blue, red, and white) and light intensities (13.5, 27.0, and 40.5 µmol photon/m2/s), with a similar light/dark cycle of 16/8. The results indicated that the microalgae strain showed the highest growth rate and production yield of β-carotene under the red LED light, whereas the highest β-carotene accumulation in each microalgal cell was obtained under the blue LED light. Regarding light intensity, the best growth and β-carotene production yield was observed under the highest experimental light intensity of 40.5 µmol photon/m2/s, but the highest β-carotene content per algal cell was reached under the lowest intensity (13.5 µmol photon/m2/s). These findings provide important scientific data for further research to identify the optimal conditions for increasing biomass and stimulating the accumulation of valuable secondary compounds of the microalgae Dunaliella salina in Vietnam.

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“…1). The reason might be that the osmotic pressure modification in the culture medium along with low photon flux density stressed the microalgae, triggering lipid production and limiting biomass production (Capa-Robles et al 2021).…”

Section: Total Lipidsmentioning

confidence: 99%

Fatty acid profile and productivity variation during the growth of Dunaliella sp. under different photon flux densities and glycerol concentrations

Alva¹,

Noreña-Barroso²,

Guerra‐Castro³

et al. 2022

Lat. Am. J. Aquat. Res.

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Microalgae can accumulate lipids during the stationary growth phase, but little information is available about fatty acid profile changes during this phase to determine the best harvesting time in respect to lipid content. In this study, Dunaliella sp. was cultured in F/2 medium at three different photon flux densities (380, 226 and 8.2 μmol photon m-2 s-1) and three different glycerol concentrations (0, 10 and 20 g L-1). Samples were taken during the stationary phase to assess lipid content and fatty acid profile variations. Microalgal biomass production was higher at 380 and 226 μmol photon m-2 s-1 than at 8.2 μmol photon m-2 s-1 in accord to light limitation. The maximum lipid content (345.78 mg g-1) was achieved at 8.2 μmol photon m-2 s-1 and 20 g L-1 glycerol at day 12, similar to that achieved at day 9 (334.16 mg g-1). The maximum polyunsaturated fatty acid amount (65.30 μg mg-1) was achieved at day 7 of culture without glycerol addition, decreasing in proportion over time. So, the best conditions and harvesting time in respect to fatty acid quality would be at 380 μmol photon m-2 s-1 without glycerol addition and after 7 days of culture.

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Enhanced β-carotene and Biomass Production by Induced Mixotrophy in Dunaliella salina across a Combined Strategy of Glycerol, Salinity, and Light

Cited by 9 publications

References 70 publications

Optimization of Lipid Production by Schizochytrium limacinum Biomass Modified with Ethyl Methane Sulfonate and Grown on Waste Glycerol

Optimization of Lipid Production by Schizochytrium limacinum Biomass Modified with Ethyl Methane Sulfonate and Grown on Waste Glycerol

Effects of Light on the Growth and β-carotene Accumulation in the Green Algae Dunaliella salina

Fatty acid profile and productivity variation during the growth of Dunaliella sp. under different photon flux densities and glycerol concentrations

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