Enhanced Trehalose Production Improves Growth of
            <i>Escherichia coli</i>
            under Osmotic Stress

Purvis, Jason E.; Yomano, Lorraine P.; Ingram, L. O.

doi:10.1128/aem.71.7.3761-3769.2005

Cited by 171 publications

(138 citation statements)

References 46 publications

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“…Zhou et al (2006) reported that d-())-lactate synthesis significantly increased when betaine was added to the culture medium as an osmoprotectant. It was likewise found that modification of the trehalose content in recombinant E. coli also resulted in higher growth (Purvis et al 2005), and trehalose-dependent stress tolerance was identified as a relevant trait for industrial purposes involving baker's yeast (Attfield 1997). A compatible solute like glycerol could serve not only as the main carbon source, but also as a relevant osmoprotectant, thus enhancing the behaviour of the ethanologenic strains.…”

Section: Discussionmentioning

confidence: 99%

Ethanol synthesis from glycerol by Escherichia coli redox mutants expressing adhE from Leuconostoc mesenteroides

Nikel

Ramírez

Pettinari

et al. 2010

Journal of Applied Microbiology

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Aims: Analysis of the physiology and metabolism of Escherichia coli arcA and creC mutants expressing a bifunctional alcohol‐acetaldehyde dehydrogenase from Leuconostoc mesenteroides growing on glycerol under oxygen‐restricted conditions. The effect of an ldhA mutation and different growth medium modifications was also assessed. Methods and Results: Expression of adhE in E. coli CT1061 [arcA creC(Con)] resulted in a 1·4‐fold enhancement in ethanol synthesis. Significant amounts of lactate were produced during micro‐oxic cultures and strain CT1061LE, in which fermentative lactate dehydrogenase was deleted, produced up to 6·5 ± 0·3 g l−1 ethanol in 48 h. Escherichia coli CT1061LE derivatives resistant to >25 g l−1 ethanol were obtained by metabolic evolution. Pyruvate and acetaldehyde addition significantly increased both biomass and ethanol concentrations, probably by overcoming acetyl‐coenzyme A (CoA) shortage. Yeast extract also promoted growth and ethanol synthesis, and this positive effect was mainly attributable to its vitamin content. Two‐stage bioreactor cultures were conducted in a minimal medium containing 100 μg l−1 calcium d‐pantothenate to evaluate oxic acetyl‐CoA synthesis followed by a switch into fermentative conditions. Ethanol reached 15·4 ± 0·9 g l−1 with a volumetric productivity of 0·34 ± 0·02 g l−1 h−1. Conclusions: Escherichia coli responded to adhE over‐expression by funnelling carbon and reducing equivalents into a highly reduced metabolite, ethanol. Acetyl‐CoA played a key role in micro‐oxic ethanol synthesis and growth. Significance and Impact of the Study: Insight into the micro‐oxic metabolism of E. coli growing on glycerol is essential for the development of efficient industrial processes for reduced biochemicals production from this substrate, with special relevance to biofuels synthesis.

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Section: Discussionmentioning

confidence: 99%

Ethanol synthesis from glycerol by Escherichia coli redox mutants expressing adhE from Leuconostoc mesenteroides

Nikel

Ramírez

Pettinari

et al. 2010

Journal of Applied Microbiology

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“…Since there are several reactions where UDP-glucose is used in biosynthesis [e.g. trehalose production in E. coli (Purvis et al, 2005)], perhaps some of these reactions are reversible enough in Aeromonas galU mutants to generate the low amount of UDP-glucose needed to introduce a single glucose residue into the LPS-core.…”

Section: Discussionmentioning

confidence: 99%

Mesophilic Aeromonas UDP-glucose pyrophosphorylase (GalU) mutants show two types of lipopolysaccharide structures and reduced virulence

et al. 2007

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A mutation in galU that causes the lack of O34-antigen lipopolysaccharide (LPS) in Aeromonas hydrophila strain AH-3 was identified. It was proved that A. hydrophila GalU is a UDP-glucose pyrophosphorylase responsible for synthesis of UDP-glucose from glucose 1-phosphate and UTP. The galU mutant from this strain showed two types of LPS structures, represented by two bands on LPS gels. The first one (slow-migrating band in gels) corresponds to a rough strain having the complete core, with two significant differences: it lacks the terminal galactose residue from the LPS-core and 4-amino-4-deoxyarabinose residues from phosphate groups in lipid A. The second one (fast-migrating band in gels) corresponds to a deeply truncated structure with the LPS-core restricted to one 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) and three L-glycero-Dmanno-heptose residues. galU mutants in several motile mesophilic Aeromonas strains from serotypes O1, O2, O11, O18, O21 and O44 were also devoid of the O-antigen LPS. The galU mutation reduced to less than 1 % the survival of these Aeromonas strains in serum, decreased the ability of these strains to adhere and reduced by 1.5 or 2 log units the virulence of Aeromonas serotype O34 strains in a septicaemia model in either fish or mice. All the changes observed in the galU mutants were rescued by the introduction of the corresponding single wild-type gene.

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“…In addition, carbohydrates may act as signaling molecules (Hanson and Smeekens 2009) and play a role in adaptive mechanisms to stress (Ramel et al 2009). Moreover, trehalose, a rare, non-reducing disaccharide, the presence of which was multiple times related to stress tolerance in bacteria (Purvis et al 2005) and fungi (Cao et al 2008), also accumulates in plants and seems to play a protective role during abiotic stress; however, its specific function is not well understood. In general, trehalose is not accumulated at high levels and, except some resurrection plants, it is barely detectable in crop plants (Fernandez et al 2010).…”

Section: Carbohydratesmentioning

confidence: 99%

Influence of abiotic stresses on plant proteome and metabolome changes

et al. 2013

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Plant responses to abiotic stresses are very complex phenomena with individual characteristics for various species. Abiotic stresses (e.g. drought, salinity, flooding, cold, heat, UV radiation, heavy metals, etc.) strongly affect plant growth and development. It is estimated that they are the cause of more than 50 % of crop yield losses. Abiotic stresses are known to activate a multigene response resulting in the changes in various proteins and primary and secondary metabolite accumulation. Therefore, proteomic and metabolomic approaches are becoming very important and powerful tools used in studying plants' reaction to various stimuli. Precise analysis of proteome and metabolome is essential for understanding the fundamentals of stress physiology and biochemistry. In this review, we focus on recent reports concerned to the influence of abiotic stresses on changes in the level of different protein groups and metabolite classes. Basic information about physicochemical methods applied to qualitative and quantitative analyses of biopolymers and natural products is also briefly presented.

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Enhanced Trehalose Production Improves Growth of Escherichia coli under Osmotic Stress

Cited by 171 publications

References 46 publications

Ethanol synthesis from glycerol by Escherichia coli redox mutants expressing adhE from Leuconostoc mesenteroides

Ethanol synthesis from glycerol by Escherichia coli redox mutants expressing adhE from Leuconostoc mesenteroides

Mesophilic Aeromonas UDP-glucose pyrophosphorylase (GalU) mutants show two types of lipopolysaccharide structures and reduced virulence

Influence of abiotic stresses on plant proteome and metabolome changes

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