2001
DOI: 10.1006/mthe.2001.0282
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Enhanced Transgene Expression in Cord Blood CD34+-Derived Hematopoietic Cells, Including Developing T Cells and NOD/SCID Mouse Repopulating Cells, Following Transduction with Modified TRIP Lentiviral Vectors

Abstract: The recent development of lentivirus-derived vectors is an important breakthrough in gene transfer technology because these vectors allow transduction of nondividing cells such as hematopoietic stem cells (HSC), due to an active nuclear import of reverse-transcribed vector DNA. We recently demonstrated that addition of the central DNA flap of HIV-1 to an HIV-derived lentiviral vector strikingly increases transduction of CD34(+) cells. We now describe improvements of the transduction protocol designed to preser… Show more

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Cited by 151 publications
(142 citation statements)
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“…27 The EF1a promoter was replaced by the tetracyclin-inducible pTRE promoter coming from the vector pTRE-tight (Clontech, Issy-Les-Moulineaux, France) and a multiple cloning site was inserted in place of the green fluorescent protein (GFP) gene (Supplementary Figure S4). The resulting lentivector plasmids were pTRIP-TRE-D133p53a, -D133P53b, and -D133P53g.…”
Section: Cells and Embryosmentioning
confidence: 99%
“…27 The EF1a promoter was replaced by the tetracyclin-inducible pTRE promoter coming from the vector pTRE-tight (Clontech, Issy-Les-Moulineaux, France) and a multiple cloning site was inserted in place of the green fluorescent protein (GFP) gene (Supplementary Figure S4). The resulting lentivector plasmids were pTRIP-TRE-D133p53a, -D133P53b, and -D133P53g.…”
Section: Cells and Embryosmentioning
confidence: 99%
“…In HIV-1, the central DNA flap is a 99-nucleotide overlap. This structure enhances the nuclear import of the PIC, increasing the transduction efficiency of lentiviral vectors (Sirven et al, 2000;Zennou et al, 2000). The increased efficiency of cPPT-containing vectors has been attributed at least in part to enhanced nuclear import (Van Maele et al, 2003;Zennou et al, 2000).…”
Section: Nuclear Importmentioning
confidence: 99%
“…These results are in accordance with two previously published papers, demonstrating an enhanced eGFP expression in human T cells transduced with EF1␣-containing lentivectors as compared with corresponding vectors containing an internal CMV-promoter. 18,21 Thus, LOX RANTES-SK was used for further analysis.…”
Section: Lentiviral Vector Construction and Intrakine Expression Drimentioning
confidence: 99%