Enhanced tolerance to salinity stress and ABA is regulated by Oryza sativa STRESS ASSOCIATED PROTEIN 8 (OsSAP8)

Roslan, Nur Farhana; Rashid, Nor Syafiqah Abd; Suka, Intan Elya; Taufik, Nadiatul Ain Nabila Ahmad; Abdullah, Nur Syahirah; Asruri, Mohd Bajuri; Toni, Bernadette; Sukiran, Noor Liyana; Zainal, Zamri; Isa, Nurulhikma Md

doi:10.21475/ajcs.17.11.07.pne505

Cited by 4 publications

(6 citation statements)

References 36 publications

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“…Moreover, expressing stress resistance genes under strong constitutive promoters may cost the fitness of transgenics due to the metabolic burden as demonstrated by rice lines overexpressing DREB1a , saltol zinc finger protein (STZ/ZAT10) and wax production 1 [ 8 ]. Rice transgenic lines overexpressing OsLEA3-1 [ 9 ] or OsSAP8 [ 10 ] display significantly higher yield or better physiological performance under stress than wild type (WT) but with compromised yield (up to ~50% yield reduction) under unstressed control conditions. To circumvent these problems, constitutive promoters of plant origin derived from highly active housekeeping genes ( ubiquitin, actin, tubulin and elf —eukaryotic initiation factor) have been used in transgenic plant development.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, Spartina alterniflora L.

Sengupta

Pehlivan

Mangu

et al. 2022

Biology

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Stress-inducible promoters are vital for the desirable expression of genes, especially transcription factors, which could otherwise compromise growth and development when constitutively overexpressed in plants. Here, we report on the characterization of the promoter region of a stress-responsive gene SaAsr1 from monocot halophyte cordgrass (Spartina alterniflora). Several cis-acting elements, such as ABRE (ABA-responsive element), DRE-CRT (dehydration responsive-element/C-Repeat), LTRE (low temperature-responsive element), ERE (ethylene-responsive element), LRE (light-responsive element), etc. contributed at varying degrees to salt-, drought- and ABA-enhanced expression of gusA reporter gene in Arabidopsis thaliana under the full-length promoter, pAsr11875 and its deletion derivatives with an assortment of cis-regulatory motifs. The smallest promoter, pAsr1491, with three cis-acting elements (a CCAAT box-heat responsive, an LRE, and a copper responsive element) conferred drought-enhanced expression of gusA; pAsr1755 (with an ABRE and a DRE) presented the highest expression in ABA and drought; and pAsr1994 with seven ABREs and two DREs conferred optimal induction of gusA, especially under drought and ABA. Arabidopsis transgenics expressing a known abiotic stress-responsive gene, SaADF2 (actin depolymerization factor 2), under both pAsr11875 and p35S promoters outperformed the wild type (WT) with enhanced drought and salt tolerance contributed by higher relative water content and membrane stability with no significant difference between pAsr11875:SaADF2 or p35S:SaADF2 lines. However, pAsr11875:SaADF2 lines produced healthy plants with robust shoot systems under salt stress and control compared to slightly stunted growth of the p35S:SaADF2 plants. This reestablished the evidence that transgene expression under a stress-inducible promoter is a better strategy for the genetic manipulation of crops.

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Section: Introductionmentioning

confidence: 99%

Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, Spartina alterniflora L.

Sengupta

Pehlivan

Mangu

et al. 2022

Biology

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“…Drought and salt stress lead to hyperosmotic signals and the accumulation of ABA which then triggers various adaptive responses in plants. This can be seen in the Roslan et al (2017) study, where OsSAP8 has a higher tolerance towards a high concentration of ABA by showing an insensitive germination phenotype while atsap2 showed a sensitive phenotype when applied with an even lower concentration of ABA. OsSAP8 is also a cytoplasmic protein, located in the nucleus and cytoplasm; and consists of both A20 and AN1 finger domains, which are essential in plant stress responses.…”

Section: Discussionmentioning

confidence: 71%

“…Plants were grown in a growth chamber at 22°C with 65% relative humidity and 16 h light/8 h dark photo cycle (He et al, 2019;Yang et al, 2019). After vegetative stress arose, 3 weeks-old seedlings were treated to control treatment (maintained without adding NaCl and consecutively watering), salinity treatment (200 mL of 200 mM NaCl), and drought treatment (withhold watering) for 3 weeks (seven days treatment, 14 days treatment & 21 days treatment) (Ströher et al, 2009;Roslan et al, 2017;Nutan et al, 2019). Both stresses have six individual plants for biological replicates.…”

Section: Arabidopsis Growth Condition and Stress Treatmentsmentioning

confidence: 99%

“…In this study, we also reported the effect of gene mutation using the OsSAP8 orthologue in Arabidopsis which is the AtSAP2. The AtSAP2 has been previously analyzed and showed high similarity in protein sequence to OsSAP8 (Roslan et al, 2017). High similarity in protein sequence may indicate that these two genes have similar functions in both plant species respectively.…”

Section: Introductionmentioning

confidence: 99%

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Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis

Mohd Roszelin,

Mohd Hazbir,

Jumali

et al. 2023

MAB

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Environmental stress can hinder the growth and development of crops, thereby reducing productivity. Plants can adapt to changing environments through various morpho-physiological changes, transcriptome regulation, signaling, translational and post-translational modifications. Stress Associated Proteins (SAPs) have been shown to play a crucial role in plant adaptation to biotic and abiotic stressors. They are encoded by a family of genes that produce a zinc finger protein with A20 and/or AN1 domains at either their N or C-terminal ends. Therefore, this study focused on understanding the role of the Oryza sativa SAP gene family (OsSAPs) in response to drought and salinity stress. In-silico analysis revealed that most of the OsSAP family members were upregulated by stress; two highly inducible OsSAP genes were also upregulated in response to stress under a rice-specific background. To study gene function, an Arabidopsis transformation system was employed using three genotypes: Col-0 (wild type), overexpressed transgenic OsSAP8, and atsap2 T-DNA knockout mutant. Arabidopsis AtSAP2 gene, which is homologous to rice OsSAP8, was used as a comparison to the loss of function mutation in Arabidopsis. Morphophysiological analysis showed that the atsap2 mutant displayed a sensitive phenotype to drought and salinity stress through low relative chlorophyll content and delayed inflorescence development and flowering as compared to Col-0 and transgenic OsSAP8. This suggests that the abolished atsap2 gene may contribute to reduced stress tolerancein plants. In contrast, transgenic OsSAP8 overexpression demonstrated tolerance to drought and salinity stress by maintaining relative chlorophyll content under both stress conditions, indirectly reflecting sustained photosynthetic machinery and stable photosynthetic rate. Further investigation, such as measuring the photosynthesis rate, is required to establish the correlation between chlorophyll data and photosynthesis activity.

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“…Overexpression of OsSAP1 in tobacco confers increased tolerance to abiotic stresses [ 70 ]. Enhanced tolerance to ABA and salt stress is regulated by OsSAP8 in Oryza sativa [ 71 ]. Therefore, peu-miR530b might participate in the response of ABA, as well as the tolerence of abiotic stresses mediated by bHLH or SAP1/11 in P. euphratica .…”

Section: Discussionmentioning

confidence: 99%

Exploration of ABA Responsive miRNAs Reveals a New Hormone Signaling Crosstalk Pathway Regulating Root Growth of Populus euphratica

Lian

Yao

Duan

et al. 2018

IJMS

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Abscisic acid (ABA) plays an important role in the regulation of plant adaptation, seed germination, and root development in plants. However, the mechanism of ABA regulation of root development is still poorly understood, especially through the miRNA-mediated pathway. Here, small RNA (sRNA)-seq and degradome-seq were used to analyze the miRNAs’ responsive to ABA in the stems and roots of P. euphratica, a model tree species for abiotic stress-resistance research. In total, 255 unique mature sequences, containing 154 known miRNAs and 101 novel miRNAs were identified, among which 33 miRNAs and 54 miRNAs were responsive to ABA in the roots and stems, respectively. Furthermore, the analysis of these miRNAs and their targets revealed a new hormone signaling crosstalk model of ABA regulation of root growth through miRNA-mediated pathways, such as peu-miR-n68 mediation of the crosstalk between ABA and the brassinosteroid (BR) signaling pathway and peu-miR477b mediation of the crosstalk between ABA and Gibberellic acid (GA) signaling. Taken together, our genome-wide analysis of the miRNAs provides a new insight into the mechanism of ABA regulation of root growth in Populus.

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Enhanced tolerance to salinity stress and ABA is regulated by Oryza sativa STRESS ASSOCIATED PROTEIN 8 (OsSAP8)

Cited by 4 publications

References 36 publications

Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, Spartina alterniflora L.

Characterization of a Stress-Enhanced Promoter from the Grass Halophyte, Spartina alterniflora L.

Characterization And Functional Study Of Stress-Associated Protein In Rice And Arabidopsis

Exploration of ABA Responsive miRNAs Reveals a New Hormone Signaling Crosstalk Pathway Regulating Root Growth of Populus euphratica

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