Enhanced Tolerance to Naphthalene and Enhanced Rhizoremediation Performance for Pseudomonas putida KT2440 via the NAH7 Catabolic Plasmid

Fernández, Matilde; Niqui-Arroyo, José Luis; Conde, Susana; Ramos, Juan L.; Duque, Estrella

doi:10.1128/aem.00619-12

Cited by 61 publications

(38 citation statements)

References 57 publications

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“…Some of the changes that can occur in the membrane, when bacteria are exposed to hydrophobic solvents, include vesicle formation, alteration of phospholipid composition, and reduced permeability of the cell membrane (Heipieper & De Bont, 1994; Nicolaou, Gaida, & Papoutsakis, 2010; Ramos et al, 2002). The mechanisms behind tolerance in the model strain P. putida KT2440 has also been studied for different compounds (Benndorf, Thiersch, Loffhagen, Kunath, & Harms, 2006; Domínguez‐Cuevas, González‐Pastor, Marqués, Ramos, & de Lorenzo, 2006; Fernandez, Conde et al, 2012; Fernandez, Niqui‐Arroyo, Conde, Ramos, & Duque, 2012; Roca, Rodríguez‐Herva, Duque, & Ramos, 2008; Santos, Benndorf, & Sá‐Correia, 2004). …”

Section: Introductionmentioning

confidence: 99%

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Calero

Jensen

Bojanovič

et al. 2017

Biotech & Bioengineering

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The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here, the tolerance of P. putida KT2440 toward eleven toxic chemical compounds was investigated. P. putida was found to be significantly more tolerant toward three of the eleven compounds when compared to Escherichia coli. Increased tolerance was for example found toward p‐coumaric acid, an interesting precursor for polymerization with a significant industrial relevance. The tolerance mechanism was therefore investigated using the genome‐wide approach, Tn‐seq. Libraries containing a large number of miniTn5‐Km transposon insertion mutants were grown in the presence and absence of p‐coumaric acid, and the enrichment or depletion of mutants was quantified by high‐throughput sequencing. Several genes, including the ABC transporter Ttg2ABC and the cytochrome c maturation system (ccm), were identified to play an important role in the tolerance toward p‐coumaric acid of this bacterium. Most of the identified genes were involved in membrane stability, suggesting that tolerance toward p‐coumaric acid is related to transport and membrane integrity.

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Section: Introductionmentioning

confidence: 99%

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Calero

Jensen

Bojanovič

et al. 2017

Biotech & Bioengineering

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“…The Gram-negative bacterium Pseudomonas putida KT2440 is an efficient colonizer of the root system of different plants that activates induced systemic resistance in the plant and can be used in rhizoremediation (18,19,20). P. putida KT2440 is also able to develop biofilms on biotic and abiotic surfaces, and molecular determinants participating in these processes have been described.…”

mentioning

confidence: 99%

Roles of Cyclic Di-GMP and the Gac System in Transcriptional Control of the Genes Coding for the Pseudomonas putida Adhesins LapA and LapF

2014

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LapA and LapF are large extracellular proteins that play a relevant role in biofilm formation by Pseudomonas putida. Current evidence favors a sequential model in which LapA is first required for the initial adhesion of individual bacteria to a surface, while LapF participates in later stages of biofilm development. In agreement with this model, lapF transcription was previously shown to take place at late times of growth and to respond to the stationary-phase sigma factor RpoS. We have now analyzed the transcription pattern of lapA and other regulatory elements that influence expression of both genes. The lapA promoter shows a transient peak of activation early during growth, with a second increase in stationary phase that is independent of RpoS. The same pattern is observed in biofilms although expression is not uniform in the population. Both lapA and lapF are under the control of the two-component regulatory system GacS/GacA, and their transcription also responds to the intracellular levels of the second messenger cyclic diguanylate (c-di-GMP), although in surprisingly reverse ways. Whereas expression from the lapA promoter increases with high levels of c-di-GMP, the opposite is true for lapF. The transcriptional regulator FleQ is required for the modulation of lapA expression by c-di-GMP but has a minor influence on lapF. This work represents a further step in our understanding of the regulatory interactions controlling biofilm formation in P. putida.

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“…For instance, P. aeruginosa can grow in the presence of a variety of aliphatic and aromatic compounds such as lactate (Gao et aromatic fl uoranthene, phenanthrene (Zhang et al 2011), hexadecane, benzene, toluene (S Mukherjee et al 2010), paracetamol (Hu et al 2013), and 4-chlorobenzoate (Hoskeri et al 2011). Similar wide range of assimilation of substrates has been reported with other species of the same genus, such as Pseudomanas putida with biodegradation of aromatic compounds (Diaz et al 2008, Ebrahimi and Plettner 2013, El-Naas et al 2009, Fernandez et al 2012, Hwang et al 2009, Li et al 2011, Q Lin and Jianlong 2010, Phale et al 2013, Takeo et al 2006, You et al 2013) and alkane derivatives (Dunn et al 2005, Johnson and Hyman 2006, Smith and Hyman 2004. In line with previous reports, Pseudomonas aeruginosa strain N7B1 had a wide range of substrate specifi city, thus is an important bacterium that could be used in bioremediation strategies.…”

Section: Substrate Utilizationmentioning

confidence: 60%

“…The second pathway has been studied the most, and genes encoding for these catabolic enzymes are generally found in bacterial extra-chromosome plasmids, and plasmids from several bacterial species have been A. Nzila, A. Thukair, S. Sankara, B. Chanbasha, M.M. Musa characterized, including the plasmid NAH7 of Pseudomonas putida strain G (Fernandez et al 2012), pAK5 of Pseudomonas putida strain AK5 (Izmalkova et al 2013), and plasmid of Gordonia sp. strain CC-NAPH129-6 (Izmalkova et al 2013).…”

Section: Detection Of Metabolites By Gc-msmentioning

confidence: 99%

Isolation and characterization of naphthalene biodegrading Methylobacterium radiotolerans bacterium from the eastern coastline of the Kingdom of Saudi Arabia

Nzila¹,

Thukair²,

Sankara³

et al. 2016

Archives of Environmental Protection

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Bioremediation is based on microorganisms able to use pollutants either as a source of carbon or in co-metabolism, and is a promising strategy in cleaning the environment. Using soil contaminated with petroleum products from an industrial area in Saudi Arabia (Jubail), and after enrichment with the polycyclic aromatic hydrocarbon (PAH) naphthalene, a Methylobacterium radiotolerans strain (N7A0) was isolated that can grow in the presence of naphthalene as the sole source of carbon. M. radiotolerans is known to be resistant to gamma radiation, and this is the fi rst documented report of a strain of this bacterium using a PAH as the sole source of carbon. The commonly reported Pseudomonas aeruginosa (strain N7B1) that biodegrades naphthalene was also identifi ed, and gas chromatography analyses have shown that the biodegradation of naphthalene by M. radiotolerans and P. aeruginosa did follow both the salicylate and phthalate pathways.

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Enhanced Tolerance to Naphthalene and Enhanced Rhizoremediation Performance for Pseudomonas putida KT2440 via the NAH7 Catabolic Plasmid

Cited by 61 publications

References 57 publications

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Genome‐wide identification of tolerance mechanisms toward p‐coumaric acid in Pseudomonas putida

Roles of Cyclic Di-GMP and the Gac System in Transcriptional Control of the Genes Coding for the Pseudomonas putida Adhesins LapA and LapF

Isolation and characterization of naphthalene biodegrading Methylobacterium radiotolerans bacterium from the eastern coastline of the Kingdom of Saudi Arabia

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