Enhanced tolerance to drought in transgenic rice plants overexpressing C4 photosynthesis enzymes

Gu, Junfei; Qiu, Ming; Yang, Jianchang

doi:10.1016/j.cj.2013.10.002

Cited by 57 publications

(33 citation statements)

References 44 publications

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“…However, different results have been published for soybean, showing asparaginase to be induced by low temperature, ABA and NaCl but not heat shock or drought stress (Cho et al, 2007). Pyruvate orthophosphate dikinase has been shown to be induced in the roots of rice seedlings during gradual drying, cold, high salt, and water deficit response (Moons, Valcke, & Van Montagu, 1998), and its overexpression in maize has been shown to improve drought tolerance (Gu, Qiu, & Yang, 2013).…”

Section: Asparagine Metabolism In Wheat Roots Under Drought Stressmentioning

confidence: 99%

Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress

Curtis

Bò

Tucker

et al. 2018

Food and Energy Security

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A detailed network describing asparagine metabolism in plants was constructed using published data from Arabidopsis (Arabidopsis thaliana) maize (Zea mays), wheat (Triticum aestivum), pea (Pisum sativum), soybean (Glycine max), lupin (Lupus albus), and other species, including animals. Asparagine synthesis and degradation is a major part of amino acid and nitrogen metabolism in plants. The complexity of its metabolism, including limiting and regulatory factors, was represented in a logical sequence in a pathway diagram built using yED graph editor software. The network was used with a Unique Network Identification Pipeline in the analysis of data from 18 publicly available transcriptomic data studies. This identified links between genes involved in asparagine metabolism in wheat roots under drought stress, wheat leaves under drought stress, and wheat leaves under conditions of sulfur and nitrogen deficiency. The network represents a powerful aid for interpreting the interactions not only between the genes in the pathway but also among enzymes, metabolites and smaller molecules. It provides a concise, clear understanding of the complexity of asparagine metabolism that could aid the interpretation of data relating to wider amino acid metabolism and other metabolic processes.

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Section: Asparagine Metabolism In Wheat Roots Under Drought Stressmentioning

confidence: 99%

Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress

Curtis

Bò

Tucker

et al. 2018

Food and Energy Security

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“…Consequently, the integration of the C 4 PPDK gene into C 3 plants could be a promising approach to relegate photorespiration, resulting in the improvement of photosynthetic efficiency of C 3 plants under climatic stress (Yadav and Mishra, 2020). In this current study, a gene that encoded PPDK was successfully introduced and expressed in a C 3 plant (Ku et al, 2000;Fukayama et al, 2001;Gu et al, 2013).…”

Section: Introductionmentioning

confidence: 94%

“…In addition, C 4 photosynthesis has an advantage over that of C 3 plants, exhibiting a higher assimilation efficiency and yield of biomass under different abiotic stress conditions, including drought, high temperature, and high light intensity, by improving water and nitrogen use efficiency (Hatch, 1987;Sage and Pearcy, 2000). Enzymes involved in the C 4 assimilation pathway could also play a significant role in tolerance to abiotic and biotic stress (Ku et al, 2000;Gu et al, 2013). Enhancing photosynthesis efficiency in C 3 plants using an enzyme from the C 4 pathway could provide an alternative approach for improving productivity, biomass, and yield (Yadav and Mishra, 2019).…”

Section: Introductionmentioning

confidence: 99%

The Pyruvate-Phosphate Dikinase (C4-SmPPDK) Gene From Suaeda monoica Enhances Photosynthesis, Carbon Assimilation, and Abiotic Stress Tolerance in a C3 Plant Under Elevated CO2 Conditions

2020

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A pyruvate-phosphate dikinase (C 4 -PPDK) gene was cloned from Suaeda monoica, which had a single-cell C 4 photosynthesis pathway without Kranz anatomy and was functionally validated in a C 3 model plant under different abiotic stress conditions in an ambient and elevated CO 2 environment. Overexpression of SmPPDK promoted growth of C 3 transgenic plants, enhancing their photosynthesis (CO 2 assimilation) by lowering photorespiration under stress conditions. Transgenic plants also showed an improved physiological status, with higher relative water content (RWC), membrane integrity, concentration of glycine betaine, total soluble sugars, free amino acids, polyphenols and antioxidant activity, and lower electrolyte leakage, lipid peroxidation, free radical accumulation, and generation of reactive oxygen species (ROS), compared to control plants. Moreover, SmPPDK transgenic plants exhibited earlier flowering and higher dry biomass compared to controls. These results suggested that the C 4 -PPDK gene was appropriate for improvement of carbon assimilation, and it also played an important role in adaption to salinity and severe drought-induced stress. More intriguingly, an elevated CO 2 environment alleviated the adverse effects of abiotic stress, particularly caused by drought through coordination of osmoprotectants and antioxidant defense systems. The molecular, physiological, metabolic, and biochemical indicators ameliorated the overall performance of model C 3 plants overexpressing the C 4 -PPDK gene in an elevated CO 2 environment, by lowering photorespiration metabolic processes, however, further studies are needed to confirm its precise role in C 3 plants as protection against future climate change.

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“…Although different from lower plants (algage), the CA of higher plants was also found have the similar function. According to Gu et al [17], the activities of phosphoenolpyruvate carboxylase and CA in two transgenic rice genotypes increased three-to five-fold compared to those in an untransformed wild type cultivar, with the same level of drought stress. Notably, the biomass production of the transgenic plants was 7%-15% higher than that of the wild type cultivar.…”

Section: Introductionmentioning

confidence: 99%

“…Using a method based on stable carbon isotopes, we quantified the proportion of bicarbonate use by plants [11]. Although the conversion to biomass from bicarbonate by the activity of CA has been proposed in studies of plant biomass production for energy [17,18,20], the mechanism and the capacity for bicarbonate use in plants in karst habitats are not fully understood. In this study, we hypothesized that CA activity of plants increased under drought stress, which produced H 2 O and CO 2 from the bicarbonate (HCO 3 − ) in limestone dissolution of karst soils for use in photosynthesis and biomass production.…”

Section: Introductionmentioning

confidence: 99%

Biomass Production of Three Biofuel Energy Plants’ Use of a New Carbon Resource by Carbonic Anhydrase in Simulated Karst Soils: Mechanism and Capacity

Wang

Xing

et al. 2017

Energies

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Abstract:To determine whether the bicarbonate in karst limestone soil could be used as a new carbon resource for biomass production by the catalysis of carbonic anhydrase (CA), a simulative karst drought stress experiment was designed and performed. Three plants used for biofuel energy,, and Euphorbia lathyris L. (El), were grown under simulated karst drought stress. In response to drought stress, the photosynthesis of the three energy plants was inhibited, but their CA activity increased. The hypothesis was confirmed by plant physiological and stable isotope techniques. The obtained results showed that plant biomass was produced with atmospheric CO 2 as well as bicarbonate under drought stress. Bicarbonate use was proportional to the CA activity of the plants. With high CA activity over a long period, El had the highest proportional bicarbonate use compared to Ov and Bj, reaching 26.95%. Additionally, a new method is proposed for the screening of plants grown for energy in karst habitats.

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Enhanced tolerance to drought in transgenic rice plants overexpressing C4 photosynthesis enzymes

Cited by 57 publications

References 44 publications

Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress

Construction of a network describing asparagine metabolism in plants and its application to the identification of genes affecting asparagine metabolism in wheat under drought and nutritional stress

The Pyruvate-Phosphate Dikinase (C4-SmPPDK) Gene From Suaeda monoica Enhances Photosynthesis, Carbon Assimilation, and Abiotic Stress Tolerance in a C3 Plant Under Elevated CO2 Conditions

Biomass Production of Three Biofuel Energy Plants’ Use of a New Carbon Resource by Carbonic Anhydrase in Simulated Karst Soils: Mechanism and Capacity

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