Enhanced SV40-virus replication in Chinese hamster kidney cells pretreated with 5-iodo-2?-deoxyuridine

Suárez, H. G.; Morris, A. G.; Lavialle, Christian; Cassingéna, R.

doi:10.1007/bf01320580

Cited by 6 publications

(4 citation statements)

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“…A typical example of non-permissive cells are rodent cells, that carry SV40 DNA integrated in their genome, while cells are transformed (62). Semi-permissive cells allow SV40 multiplication, but they produce a limited viral progeny (63). The majority of cells lyse and die upon infection, but a fraction of cells, which resist the SV40 infection, are transformed and immortalized, while producing a viral progeny at low titer.…”

Section: Sv40-mediated Cell Immortalization and Transformationmentioning

confidence: 99%

Association Between Simian Virus 40 and Human Tumors

et al. 2019

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Simian virus 40 (SV40) is a small DNA tumor virus of monkey origin. This polyomavirus was administered to human populations mainly through contaminated polio vaccines, which were produced in naturally infected SV40 monkey cells. Previous molecular biology and recent immunological assays have indicated that SV40 is spreading in human populations, independently from earlier SV40-contaminated vaccines. SV40 DNA sequences have been detected at a higher prevalence in specific human cancer specimens, such as the brain and bone tumors, malignant pleural mesotheliomas, and lymphoproliferative disorders, compared to the corresponding normal tissues/specimens. However, other investigations, which reported negative data, did not confirm an association between SV40 and human tumors. To circumvent the controversies, which have arisen because of these molecular biology studies, immunological researches with newly developed indirect ELISA tests were carried out in serum samples from patients affected by the same kind of tumors as mentioned above. These innovative indirect ELISAs employ synthetic peptides as mimotopes/specific SV40 antigens. SV40 mimotopes do not cross-react with the homologous human polyomaviruses, BKPyV, and JCPyV. Immunological data obtained from indirect ELISAs, using SV40 mimotopes, employed to analyze serum samples from oncological patients, have indicated that these sera had a higher prevalence of antibodies against SV40 compared to healthy subjects. The main data on (i) the biology and genetics of SV40; (ii) the epidemiology of SV40 in the general population, (iii) the mechanisms of SV40 transformation; (iv) the putative role of SV40 in the onset/progression of specific human tumors, and (v) its association with other human diseases are reported in this review.

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Section: Sv40-mediated Cell Immortalization and Transformationmentioning

confidence: 99%

Association Between Simian Virus 40 and Human Tumors

et al. 2019

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“…on September 25, 2020 by guest http://jvi.asm.org/ Downloaded from synthesizing T-antigen was not significantly changed by MC. This observation should be compared with the results obtained with IUdR, which enhances the growth of adenovirus 7 and SV40 upon treatment of cells semipermissive or permissive for these viruses (3,(10)(11)(12). In these two cases, it has been shown that IUdR pretreatment stimulates the percentage of T-antigensynthesizing cells and acts at a step preceding the expression of early viral functions.…”

Section: Notes Hours Ater Infectionmentioning

confidence: 96%

“…IUdR acts at an early step of the virus cycle, before T-antigen synthesis. Experiments performed to determine whether IUdR affects adsorption, penetration, or decapsidation of SV40 indicated that the analog acts between the arrival of virus DNA in the nucleus and the antigen production (11,12). MC enhancement of cell permissiveness may occur at a later step, intermediate between T-antigen production and SV40 DNA replication (4).…”

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confidence: 99%

“…Having observed the high efficiency of IUdR and MC in enhancing the permissiveness to SV40 in different cellular systems (4,11,12), we examined their combined effect on virus replication on previously characterized semipermissive Chinese hamster kidney cells. Upon infection with SV40 or virus DNA, Chinese hamster kidney cells supported virus DNA and virus synthesis at a low level; this cell system was defined as semipermissive (4).…”

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confidence: 99%

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