Enhanced Stress Tolerance in <i>Escherichia coli</i> and <i>Nicotiana tabacum</i> Expressing a Betaine Aldehyde Dehydrogenase/Choline Dehydrogenase Fusion Protein

Yilmaz, Jenny Lindberg; Bülow, Leif

doi:10.1021/bp020057k

Cited by 21 publications

(4 citation statements)

References 29 publications

(36 reference statements)

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“…Thus, under the burden of severe environment, it may involve in the protective response to external stress to maintain the cell shape. Similarly, glycine betaine is necessary for the survival of bacteria in diverse environment (Yilmaz and Bülow, 2002). Choline dehydrogenase oxidizes choline to betaine aldehyde and then further on to glycine betaine.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity and Mechanism of Action of Dracocephalum moldavica L. Extracts Against Clinical Isolates of Staphylococcus aureus

Yu¹,

Liu²,

Liu³

et al. 2019

Front. Microbiol.

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Background: Dracocephalum moldavica L. is a popular traditional medicine used by many countries, which has a wide range of pharmacological effects. The aim of this work was to investigate the antimicrobial effects of D. moldavica L. extracts against clinical isolates of Staphylococcus aureus . Our results demonstrated that the minimal inhibitory concentration (MIC) for 50 and 90% of S. aureus isolates (MIC 50 and MIC 90 ) of the ethyl acetate (EtOAc) fraction from D. moldavica L . ethanol extract were 780 and 1,065 μg/ml, respectively. We further observed that the EtOAc fraction disrupted 24-h biofilm caused cell membrane damage and irregular cell shape. Additionally, the EtOAc fraction showed slight to moderate toxic effects on human epidermal keratinocyte (HaCaT) cells. Moreover, the results of the differential proteome revealed that 231 proteins were upregulated, while 61 proteins were downregulated in S. aureus after treatment with the EtOAc fraction. The differentially expressed proteins were functionally categorized by the Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. These proteins contribute to membrane damage, inhibition of biofilm formation, and changes in energy metabolism. Thus, the EtOAc fraction of D. moldavica L. ethanol extract, as a natural product, has the potential to be used as an antimicrobial agent to control the clinical isolates of S. aureus .

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Activity and Mechanism of Action of Dracocephalum moldavica L. Extracts Against Clinical Isolates of Staphylococcus aureus

Yu¹,

Liu²,

Liu³

et al. 2019

Front. Microbiol.

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“…In the leaf beetle subtribe Chrysomelina sensu stricto, GMC oxidoreductases enabled chemical defenses and were important for adaptive processes in plant-insect interactions (Rahfeld et al, 2014). In E. coli, choline dehydrogenase catalyzes the flavin-dependent, two-step oxidation of choline to glycine betaine, which acts as an osmoprotectant compatible solute that accumulates when the cells are exposed to drastic environmental changes in osmolarity (Yilmaz and Bülow, 2010). However, little is known about the functional diversity of the rhizobium GMC family.…”

Section: Introductionmentioning

confidence: 99%

A GMC Oxidoreductase GmcA Is Required for Symbiotic Nitrogen Fixation in Rhizobium leguminosarum bv. viciae

Zou

Luo

et al. 2020

Front. Microbiol.

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GmcA is a FAD-containing enzyme belonging to the GMC (glucose-methanol-choline oxidase) family of oxidoreductases. A mutation in the Rhizobium leguminosarum gmcA gene was generated by homologous recombination. The mutation in gmcA did not affect the growth of R. leguminosarum, but it displayed decreased antioxidative capacity at H 2 O 2 conditions higher than 5 mM. The gmcA mutant strain displayed no difference of glutathione reductase activity, but significantly lower level of the glutathione peroxidase activity than the wild type. Although the gmcA mutant was able to induce the formation of nodules, the symbiotic ability was severely impaired, which led to an abnormal nodulation phenotype coupled to a 30% reduction in the nitrogen fixation capacity. The observation on ultrastructure of 4-week pea nodules showed that the mutant bacteroids tended to start senescence earlier and accumulate poly-β-hydroxybutyrate (PHB) granules. In addition, the gmcA mutant was severely impaired in rhizosphere colonization. Real-time quantitative PCR showed that the gmcA gene expression was significantly up-regulated in all the detected stages of nodule development, and statistically significant decreases in the expression of the redoxin genes katG, katE, and ohrB were found in gmcA mutant bacteroids. LC-MS/MS analysis quantitative proteomics techniques were employed to compare differential gmcA mutant root bacteroids in response to the wild type infection. Sixty differentially expressed proteins were identified including 33 up-regulated and 27 down-regulated proteins. By sorting the identified proteins according to metabolic function, 15 proteins were transporter protein, 12 proteins were related to stress response and virulence, and 9 proteins were related to transcription factor activity. Moreover, nine proteins related to amino acid metabolism were over-expressed.

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“…GPCho is a product of the breakdown of phosphatidylcholine and is converted to choline by GPCho phosphodiesterase. Choline dehydrogenase produces betaine aldehyde from choline [39], and glycine betaine is converted from betaine aldehyde by aldehyde dehydrogenase [40]. The mechanism underlying the downregulation of choline metabolism in the liver by PIC supplementation remains .…”

Section: Discussionmentioning

confidence: 99%

Metabolomic analyses of plasma and liver of mice fed with immature Citrus tumida peel

Toyoda

Satô

Muto

et al. 2020

Bioscience, Biotechnology, and Biochemistry

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In this study, we investigated the effects of dietary supplementation of Citrus tumida hort. ex Tanaka on food intake, body and fat tissue weights, and metabolic profiles of plasma and liver in mice. Supplementation with 5% (w/w) of peels of immature C. tumida (PIC) for 4 weeks significantly suppressed body weight gain and decreased adipose tissue weight in epididymal, perirenal, and subcutaneous fats. Metabolome analyses showed that 2-hydroxyvaleric acid levels were reduced in the blood plasma of mice fed with PIC. PIC supplementation significantly elevated dipeptide (Thr-Asp, Ser-Glu, and Ala-Ala), glucuronic acid, and S-methylglutathione levels, and significantly reduced betaine aldehyde levels in the liver. In conclusion, PIC supplementation affects the metabolism of fatty acids, pectin, glutathione, and choline, showing potential beneficial effects for metabolic syndrome and obesity. PIC may be developed as a functional food and used in the treatment of these diseases.

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Enhanced Stress Tolerance in Escherichia coli and Nicotiana tabacum Expressing a Betaine Aldehyde Dehydrogenase/Choline Dehydrogenase Fusion Protein

Cited by 21 publications

References 29 publications

Antimicrobial Activity and Mechanism of Action of Dracocephalum moldavica L. Extracts Against Clinical Isolates of Staphylococcus aureus

Antimicrobial Activity and Mechanism of Action of Dracocephalum moldavica L. Extracts Against Clinical Isolates of Staphylococcus aureus

A GMC Oxidoreductase GmcA Is Required for Symbiotic Nitrogen Fixation in Rhizobium leguminosarum bv. viciae

Metabolomic analyses of plasma and liver of mice fed with immature Citrus tumida peel

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