Enhanced starch hydrolysis using α-amylase immobilized on cellulose ultrafiltration affinity membrane

Konovalova, V. V.; Гузикевич, К.; Бурбан, Анатолій; Kujawski, Wojciech; Jarzynka, Karolina; Kujawa, Joanna

doi:10.1016/j.carbpol.2016.07.065

Cited by 40 publications

(23 citation statements)

References 52 publications

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“…The significant differences in the yields and chemical compositions of bamboo shoot powder (BSP), BSCDF and BSEDF were shown in Table 1. The yield of BSCDF (20.06%) was higher than BSEDF (16.11%), which might be due to the insufficient degradation of starch as Konovalova et al (2016) had reported that α-amylase could rapidly increase starch hydrolysis. This make chemical extraction more preferable for better yield.…”

Section: Component Analysismentioning

confidence: 92%

Physicochemical and Functional Properties of Dietary Fiber From Bamboo Shoots (Phyllostachys Praecox)

Wang

Zhu

et al. 2017

Emir J Food Agric

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Dietary fiber are non-digestible constituents of plant cell walls, and comprise a necessary component of diet and its positive connection with human health. Till now, there is no report about the extraction and physicochemical and functional properties of dietary fiber from bamboo shoots. To find the potential applications of dietary fiber from bamboo shoots in food and health products, the effects of chemical, enzymatic methods and particle size distribution on the chemical and structural composition, physicochemical, and functional properties of dietary fibers (DFs) from bamboo shoots were studied. The results showed that BSEDF and BSCDF had higher total DF and higher soluble DF, respectively. The crystalline regions calculated to be higher in latter and both had irregular surfaces and diverse monosaccharide composition. Both fibers showed good functional properties [water retention capacity (WRC) (11.24-15.13g/g), water swelling capacity (WSC) (18.84-28.75 mL/g), oil holding capacity (OAC) (6.71-10.15 g/g), glucose adsorption capacity (GAC) (0.08-6.89 mmol/g) and glucose retardation index (GRI) (3.57-40.92%)]. WRC of BSCDF and BSEDF decreased with the increase in the mesh size (40-200) while, WSC and OAC increased with mesh sizes (40 to 120), followed by decrease above mesh120. Both particle size and extraction methods significantly affected GRI. In conclusion, physico-chemical properties of fibers can be manipulated through treatments (chemical and enzymatic) to improve their overall functionality. Therefore, both BSCDF and BSEDF can find potential applications in food and health products as a functional ingredient in different aspects.

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Section: Component Analysismentioning

confidence: 92%

Physicochemical and Functional Properties of Dietary Fiber From Bamboo Shoots (Phyllostachys Praecox)

Wang

Zhu

et al. 2017

Emir J Food Agric

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“…This inhibition can be alleviated by adding β-glucosidase to cellulases, by converting the cellobiose to glucose. 13 Previous studies have examined the enzymatic hydrolysis of starch material using α-amylase and amyloglucosidase, [14][15][16][17][18] but the comparison between addition of these enzymes separately or in combination as hydrolyzing agents for algal starch has not been studied yet. The enzymatic hydrolysis of cellulosic material has been extensively investigated, [19][20][21][22][23] but there has been no attempt to describe the role of β-glucosidase in the hydrolysis of algal cellulose to glucose.…”

Section: Enzymatic Hydrolysis Of Algaementioning

confidence: 99%

Synergism of cellulases and amylolytic enzymes in the hydrolysis of microalgal carbohydrates

Shokrkar

Ebrahimi

2018

Biofuels Bioprod Bioref

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This study aimed to evaluate the synergism of cellulases and amylolytic enzymes in the hydrolysis of microalgal carbohydrates for bioethanol production. The effect of β‐glucosidase on the hydrolysis of algal cellulose, and the comparison between the effect of the addition of α‐amylase and amyloglucosidase separately or together on the hydrolysis of algal starch, have not been described previously. Results showed that β‐glucosidases promoted the enzymatic hydrolysis of algal cellulose as a result of the increase in the glucose production rate and the decrease in the inhibition by cellobiose. It was also beneficial to carry out the hydrolysis of algal starch using the mixture of α‐amylase and amyloglucosidase. The results obtained revealed that, in enzymatic hydrolysis experiments, simultaneous treatment with Celluclast 1.5 L, β‐glucosidase, α‐amylase, and amyloglucosidase was a promising strategy to reduce the hydrolysis time required to achieve a higher yield of glucose. Subsequently, fermentation of enzymatic hydrolysates of microalgae using Saccharomyces cerevisiae showed a yield of 0.126 g ethanol / g dried algae. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…PGA could be immobilized by adsorption method, cross‐linking method, and covalent bonding method . Among them, covalent binding method was favored by researchers due to strong binding force between PGA and target, abundant types of functional groups available for design, high utilization ratio, and so on …”

Section: Introductionmentioning

confidence: 99%

The engineering and immobilization of penicillin G acylase onto thermo‐sensitive tri‐block copolymer system

Liu

Zhang

et al. 2018

Polymers for Advanced Techs

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In this work, the relationships between catalytic performances of penicillin G acylase (PGA) and the molar ratio of carrier, thermo‐sensitive tri‐block polymer, poly (N,N‐diethylacrylamide‐b‐ β‐hydroxyethyl methacrylate‐b‐glycidyl methacrylate) (PDEA‐b‐PHEMA‐b‐PGMA) were studied firstly, and result documented the optimal molar ratio was nDEA:nHEMA:nGMA = 100:47:24, which presented a suitable lower critical solution temperature (LCST) of 35°C and the activity retention ratio of 80.62% (±0.50%). Based on the suitable carrier, immobilization conditions were investigated and optimized. When pH of solution, concentration of PGA, immobilized time, and immobilization temperature were 8.0, 1/10 (m/v), 16 hours, and 36°C, respectively, enzyme loading capacity (L), enzyme activity (Ea), and activity retention ratio (Ar) of PGA arrived at the highest value of 21 223 U, 16 199 U/g, and 93.50% (±0.50%), respectively. Besides, the response rate (Rr) of immobilized PGA was the same as free PGA, the reusable stability (Rs) was 77.00% (±1.00%) after using for 11 times, which indicated that the carrier has better compatibility with L, Ar, Rs, and Rr.

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Enhanced starch hydrolysis using α-amylase immobilized on cellulose ultrafiltration affinity membrane

Cited by 40 publications

References 52 publications

Physicochemical and Functional Properties of Dietary Fiber From Bamboo Shoots (Phyllostachys Praecox)

Physicochemical and Functional Properties of Dietary Fiber From Bamboo Shoots (Phyllostachys Praecox)

Synergism of cellulases and amylolytic enzymes in the hydrolysis of microalgal carbohydrates

The engineering and immobilization of penicillin G acylase onto thermo‐sensitive tri‐block copolymer system

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