Enhanced stability and antibacterial efficacy of a traditional Chinese medicine-mediated silver nanoparticle delivery system

Abstract

Silver nanoparticles (AgNPs) are widely used as antibacterial products in various fields. Recent studies have suggested that AgNPs need an appropriate stabilizer to improve their stability. Some antibacterial traditional Chinese medicines (TCMs) contain various reductive components, which can not only stabilize AgNPs but also enhance their antimicrobial activity. In this study, we developed a series of novel AgNPs using a TCM extract as a stabilizer, reducing agent, and antimicrobial agent (TCM-AgNPs). A storage stability investigation of the TCM-AgNPs suggested a significant improvement when compared with bare AgNPs. Further, conjugation of TCMs onto the AgNP surface resulted in stronger antimicrobial potency on antibacterial evaluation using Pseudomonas aeruginosa , Staphylococcus epidermidis , and Staphylococcus aureus with minimum inhibitory concentration 50% (MIC 50 ) ratios (and minimum bactericidal concentration 90% [MBC 90 ] ratios) of AgNPs to respective TCM-AgNPs as assessment indices. Among these, P. cuspidatum Sieb. et-conjugated AgNPs ( P.C. -AgNPs) had the advantage of a combination of TCMs and AgNPs and was studied in detail with regard to its synthesis and characterization. The extraction time, reaction temperature, and concentrations of AgNO 3 and Polygonum cuspidatum Sieb. et extract were critical factors in the preparation of P.C. -AgNPs. Further, the results of X-ray diffraction and Fourier transform infrared spectroscopy indicated successful preparation of P.C. -AgNPs. In representative studies, P.C. -AgNPs showed a well-defined spherical shape, a homogeneous small particle size (36.78 nm), a narrow polydispersity index (0.105), and a highly negative zeta potential (−23.6 mV) on transmission electron microscopy and dynamic light scattering. These results indicate that TCM-AgNPs have a potential role as antibacterial agents in the clinic setting.

Phytosynthesis of Silver Nanoparticles Using Perilla frutescens Leaf Extract: Characterization and Evaluation of Antibacterial, Antioxidant, and Anticancer Activities

“…41 The x-ray diffraction (XRD) pattern of PF@AgNPs was recorded in the 2θ range of 10° to 90° using Bruker D8 Advance powder XRD (Bruker AXS GmbH, Karlsruhe, Germany). 42 Thermogravimetric analysis (TGA) and derivative thermogravimetric analysis (DTA) were performed using a TGA-1 analyzer (Mettler Toledo, Switzerland). 43 The shapes and sizes of PF@AgNPs and the selected area electron diffraction (SAED) pattern of PF@AgNPs were determined using transmission electron microscopy (TEM).…”

Preparation of berberine hydrochloride-Ag nanoparticle composite antibacterial dressing based on 3D printing technology

“…Sun et al wrapped agrimony, rheum palmatum, scleromitrion diffusum, polygonum cuspidatum and other traditional Chinese medicines on the surface of Ag NP respectively to synthesize nano-inclusion compound of corresponding drugs. 5 Among them, the antibacterial activity of polygonum cuspidatum-Ag nano-inclusion compound against Pseudomonas aeruginosa was 3000 times stronger than that of polygonum cuspidatum extract, and the antibacterial activity of geranium-Ag nano-inclusion compound was also significantly improved. Yang et al combined Ag NP with extracts of the medicinal plant Chinese honeysuckle, Lonicera japonica Thunb.…”

Berberine/Ag nanoparticle embedded biomimetic calcium phosphate scaffolds for enhancing antibacterial function

“…Recent research studies indicate that certain traditional Chinese medicine (TCM) have an inhibitory activity on bacteria and some might even reverse bacterial resistance [7,8]. Coptidis rhizoma is one of the most frequently used herbs prescribed for treating bacterial gastroenteritis.…”