Enhanced squalene production by modulation of pathways consuming squalene and its precursor

Tominaga, Masahiro; Miyazaki, Kôji; Hataya, Shoko; Mitsui, Yuichi; Kuroda, S.; Kondo, Akihiko; Ishii, Jun

doi:10.1016/j.jbiosc.2022.04.004

Cited by 7 publications

(1 citation statement)

References 29 publications

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“…[20]. Squalene was detected at 210 nm using mobile phase methanol at 40 • C [21]. An ultraviolet/visible spectrophotometer was used to determine the absorbance value (OD 600 ) of the fermentation broth and detect the growth of S. cerevisiae.…”

Section: Extraction and Detection Methodsmentioning

confidence: 99%

Metabolic Engineering of Saccharomyces cerevisiae for Efficient Retinol Synthesis

Wang

Liu

et al. 2023

JoF

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Retinol, the main active form of vitamin A, plays a role in maintaining vision, immune function, growth, and development. It also inhibits tumor growth and alleviates anemia. Here, we developed a Saccharomyces cerevisiae strain capable of high retinol production. Firstly, the de novo synthesis pathway of retinol was constructed in S. cerevisiae to realize the production of retinol. Second, through modular optimization of the metabolic network of retinol, the retinol titer was increased from 3.6 to 153.6 mg/L. Then, we used transporter engineering to regulate and promote the accumulation of the intracellular precursor retinal to improve retinol production. Subsequently, we screened and semi-rationally designed the key enzyme retinol dehydrogenase to further increase the retinol titer to 387.4 mg/L. Lastly, we performed two-phase extraction fermentation using olive oil to obtain a final shaking flask retinol titer of 1.2 g/L, the highest titer reported at the shake flask level. This study laid the foundation for the industrial production of retinol.

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Section: Extraction and Detection Methodsmentioning

confidence: 99%