Enhanced specific antibody productivity of calcium alginate-entrapped hybridoma is cell line-specific

Lee, Gyun Min; Kim, Sang Jick; Palsson, Bernhard Ø.

doi:10.1007/bf00761774

Cited by 11 publications

(12 citation statements)

References 23 publications

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“…Several methods of enhanced productivity of monoclonal antibody, which was achieved by increasing the cell concentration and/or the specific productivity of monoclonal antibody, were investigated by sev-eral researchers (Lee et al, 1994;Yamaguchi et al, 1997;Takahashi et al, 1994;Murakami et al, 1997). Productivity often depends on the components of culture media containing autocrine factors (Toyoda et al, 1990;Sugahara et al, 1991), extracts from plants and cells (Kim et al, 1993), and other components (Mochizuki et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Untitled

et al. 1999

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Human colorectal adenocarcinoma tumor (CW2) cells were cultivated in RPMI 1640 media containing 0-7.5 mM aspirin and 10% fetal bovine serum for the production of carcinoembryonic antigen (CEA). By adding aspirin to the media, the production of CEA per cell increased by up to one hundred fold compared to cultivation in normal media containing no aspirin, even though the total cell concentration decreased with the increase in aspirin in the media. The production of CEA was also investigated for CW2 cells cultured on silk fibroin, poly(gamma-benzyl-L-glutamate) and poly(gamma-benzyl-L-glutamate)/poly(ethylene oxide) diblock copolymer films prepared by the Langmuir-Blodgett and casting methods. The highest production of CEA per cell was observed for the CW2 cells on poly(gamma-benzyl-L-glutamate) and its diblock copolymer films prepared by the Langmuir-Blodgett method in the medium containing 5 mM aspirin after 168 hr of inoculation. This originates from the fact that the cell density on the films in the medium containing 5 mM aspirin was the lowest under these conditions. It is suggested that CW2 cells produce CEA more effectively when the cell growth is suppressed by addition of toxic chemicals such as aspirin or by culture on unfavorable films for cell growth.

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Section: Introductionmentioning

confidence: 99%

Untitled

et al. 1999

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“…Cell microencapsulation is an alternative to lifelong immunosuppresion for prevention of graft failure and autoimmune destruction. Moreover, immobilization of hybridoma cells provides some intrinsic advantages over common free suspension culture, in particular cell protection against mechanical stress, easier antibody puri®cation (Scheirer et al, 1984), and higher monoclonal antibody production resulting from higher local and overall cell concentrations (Lee et al, 1993;Lee et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Microencapsulation of an anti‐VE–cadherin antibody secreting 1B5 hybridoma cells

Orive

Hernández

Rodríguez-Gascón

et al. 2001

Biotech & Bioengineering

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Accumulating experimental evidence demonstrates that tumor growth and lethality are dependent on angiogenesis. Based on this concept, there is growing interest in the use of antiangiogenesis agents to inhibit tumor expansion. Compelling data implicate vascular endothelium (VE)-cadherin (an endothelium specific protein) as a key factor in the last step of angiogenesis, where the endothelial cells join one to each other and form microtubules (future blood vessels). We propose a novel approach to the inhibition of angiogenesis by immobilizing VE-cadherin-secreting hybridoma cells in alginate-agarose microcapsules. Hybridoma cells can be protected with biocompatible and semipermeable membranes that permit exit of anti-VE-cadherin monoclonal antibodies but not entry of cellular immune mediators. Stability studies were performed to select the suitable microcapsule for cell immobilization. Alginate and agarose solid beads coated with poly-L-lysine and alginate were chosen according to their stability and diffusional properties. 1B5 hybridoma cells were grown within the microcapsules and secreted anti-VE-cadherin antibodies during the 9 days of culture, reaching a cumulative concentration of 1.7 microg/mL. This antibody concentration inhibited microtubule formation (87%) in the in vitro angiogenesis Matrigel assay. Moreover, the antiangiogenic effect observed was antibody concentration related. These findings open a new alternative for the inhibition or prevention of angiogenesis and demonstrates the feasibility of using microencapsulated cells as a control-drug delivery system.

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“…The batch bioreactor is considered here in the alternative with using immobilized biomass from several reasons: I) due to the reported biomass higher stability [26,32], and ii) to eventually consider its fed-batch operation {not approached in this paper [30]}. The adopted kinetic model for mAb production by using hybridoma cell culture is presented in Table 2 together with the associated rate constants.…”

Section: Bioprocess / Bioreactor Dynamic Model Bioprocess Kinetic Modelmentioning

confidence: 99%

In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture

Maria¹,

Gîjiu²,

Cebanu³

et al. 2019

Rev. Chim.

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Bioreactor optimization is a common engineering problem difficult to be solved due to the large number of influential variables of high variability. Production of monoclonal antibody is a well-known method to synthesize a large number of identical antibodies (that is of uniform characteristics, also called monoclonal antibodies, mAb). Due to such reasons intense efforts have been invested to maximize the production of mAb by using hybridoma cell culture. Based on an adequate kinetic model from literature (experimentally checked) this paper focus on pointing-out the major role of the net evolution of the viable biomass (growth, and decay) in the location of the optimal operating setpoint (SP) of a three-phase mechanically agitated batch bioreactor (TPMAB) with immobilized hybridoma culture. This in-silico analysis opens the possibility I) to optimize the bioreactor performances by placing the SP in the most favourable location, by adjusting the substrate and biomass initial load in the bioreactor according to the preliminary determined characteristics of a modified / improved biomass; ii) to optimize the batch-to-batch operation mode (not approached here) according to the time-varying characteristics of the biomass culture, or iii) to determine the optimal operation of the bioreactor in a fed-batch operating mode (not approached here).

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Enhanced specific antibody productivity of calcium alginate-entrapped hybridoma is cell line-specific

Cited by 11 publications

References 23 publications

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Microencapsulation of an anti‐VE–cadherin antibody secreting 1B5 hybridoma cells

In-silico Optimization of a Batch Bioreactor for mAbs Production in Relationship to the Net Evolution of the Hybridoma Cell Culture

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