Enhanced specialized metabolite, trichome density, and biosynthetic gene expression in <i>Stevia rebaudiana</i> (Bertoni) Bertoni plants inoculated with endophytic bacteria <i>Enterobacter hormaechei</i>

Oviedo-Pereira, Dumas G.; López-Meyer, Melina; Evangelista‐Lozano, Silvia; Sarmiento-López, Luis G.; Sepúlveda-Jiménez, Gabriela; Rodrı́guez-Monroy, Mario

doi:10.7717/peerj.13675

Cited by 15 publications

(10 citation statements)

References 69 publications

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“…Recent studies reported the relationship between trichome development in context to the accumulation of SGs, flavonoids, and polyphenols in plants of S. rebaudiana under biotic interactions. , Phytoconstituents secreted by GTs have industrial use; therefore, several strategies have been used to enhance their production.…”

Section: Introductionmentioning

confidence: 99%

Influence of Plant Growth Regulators on Glandular Trichome Density and Steviol Glycosides Accumulation in Stevia rebaudiana

et al. 2022

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With the advent of modern lifestyles, diabetes-related comorbidities attributed the importance of low-caloric natural sweetener plants such as Stevia rebaudiana . This plant is the viable source of steviol glycosides (SGs) and other economically important secondary metabolites. Glandular trichomes (GTs) play the role as a reservoir for all secondary products present in the plant species. Therefore, the present study was carried out to evaluate the influence of different plant growth regulators (PGRs) on GT density and its impact on the SG content. The direct shoot regeneration system was developed on Murashige and Skoog (MS) + benzyl aminopurine (BAP) (1.0 mg/L) + naphthaleneacetic acid (NAA) (0.5 mg/L), and MS + BAP (1.5 mg/L) + NAA (0.5 mg/L) from nodal and leaf explants, respectively. Among the combination of PGRs used, MS medium fortified with BAP (1.0 mg/L) and 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5 mg/L) played a significant role in increasing the GT density on leaf and stem tissues of S. rebaudiana . Furthermore, high-performance thin-layer chromatography and gas chromatography–mass spectrophotometry data confirmed a notable rise in SGs and other valuable secondary metabolites. Thus, the protocol developed can be used for the propagation of stevia with an improved metabolic profile at a large scale.

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Section: Introductionmentioning

confidence: 99%

Influence of Plant Growth Regulators on Glandular Trichome Density and Steviol Glycosides Accumulation in Stevia rebaudiana

et al. 2022

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“…These roles are mainly performed by secreting growth regulators and consequently helping to improve nutrition. Some previous papers have described the isolation of endophytic bacteria from different organs of stevia plants [34][35][36]; however, until now there has been no information about the endophytic bacteria inhabiting the stevia seeds. Here, for the first time, we report on bacteria isolated from Stevia rebaudiana seeds.…”

Section: Discussionmentioning

confidence: 99%

“…These genes encode UDP-glycosyltransferases, which are engaged in the last stages of the SGs biosynthesis pathway. Additionally, in the research of Oviedo-Pereira et al [35], reinoculation with the endophytic bacteria Enterobacter hormaechei increased SG synthesis, as well as flavonoid accumulation in the trichomes of S. rebaudiana plants. However, there is no information about the endophytic bacteria that occupy stevia seeds.…”

Section: Introductionmentioning

confidence: 94%

“…Endophytic bacteria have been isolated from the leaves, stems, roots, flowers, fruits, and seeds of various plant species [30][31][32][33]. In light of this information, it is not surprising that endophytic bacteria have also been identified in stevia plants [34,35]. As reported by Yu et al [36], in the seedling stage, the dominant genera of the endophytic bacteria of stevia are Enterobacterium and Erwinia, while Methylobacterium and Sphingomonas were found to be the principal endophytes in mature leaves.…”

Section: Introductionmentioning

confidence: 99%

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Seeds of Stevia rebaudiana Bertoni as a Source of Plant Growth-Promoting Endophytic Bacteria with the Potential to Synthesize Rebaudioside A

Simlat

Ptak

Jaglarz

et al. 2023

IJMS

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In this study, a new strain of Pantoea vagans, SRS89, was isolated from surface-sterilized stevia seeds. The isolate was evaluated using morphological, molecular, and biochemical methods. The bacterium was 1.5 μm long, yellowish in color, and classified as Gram-negative. Whole genome sequencing of our strain revealed the presence of a 4,610,019 bp chromosome, and genome annotation resulted in the detection of 4283 genes encoding 4204 putative coding sequences. Phylogenic analysis classified the genome of our strain close to the MP7 and LMG 24199 strains of P. vagans. Functional analysis showed that the highest number of genes within the analyzed bacterium genome were involved in transcription, amino acid transport and metabolism, and carbohydrate transport and metabolism. We also identified genes for enzymes involved in the biosynthesis of carotenoids and terpenoids. Furthermore, we showed the presence of growth regulators, with the highest amount noted for gibberellic acid A3, indole-3-acetic acid, and benzoic acid. However, the most promising property of this strain is its ability to synthesize rebaudioside A; the estimated amount quantified using reversed-phase (RP)-HPLC was 4.39 mg/g of the dry weight of the bacteria culture. The isolated endophytic bacterium may be an interesting new approach to the production of this valuable metabolite.

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“…PGPRs have various plant growth enhancement abilities, such as IAA (Indole-3-acetic acid) production, nitrogen fixation, and siderophore production (Herrera-Quiterio et al, 2020;Andleeb et al, 2022). Certain genera of bacteria, such as Bacillus, Pseudomonas, and Streptomyces are known as PGPR functional bacteria with direct or indirect mechanisms to enhance plant growth (Bokhari et al, 2019;Fidan and Zhan, 2019;Oviedo-Pereira et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Endophytic Streptomyces population induced by L-glutamic acid enhances plant resilience to abiotic stresses in tomato

Kim

Kwak

2023

Front. Microbiol.

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Endophyte bacteria, which colonize plants including roots, stem, flower, and fruit, it can derive their nutrients from the host, are recognized for their mutualistic relationship with the host plant. They play a critical role in promoting host growth and modulating abiotic stress. Carbon and nitrogen have a significant impact on bacterial population and secondary metabolite production, which are highly specific in various categories such as bacterial growth regulation, anti-compounds production. Application of L-glutamic acid can significantly enhance Streptomyces globisporus population buildup in plants. However, the effectiveness of this population buildup against abiotic stresses such as salinity and drought has not been investigated. Therefore, in this study, we tested the bacteria and their prebiotic activity against salinity and drought stress in tomato plants. Three different amino acids were treated on the tomato plants, and it was observed that L-asparagine and L-proline had a negative effect on plant growth and phenotype, while L-glutamic acid promoted plant growth and increased bacteria population density. The bacteria were found to colonize the rhizosphere and root endosphere, with colonization being promoted by L-glutamic acid. Additionally, Streptomyces was found to have plant growth promotion effects and provided protection against abiotic stresses. Interestingly, L-glutamic acid reduced the damage caused by salinity stress, but not drought stress. These findings suggest that L-glutamic acid plays a role in providing tolerance to salinity stress with the core microbiota, thus the current study demonstrated their prebiotic activity in the agriculture system.

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Enhanced specialized metabolite, trichome density, and biosynthetic gene expression in Stevia rebaudiana (Bertoni) Bertoni plants inoculated with endophytic bacteria Enterobacter hormaechei

Cited by 15 publications

References 69 publications

Influence of Plant Growth Regulators on Glandular Trichome Density and Steviol Glycosides Accumulation in Stevia rebaudiana

Influence of Plant Growth Regulators on Glandular Trichome Density and Steviol Glycosides Accumulation in Stevia rebaudiana

Seeds of Stevia rebaudiana Bertoni as a Source of Plant Growth-Promoting Endophytic Bacteria with the Potential to Synthesize Rebaudioside A

Endophytic Streptomyces population induced by L-glutamic acid enhances plant resilience to abiotic stresses in tomato

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